Salinity-induced modulation of growth and antioxidant activity in the callus cultures of miswak (Salvadora persica)

Abstract

Miswak (Salvadora persica Linn.) is a medium-sized tree, desert facultative halophytic plant. Besides edible fruits and non-edible seed oil, the plant contains several bioactive compounds like alkaloids, tannins, saponins and sterols related to food and cosmetic industries. In the present study, physiological responses and antioxidant potential under salinity stress were investigated in callus cultures of S. persica to evaluate its use as a source of antioxidant. The callus cultures were grown on MS medium supplemented with 0.5 mg/l each of 2,4,5-T and BAP, which could be established successfully by regular subcultures of slow growing callus on this medium for several months. Increased dry weight, soluble proteins, proline, soluble carbohydrates and CAT activity were recorded under NaCl stress in comparison to control cultures. The DPPH and FRAP antioxidant activities were gradually elevated in NaCl-treated callus, whereas SOD quenching was recorded maximum at 200 mM. A significant correlation between antioxidant capacity and phenol content was observed, indicating that phenolic compounds are the major contributors to the antioxidant potential in S. persica. These findings suggest that increased salinity stress caused elevated antioxidant potentials and the plants grown in such conditions may serve as potential source of antioxidant.

Salt stress enhanced antioxidant response in callus of three halophytes (Salsola baryosma, Trianthema triquetra, Zygophyllum simplex) of Thar Desert

“…These results could be explained by the fact that salinity stress may be associated with an increase in secondary metabolites. Similarly, our previous study also suggested elevated antioxidant potentials under salt stress in callus culture of Salvadora persica (Sharma & Ramawat 2013).…”

Phoenix dactylifera L. extract: antioxidant activity and its application for green biosynthesis of Ag nanoparticles as a recyclable nanocatalyst for 4-nitrophenol reduction

“…9). The results demonstrated a linear relationship between the inhibition percentage and extract concentration in agreement with the literature [64]. This small IC 50 reflects the high antiradical activity, which can be attributed to the presence of high levels of phenols and flavonoids in the extract [65].…”

Selection, Characterizations and Somatic Embryogenesis of Malaysian Salt-tolerant Rice (Oryza sativa cv. MR219) through Callogenesis

“…Plants cells have non-enzymatic and enzymatic defense system to control over production of ROS. Activity of antioxidant enzymes correlated with concentration of salt and plants spices (Sharma and Ramawat, 2013). Generally, during oxidative stress, plants with high antioxidant enzyme activity indicated better resistance to stresses (Parida and Das, 2005).…”