Salinicoccus kekensis sp. nov., a novel alkaliphile and moderate halophile isolated from Keke Salt Lake in Qinghai, China

Gao, Miao; Wang, Lei; Chen, Sanfeng; Zhou, Yu‐Guang; Liu, Hong‐Can

doi:10.1007/s10482-010-9449-x

Cited by 15 publications

(6 citation statements)

References 24 publications

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“…Another putative ASR bioindicator, Modestobacter (family Geodermatophilaceae ), is commonly associated with dry environments: stone surfaces, desert biocrusts, and endolithic biofilms ( 63 , 64 ). Salinicoccus is a member of the Staphylococcaceae commonly found in high-salt environments, including desert soils ( 65 , 66 ), saline soils ( 67 ), coastal water ( 68 ), salt mines ( 69 , 70 ), salterns ( 71 ), and soda lakes ( 72 – 74 ). These potential bioindicator genera are not known to be alkaliphiles.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Keffer

Maresca

2021

mSystems

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Concrete is an extreme but common environment and is home to microbial communities adapted to alkaline, saline, and oligotrophic conditions. Microbes inside the concrete that makes up buildings or roads have received little attention despite their ubiquity and capacity to interact with the concrete. Because concrete is a composite of materials which have their own microbial communities, we hypothesized that the microbial communities of concrete reflect those of the concrete components and that these communities change as the concrete ages. Here, we used a 16S amplicon study to show how microbial communities change over 2 years of outdoor weathering in two sets of concrete cylinders, one prone to the concrete-degrading alkali-silica reaction (ASR) and the other having the risk of the ASR mitigated. After identifying and removing taxa that were likely laboratory or reagent contaminants, we found that precursor materials, particularly the large aggregate (gravel), were the probable source of ∼50 to 60% of the bacteria observed in the first cylinders from each series. Overall, community diversity decreased over 2 years, with temporarily increased diversity in warmer summer months. We found that most of the concrete microbiome was composed of Proteobacteria, Firmicutes, and Actinobacteria, although community composition changed seasonally and over multiyear time scales and was likely influenced by environmental deposition. Although the community composition between the two series was not significantly different overall, several taxa, including Arcobacter, Modestobacter, Salinicoccus, Rheinheimera, Lawsonella, and Bryobacter, appear to be associated with ASR. IMPORTANCE Concrete is the most-used building material in the world and a biologically extreme environment, with a microbiome composed of bacteria that likely come from concrete precursor materials, aerosols, and environmental deposition. These microbes, though seeded from a variety of materials, are all subject to desiccation, heating, starvation, high salinity, and very high pH. Microbes that survive and even thrive under these conditions can potentially either degrade concrete or contribute to its repair. Thus, understanding which microbes survive in concrete, under what conditions, and for how long has potential implications for biorepair of concrete. Further, methodological pipelines for analyzing concrete microbial communities can be applied to concrete from a variety of structures or with different types of damage to identify bioindicator species that can be used for structural health monitoring and service life prediction.

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Section: Discussionmentioning

confidence: 99%

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Keffer

Maresca

2021

mSystems

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“…Novel, moderately halophilic bacteria, Gracilibacillus kekensis sp. and Salinicoccus kekensis sp., have been previously isolated from this lake (Gao et al 2010;Gao et al 2012). However, the microbial structure and diversity has not been investigated here, nor did in these types of hypersaline lakes.…”

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confidence: 99%

Microbial community structure and diversity within hypersaline Keke Salt Lake environments

