Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China

Abstract

A haloalkaliphilic strain XQ-INN 246T was isolated from the sediment of a salt pond in Inner Mongolia Autonomous Region, China. Cells of the strain were rods, motile and strictly aerobic. The strain was able to grow in the presence of 2.6–5.3 M NaCl (optimum concentration is 4.4 M) at 30–50 °C (optimum temperature is 42 °C) and pH 7.0–10.0 (optimum pH is 8.0–8.5). The whole genome sequencing of strain XQ-INN 246T revealed a genome size of 4.52 Mbp and a DNA G+C content of 62.06 mol%. Phylogenetic tree based on 16S rRNA gene sequences and concatenated amino acid sequences of 122 single-copy conserved proteins revealed a robust lineage of the strain XQ-INN 246T with members of related genera of the family Natrialbaceae . The strain possessed the polar lipids of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Based on phylogenetic analysis, phenotypic characteristics, chemotaxonomic properties and genome relatedness, the isolate was proposed as the type strain of a novel species of a new genus within the family Natrialbaceae, for which the name Salinadaptatus halalkaliphilus gen. nov., sp. nov. is proposed. The type strain is XQ-INN 246T (=CGMCC 1.16692T=JCM 33751T).

Description of Halosolutus amylolyticus gen. nov., sp. nov., Halosolutus halophilus sp. nov. and Halosolutus gelatinilyticus sp. nov., and genome-based taxonomy of genera Natribaculum and Halovarius

“…The DNA G+C content of strains LT55 T , SQT-29-1 T and WLHS5 T are 64.2, 65.0 and 65.1 %, respectively (Table S3). These values are higher than the range reported for most members of the genera Natronococcus (62.1-64.4 mol %) and Halostagnicola (59.8-60.7 mol %), but within the range of this family (59.0-69.9 mol %) [5,[30][31][32]. The 16S rRNA gene sequences of strains LT55 T , SQT-29-1 T and WLHS5 T extracted from whole-genome sequences were entirely consistent with those sequenced by conventional Sanger method.…”

Genome-based classification of the class Halobacteria and description of Haladaptataceae fam. nov. and Halorubellaceae fam. nov

“…In this study, the genera Halomicrococcus [28] and Halorussus [29] were found to be two relatives of the genus Haladaptatus and should be assigned to the family ‘ Haladaptataceae ’. The Natrialbaceae cluster contains 19 genera, Halobiforma [30], Halopiger [31], Halosolutus [20], Halostagnicola [32], Haloterrigena [33], Halovivax [34], Natrarchaeobaculum [35], Natrarchaeobius [36], Natrialba [37], Natribaculum [38], Natrinema [39], Natronobacterium [40], Natronobiforma [41], Natronococcus [40], Natronolimnohabitans [35], Natronorubrum [42], Natronolimnobius [43], Salinadaptatus [44] and Saliphagus [45]. The family Natronoarchaeaceae has been described recently within the order Halobacteriales , composed of the genera Halostella [46] , Natranaeroarchaeum [7], Natronoarchaeum [47] and Salinarchaeum …”