Saline extraction as a method of obtaining a mixture of biologically active compounds of protein nature from a porcine pancreas

Abstract

A relevant solution to the problem of processing meat industry waste in Russia is to obtain useful biologically active compounds from abundant organs. The aim of this study was to examine the effectiveness of the saline extraction as a method for extracting a mixture of promising biologically active compounds from the porcine pancreas, as well as to determine the optimal time for the process. The study consisted of extraction of the porcine pancreas with 0,9% sodium chloride solution for 5 h 30 min with further determination of the total protein concentration and proteomic profile of the samples taken throughout the process. Based on the analysis of the dependence of the total protein content in the extractant on time, the optimal extraction time was determined to be 135–150 minutes. When studying the results of electrophoresis and the data of their processing, the optimal extraction time for the targeted isolation of the low-molecular fraction of compounds was also determined to be 90 min. At the same time, 13 protein bands with a molecular weight of 52 kDa and below were found on the electropherograms. Saline should be considered applicable for obtaining extracts rich in biologically active substances, incl. hormones, enzymes and other physiologically active compounds.

Comparative study of technologies for extraction of biologically active substances from the raw material of animal origin

“…2) corresponding to the pancreas sample, which was not subjected to extraction. Compounds within the range of 52 kDa and less were found in the extracts, which were discussed in detail in our previous research [7]. It should be noted that the fractional bands in this extract are more saturated than the primary electropherogram of acid extraction (Figure 6, No.…”

“…The fraction of interest with molecular weight of less than 30 kDa in the final saline extract was researched in early works [7]. Using the UniProt database [3] the following low molecular weight compounds were presumably detected in the purified acid extract: chymotrypsin C (28.9 kDa), a member of family of chymotrypsin-like elastases 1 (28.8 kDa) and 2A (28.7 kDa), glutathione Stransferase omega-1 (27.4 kDa), kininogenase (27.2 kDa), proglucagon (21 kDa), secretin (14.6 kDa), trefoil factor 2 (13.8 kDa), somatostatin (12.7 kDa), colipase (12.1 kDa), inhibitor of serine protease of Kazal-type 4 (9.6 kDa) and 1 (6 kDa), precursor of pancreatic hormone (7.3 kDa) etc.…”

“…Saline extraction [7] was conducted in 0.9% sodium chloride solution by way of mixing of the finely chopped raw materials and an extractant in hydromodule in ratio of 1:5. The process lasted for 330 minutes.…”

“…The extraction scheme is shown below in Figure 1. Saline extraction [7] was conducted in 0.9% sodium chloride solution by way of mixing of the finely chopped raw materials and an extractant in hydromodule in ratio of 1:5. The process The point rate of extraction was determined by change in protein concentration (extracted substance) along the vector of time.…”

Biotechnological techniques for intensification of protein extraction from the porcine pancreas

“…Nevertheless, bioinformatic analysis with the use of the UniProt database [8] shows that this type of raw materials contains quite a large quantity of biologically active substances of protein nature such as pancreatic alpha-amylase, triacylglycerol lipase, phospholipase A2, proglucagon, pancreatic elastase and so on. This allows regarding the pancreas as a potential object for extracting target protein compounds with the following use in the pharmaceutical and/or food industries as well as in the modern laboratory practice [9].…”