Salinity is the major driver of the global eukaryotic community structure in fish-canning wastewater treatment plants

Correa‐Galeote, David; Roibás, Alba; Mosquera-Corral, Anuska; Juárez-Jiménez, B.; González‐López, Jesús; Rodelas, B.

doi:10.1016/j.jenvman.2021.112623

Cited by 13 publications

(3 citation statements)

References 75 publications

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“…Operational taxonomic units (OTUs) assigned at the 97% cut-off level with a relative abundance (RA) > 0.0001% were classified taxonomically using the 16S ribosomal database from the NCBI with the aid of the blast tool of Geneious Prime v.2019 software (Geneious, USA). Correa-Galeote et al ( 2021b ) describe the bioinformatics pipeline's full details. The 16S rRNA sequences retrieved were deposited in GeneBank under the accession numbers XXXX.…”

Section: Methodsmentioning

confidence: 99%

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

et al. 2022

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Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. Therefore, this study aimed to evaluate microbial communities' size, structure and dynamics in two AGS-SBRs fed with two different concentrations of amino acids (50 and 100 mg L−1 of both amino acids). In addition, the impact of the higher level of amino acids was also determined under an acclimatization or shock strategy. While N removal efficiency decreased with amino acids, the removal of the organic matter was generally satisfactory. Moreover, the abrupt presence of both amino acids reduced even further the removal performance of N, whereas under progressive adaptation, the removal yield was higher. Besides, excellent removal rates of cysteine and methionine elimination were found, in all stages below 80% of the influent values. Generally considered, the addition of amino acids weakly impacts the microbial communities' total abundances. On the contrary, the presence of amino acids sharply modulated the dominant bacterial structures. Furthermore, the highest amino acid concentration under the shock strategy resulted in a severe change in the structure of the microbial community. Acidovorax, Flavobacterium, Methylophilus, Stenotrophomonas and Thauera stood out as the prominent bacteria to cope with the high presence of cysteine and methionine. Hence, the AGS-SBR technology is valuable for treating influents enriched in sulphur Aa inclusively when a shock strategy was used.

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Section: Methodsmentioning

confidence: 99%

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

et al. 2022

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“…This WWTP uses a conventional activated sludge (CAS) system for nutrient removal, operating at a moderate salinity level (12.76 NaCl/L), as described in full detail by Correa-Galeote et al (2021a). The anode 6 chamber was continuously fed from the top using a peristaltic pump with synthetic wastewater simulating fish-canning wastewater (Correa-Galeote et al, 2021b) with the following composition: CH 3 COONa 3H 2 O 5.6 g L -1 (2.5 g Ac -/L), NH 4 Cl 0.38 g L -1 , MgSO 4 •7H 2 O 0.1 g L -1 , K 2 HPO 4 0.085 g L -1 , KCl 0.04 g L -1 , KH 2 PO 4 0.03 g L -1 , and NaCl 5.23 g L -1 ; pH was 7.1, and conductivity was 13.9 mS cm -1 . The total organic carbon (TOC) and NaCl concentrations in the influent were adjusted to reach a final concentration of 1 and 10 g/L, respectively, as they are representative of common TOC and NaCl values in wastewater from fish-canning industries in Galicia (Northwest of Spain) (Correa-Galeaote et al, 2021a, b).…”

Section: Design and Operation Of The Mfcmentioning

confidence: 99%

Hydraulic retention time drives changes in energy production and the anodic microbiome of a microbial fuel cell (MFC)

Castellano‐Hinojosa

Gallardo-Altamirano

Pozo

et al. 2023

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The fish-canning industry generates large quantities of wastewater that typically contains high concentrations of organic matter and salts. However, little is known about the potential valorization of this type of industrial wastewater using the microbial fuel cell (MFC) technology operated in a continuous flow mode. This study investigated the impacts of three different hydraulic retention times (HRT) on the performance, energy production, and prokaryotic and eukaryotic anodic microbiome of an MFC inoculated with activated sludge from a seafood industry and fed with synthetic wastewater that mimics fish-canning effluents. Three consecutive HRTs were studied: 1 day (HRT1), 3 days (HRT3), and 6 days (HRT6) for 30 days, 21 days, and 21 days, respectively. Voltage, current density, and power density were significantly greater at HRT1 compared to HRT3 and HRT6, whereas no differences in coulombic efficiency (CE) were detected among HRTs. Decreases in the efficiency of removal of organic compounds and increases in the abundance of archaeal communities with increased HRT was related to limited energy production at greater HRT. The increased energy production at HRT1 was tightly linked to increased and decreased absolute abundances of bacterial and archaeal communities, respectively. Variations in the HRT significantly impacted the diversity and composition of the prokaryotic community with critical impacts on energy production. The proliferation of known and diverse electroactive microorganisms, such as those belonging to the genera Geobacter, Shewanella, Arcobacter, and Clostridium, was related to increased energy production at HRT1. However, HRT3 and HRT6 enhanced the growth of archaeal methanogens (mainly Methanosarcina sp.), which negatively impacted current production. The eukaryotic community showed less sensitivity to changes in HRT and no significant impact on current production. The carbon oxygen demand and organic matter removal % increased from approximately 20% at HRT1 to almost 60% at HRT6. This study shows there is a critical balance between the HRT and prokaryotic microorganisms contributing to organic removal rate and increases and decreases in energy production in an MFC treating wastewater from the fish-canning industry and operated in a continuous mode.

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“…However, PHAs production from FOG-enriched streams often requires pretreatment operations, which would increase production costs [ 32 ]. Furthermore, fish-canning wastes are characterized by high contents of salt and organic matter, which can affect microbial communities in their composition and robustness [ 33 , 34 , 35 ]. Therefore, an optimization of the process is required.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

et al. 2022

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The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating MMCs are not well known. Therefore, the main objective of this study was to address the shifts in the size and structure of the bacterial communities in two lab-scale sequencing batch reactors (SBRs) fed with fish-canning effluents and operated under non-saline (SBR-N, 0.5 g NaCl/L) or saline (SBR-S, 10 g NaCl/L) conditions, by using a combination of quantitative PCR and Illumina sequencing of bacterial 16S rRNA genes. A double growth limitation (DGL) strategy, in which nitrogen availability was limited and uncoupled to carbon addition, strongly modulated the relative abundances of the PHA-accumulating bacteria, leading to an increase in the accumulation of PHAs, independently of the saline conditions (average 9.04 wt% and 11.69 wt%, maximum yields 22.03 wt% and 26.33% SBR-N and SBR-S, respectively). On the other hand, no correlations were found among the PHAs accumulation yields and the absolute abundances of total Bacteria, which decreased through time in the SBR-N and did not present statistical differences in the SBR-S. Acinetobacter, Calothrix, Dyella, Flavobacterium, Novosphingobium, Qipengyuania, and Tsukamurella were key PHA-accumulating genera in both SBRs under the DGL strategy, which was revealed as a successful tool to obtain a PHA-enriched MMC using fish-canning effluents.

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Salinity is the major driver of the global eukaryotic community structure in fish-canning wastewater treatment plants

Cited by 13 publications

References 75 publications

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

Hydraulic retention time drives changes in energy production and the anodic microbiome of a microbial fuel cell (MFC)

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

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