Salinity Stress Does Not Affect Root Uptake, Dissemination and Persistence of Salmonella in Sweet-basil (Ocimum basilicum)

Nirit, Bernstein; Sela, Shlomo; Dudai, Nativ; Gorbatsevich, Elena

doi:10.3389/fpls.2017.00675

Cited by 16 publications

(17 citation statements)

References 83 publications

(113 reference statements)

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“…For the analyses of Cl, dried plant samples were extracted with a dilute acid solution containing 0.1 N HNO 3 . Cl was measured by potentiometric titration (PCLM3 Jenway, Bibby Scientific Ltd., T/As Jenway, Dunmow, UK) (Bernstein et al., 2017). For the analysis of Ca, Mg, Fe, Zn, Mn, and Cu, the dry tissue was digested with HNO 3 (65%) and HClO 4 (70%), and the elements were analyzed with an atomic absorption spectrophotometer, AAnalyst 400 AA Spectrometer (PerkinElmer, Massachusetts, USA).…”

Section: Methodsmentioning

confidence: 99%

Impact of N, P, K, and Humic Acid Supplementation on the Chemical Profile of Medical Cannabis (Cannabis sativa L)

Gorelick²,

et al. 2019

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Mineral nutrition is a major factor affecting plant growth and function. Increasing evidence supports the involvement of macro and micronutrients in secondary metabolism. The use of the appropriate nutritional measures including organic fertilizers, supplements, and biostimulants is therefore a vital aspect of medicinal plant production including medical cannabis. Due to legal restriction on cannabis research, very little information is available concerning the effects of nutritional supplements on physiological and chemical properties of medical cannabis, and their potential role in standardization of the active compounds in the plant material supplied to patients. This study therefore evaluated the potential of nutritional supplementations, including humic acids (HAs) and inorganic N, P, and K to affect the cannabinoid profile throughout the plant. The plants were exposed to three enhanced nutrition treatments, compared to a commercial control treatment. The nutrition treatments were supplemented with HA, enhanced P fertilization, or enhanced NPK. The results demonstrate sensitivity of cannabinoids metabolism to mineral nutrition. The nutritional supplements affected cannabinoid content in the plants differently. These effects were location and organ specific, and varied between cannabinoids. While the P enhancement treatment did not affect THC, CBD, CBN, and CBG concentrations in the flowers from the top of the plants, a 16% reduction of THC concentration was observed in the inflorescence leaves. Enhanced NPK and HA treatments also produced organ-specific and spatially specific responses in the plant. NPK supplementation increased CBG levels in flowers by 71%, and lowered CBN levels in both flowers and inflorescence leaves by 38 and 36%, respectively. HA was found to reduce the natural spatial variability of all of the cannabinoids studied. However, the increased uniformity came at the expense of the higher levels of cannabinoids at the top of the plants, THC and CBD were reduced by 37 and 39%, respectively. Changes in mineral composition were observed in specific areas of the plants. The results demonstrate that nutritional supplements influence cannabinoid content in cannabis in an organ- and spatial-dependent manner. Most importantly, the results confirm the potential of environmental factors to regulate concentrations of individual cannabinoids in medical cannabis. The identified effects of nutrient supplementation can be further developed for chemical control and standardization in cannabis.

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Section: Methodsmentioning

confidence: 99%

Impact of N, P, K, and Humic Acid Supplementation on the Chemical Profile of Medical Cannabis (Cannabis sativa L)

Gorelick²,

et al. 2019

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“…Basil is referred to as the "king of the herbs" [50]. The essential oil of basil is used in various food products, perfumes, insecticides, medicines, and industrial products [51,52]. Basil is sensitive to high fertility [53].…”

Section: Introductionmentioning

confidence: 99%

Biochar and Vermicompost Amendments Affect Substrate Properties and Plant Growth of Basil and Tomato

