Salient features of nonsaccharolytic and weakly saccharolytic nonfermentative rods

Abstract

One hundred and thirty-one strains of nonsaccharolytic and weakly saccharolytic Gram-negative rods recently isolated from clinical specimens were examined with a battery of 74 tests, mostly biochemical. All but five of these strains were thus assigned to established taxa. Alkalinization of amides and organic acids was a particularly useful feature for identifying these bacteria.

Taxonomy of Bordetella and Related Organisms of the Families Achromobacteraceae, Brucellaceae, and Neisseriaceae

“…This distinguishes them from the nonmotile, oxidase-negative strains which we believe are misidentified strains of A cinetobacter Iwoffii. (58) found differences in the production of alkali from certain amides and organic salts, and these may prove useful after further evaluation. There is abundant evidence for retaining B. bronchiseptica in the same genus as B. pertussis and B. parapertussis on physiological, biochemical, serological, and genetic grounds (20,28,46,64,66).…”

Monoclonal Antibody for an Outer Membrane Lipoprotein of the Pseudomonas fluorescens Group of the Family Pseudomonadaceae

“…Currently, the identification of Pseudomonas species in clinical laboratories requires a large number of biochemical tests (12,13). These methods are time consuming, and some tests, especially those used to differentiate glucose-oxidizing Pseudomonas species, are not entirely satisfactory (6,12,13).…”

“…These methods are time consuming, and some tests, especially those used to differentiate glucose-oxidizing Pseudomonas species, are not entirely satisfactory (6,12,13). In recent years techniques that involve determinations (of deoxyribonucleic acid base composition (4), deoxyribonucleic acid-deoxyribonucleic acid hybridization (lo), ribonucleic acid homology (1, ll), or fatty acid composition (6, 7) have been developed.…”

The characterisation of Bordetella/Alcaligenes‐like organisms and their effects on Turkey Poults and chicks

“…This is not surprising, since there are differences in the basal media, the time taken to reach an end-point, and in the end-point itself. Pickett and Pederson (1970), Otto and Pickett (1976), Pickett (1980), Kiredjian et al (1981), Rimler and Simmons (1983), Berkoff and Riddle (1984), Kersters et al (1984) and Kreig and Holt (1984) all record the results of biochemical characterisation of Alcaligenes faecalis, Bordetella bronchiseptica, Bordetella avium and the alcaligenes-like rhinotracheitis bacterium described in the United States. It is apparent from their results that there is no consensus opinion of the properties of these bacteria, although the test conditions and the media seem to be comparable; OLP or OLP and the API system.…”

“…DISCUSSION Bacteria characterised broadly as Alcaligenes/Bordetella were termed 'genera of uncertain affiliation' by Buchanan and Gibbons (1974) and this phrase generally reflects the controversy about their taxonomic position and the efforts which have been made to define the limits of genera and species more precisely (Pickett and Pederson, 1970;Johnson andSneath, 1973, andKiredjian et al, 1981). In the current experiments, the conventional tests, including both the standard tests and further biochemical tests, using mainly methods described in Cowan and Steel, gave unfailingly reproducible results.…”