Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov., two novel species of the family Flavobacteriaceae isolated from the South China Sea

Abstract

Strains J15B81-2T and J15B91T were isolated from a sediment sample collected from the South China Sea. Cells of both strains were observed to be rod-shaped, non-gliding, Gram-stain-negative, yellow-pigmented, facultatively anaerobic, catalase-positive, oxidase-negative and showing optimum growth at 30 °C. Strains J15B81-2T and J15B91T could tolerate up to 9 and 10 % (w/v) NaCl concentration and grow at pH 6.5–9.5 and 6.0–9.0, respectively. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.1–85.8 % ANIb and 31.5 % dDDH values calculated using whole genome sequences. Strains J15B81-2T and J15B91T shared highest 16S rRNA gene sequence similarity to Salinimicrobium xinjiangense CGMCC 1.12522T (98.4 %) and Salinimicrobium sediminis CGMCC 1.12641T (98.0 %), respectively. Among species with validly published names, S. sediminis CGMCC 1.12641T shared close genetic relatedness with strains J15B81-2T [85.1–85.3% average nucleotide identity based on blastBlast+ (ANIb) and 30.6 % digital DNA–DNA hybridization (dDDH)] and J15B91T (76.6–79.1 % ANIb and 21.5 % dDDH). The major fatty acid of strains J15B81-2T and J15B91T were identified as iso-C15 : 0 and iso-C16 : 0, respectively, and the major polar lipids of the two strains consisted of phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains J15B81-2T and J15B91T were determined to be 41.7 and 41.8 mol %, respectively. Both strains are considered to represent two novel species of the genus Salinimicrobium and the names Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov. are proposed for strains J15B81-2T (=KCTC 72867T=MCCC 1H00410T) and J15B91T (=KCTC 72869T=MCCC 1H00411T), respectively.

Salinimicrobium profundisediminis sp. nov., isolated from deep-sea sediment of the Mariana Trench

“…Consistent with the Table 2. Differential characteristics between strain MT39 T and its closely related strains Strains: 1, MT39 T (data from this study); 2, Salinimicrobium terrea CGMCC 1.6308 T (data from this study and data indicated by * from [6]); 3, Salinimicrobium xinjiangense BH206 T (data from [3] and data indicated by † from [12]); 4, Salinimicrobium sediminis JC207 T (data from [9] and data indicated by † from [12]); 5, Salinimicrobium nanhaiense J15B81-2 T (data from [12]). All strains are negative for flagellar and gliding motility and positive for catalase and hydrolysis of aesculin.…”

“…Members of Salinimicrobium are characterized as aerobic or facultatively anaerobic, rod-shaped, non-flagellated bacteria, with menaquinone-6 (MK-6) as the major respiratory quinone [3,6]. They have been isolated from various marine environments [4,[7][8][9][10][11][12] and saline soil [6,13]. The Mariana Trench is the deepest part of the world's oceans.…”

Description of Salinimicrobium tongyeongense sp. nov., isolated from seawater

“…Members of the genus have been isolated from saline environments, such as soil sediment from saline lakes, deep-sea sediment, tidal-flat sediment and saline soil. The DNA G+C content range of the members of the genus Salinimicrobium is between 40.9 and 47.5 mol% [1,[5][6][7][8][9][10][11][12].…”