Huang

Gu²,

et al. 2020

Agronomy

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The suitability of biochar (BC) as a container substrate depends on the BC mix ratio and plant species. Mixes with mixed hardwood BC (20%, 40%, 60%, and 80%, by volume) and vermicompost (VC; 5%, 10%, 15%, and 20%, by volume) were evaluated as container substrates on basil (Ocimum basilicum L.) and tomato (Solanum lycopersicum L. ‘Roma’) plants compared to a commercial peat-based substrate (CS). The CS made up the rest of the volume when BC and VC did not add up to 100%. The total porosity of all mixes with BC, VC, and CS (BC:VC:CS mixes) was similar to the control. Mixes with 80% BC had lower container capacity than the control. At 9 weeks after transplanting, the leachate pH of all the BC:VC:CS mixes was higher than that of the control, except for mixes of 20%BC and 5%VC with the rest (75%) being CS (20BC:5VC:75CS) and 20BC:10VC:70CS with tomato plants. The soil plant analysis development (SPAD) readings in BC:VC:CS mixes were similar to or higher than the control except for tomato plants in 80BC:5VC:15CS, 80BC:15VC:5CS, and 80BC:20VC:0CS mixes. Plants in BC:VC:CS mixes had similar growth indexes and total dry weight with respect to those in 100% CS, with the root DW of basil plants in 60BC:15VC:25CS being the highest among all treatments. Therefore, the BC (20%, 40%, 60%, or 80%, by volume) and VC (5%, 10%, 15%, or 20%, by volume) mixes had the potential to replace CS for container-grown plants, with the estimate wholesale price for 80BC:5VC:15CS was only 61.6% that of the control.

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“…Li and Uyttendaele (2018) examined the pathogenicity of S. enterica serovar Typhimurium (strain SL 1344) and S. enterica serovar Thompson (strains RM1987 and FMFP 899), along with two types of human noroviruses (NoV) surrogates, Murine norovirus-1 (MNV-1), and Tulane virus (TV) on O. basilicum (Figure 3F) as CFU and plague-forming unit (PFU). Effects of O. basilicum on these pathogens are discussed below: Bernstein et al (2017) inoculated soil, leaf (petiole), and leaf sap of 60-day-old O. basilicum (with and without 0-500-mM NaCl treatment) with 10 6 CFU g/soil, 10 9 CFU ml/l and 10 3 CFU ml/l suspension of S. enterica, respectively. Salmonella concentration was determined in potting mixture, shoots, leaves, and leaf sap.…”

Section: In Situ Effect Of O Basilicum On Human Pathogenmentioning

confidence: 99%

Harnessing the Potential of Roots of Traditional Power Plant: Ocimum

2021

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Genus Ocimum of Labiatae is well known in all traditional medicinal systems like Ayurveda, Unani, Siddha, and Homeopathy. The pharmaceutical activities of different species of Ocimum attributed to all plant parts. Roots are the most significant vital organ of the plant, as they absorb water and nutrients from soil and transport to aerial parts of the plants. Roots of Ocimum were found helpful with free-radical scavenging activity to improve physical and mental strength as well as to treat diabetes, malaria, and liver problems. Antibacterial activity of Ocimum roots and its main component, rosmarinic acid, is very beneficial to protect against several human pathogens, including bacteria and viruses. Being so important in every way, roots of Ocimum need healthy rhizosphere. Bacteria, fungi, nematodes, types of soil, fungicide, pesticides, salt, radioactive elements, as well as heavy metal contaminations, affect roots and overall growth of Ocimum in positive or negative ways. Each component of rhizosphere (natural, treatment or contamination) affects the roots, which highlights current ecological scenario to discover biosafe and more productive approaches. For such prestigious organ of Ocimum, development of in vitro root cultures and hairy root cultures assists to reduce the efforts and timing of the traditional cultivation process along with elimination of negative factors in rhizosphere. Different strains of Agrobacterium rhizogenes, various media compositions, as well as discrete treatments, like elicitors, on nonidentical species or cultivars of Ocimum boost the root induction, biomass, and accumulation of phytoceuticals differently. Hairy roots and in vitro roots of Ocimum accumulate higher quantity of therapeutic metabolites. These metabolites include several phenolics (like rosmarinic acid, 3-hydroxybenzoic acid, m-coumaric acid, p-coumaric acid, caffeic acid, ferulic acid, vanillic acid, chicoric acid, and lithospermic acid), triterpenes (such as betulinic acid, 3-epimaslinic acid, alphitolic acid, euscaphic acids, oleanolic acid, and ursolic acid) as well as flavonoids (flavones, flavonols, and dihydroflavonols). This review highlights pharmaceutical applications of Ocimum roots, a great deal of rhizosphere components and in vitro culturing techniques to enhance biomass as well as chief phytoceuticals.

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Salinity Stress Does Not Affect Root Uptake, Dissemination and Persistence of Salmonella in Sweet-basil (Ocimum basilicum)

Cited by 16 publications

References 83 publications

Impact of N, P, K, and Humic Acid Supplementation on the Chemical Profile of Medical Cannabis (Cannabis sativa L)

Impact of N, P, K, and Humic Acid Supplementation on the Chemical Profile of Medical Cannabis (Cannabis sativa L)

Biochar and Vermicompost Amendments Affect Substrate Properties and Plant Growth of Basil and Tomato

Harnessing the Potential of Roots of Traditional Power Plant: Ocimum

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