et al. 2017

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Keke Salt Lake is located in the Qaidamu Basin of China. It is a unique magnesium sulfate-subtype hypersaline lake that exhibits a halite domain ecosystem, yet its microbial diversity has remained unstudied. Here, the microbial community structure and diversity was investigated via high-throughput sequencing of the V3-V5 regions of 16S rRNA genes. A high diversity of operational taxonomic units was detected for Bacteria and Archaea (734 and 747, respectively), comprising 21 phyla, 43 classes, and 201 genera of Bacteria and 4 phyla, 4 classes, and 39 genera of Archaea. Salt-saturated samples were dominated by the bacterial genera Bacillus (51.52%-58.35% relative abundance), Lactococcus (9.52%-10.51%), and Oceanobacillus (8.82%-9.88%) within the Firmicutes phylum (74.81%-80.99%), contrasting with other hypersaline lakes. The dominant Archaea belonged to the Halobacteriaceae family, and in particular, the genera (with an abundance of >10% of communities) Halonotius, Halorubellus, Halapricum, Halorubrum, and Natronomonas. Additionally, we report the presence of Nanohaloarchaeota and Woesearchaeota in Qinghai-Tibet Plateau lakes, which has not been previously documented. Total salinity (especially Mg, Cl, Na, and K) mostly correlated with taxonomic distribution across samples. These results expand our understanding of microbial resource utilization within hypersaline lakes and the potential adaptations of dominant microorganisms that allow them to inhabit such environments.

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“…S. roseus (solar saltern) [1], Salinicoccus hispanicus (solar saltern) [2, 3], Salinicoccus alkaliphilus (soda lake) [4], Salinicoccus salsiraiae (salted skate) [5], Salinicoccus jeotgali (fermented seafood called ‘jeotgal’) [6], Salinicoccus kunmingensis (brine sample from a salt mine) [7], Salinicoccus luteus (desert soil) [8], Salinicoccus siamensis (fermented shrimp paste) [9], Salinicoccus iranensis (textile industry wastewater) [10], Salinicoccus halodurans (saline soil) [11], Salinicoccus albus (salt mine) [12], Salinicoccus carnicancri (crabs preserved in soy sauce) [13], Salinicoccus sesuvii (rhizosphere of Sesuvium portulacastrum ) [14], Salinicoccus qingdaonensis (seawater) [15], Salinicoccus halitifaciens (soda lake) [16], Salinicoccus sediminis (marine sediment) [17] and Salinicoccus amylolyticus (saltern) [18]. In addition, Salinicoccus salitudinis (saline soil) and Salinicoccus kekensis (salt lake) have been proposed [19, 20].…”

Section: Full-textmentioning

confidence: 99%

Salinicoccus cyprini sp. nov., isolated from the gut of mirror carp, Cyprinus carpio var. specularis

Talwar

Singh

Choksket

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel orange to pink coloured bacterial strain designated as CT19T was isolated from the gastrointestinal tract of mirror carp, Cyprinus carpio var. specularis (Lacepède, 1803) collected from the Gobind Sagar reservoir at village Lathiani, Una, Himachal Pradesh, India. Cells of the strain were found to be aerobic, Gram-stain-positive, non-motile and non-spore-forming coccoids. Based on the 16S rRNA gene sequence, the strain was closely related to Salinicoccus hispanicus J-82T (=DSM 5352T; 97.4 %), followed by S. sesuvii CC-SPL15-2T (=DSM 23267T; 96.4 %), S. amylolyticus JC304T (=KCTC 33661T; 95.6 %) and S. roseus DSM 5351T (95.4 %). Identity with all other members of the genus were <94.5 %. The draft genome of strain CT19T was assembled to 2.4 Mbp with a G+C content of 47.9 mol%. Average nucleotide identity and digital DNA–DNA hybridization values between strain CT19T and S. hispanicus J-82T were found to be 85.9 and 31.3% respectively which is far below the threshold for species delineation. Iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0, C16 : 0 and anteiso-C17 : 0 were the major cellular fatty acids of strain CT19T. Major polar lipids were diphosphatidylglycerol, phosphatidylgylcerol and an unidentified glycolipid. Respiratory quinone system was composed of menaquinone-6 and major cell wall amino acid was l-lysine. Based on phylogenomic, physiological and biochemical characteristics, strain CT19T represents a novel species of the genus Salinicoccus for which the name Salinicoccus cyprini sp. nov. is proposed. The type strain is CT19T (=KCTC 43022T =CCM 8886T=MCC 3834T).

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Salinicoccus kekensis sp. nov., a novel alkaliphile and moderate halophile isolated from Keke Salt Lake in Qinghai, China

Cited by 15 publications

References 24 publications

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

Microbial community structure and diversity within hypersaline Keke Salt Lake environments

Salinicoccus cyprini sp. nov., isolated from the gut of mirror carp, Cyprinus carpio var. specularis

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