Saliva and Serum Protein Adsorption on Chemically Modified Silica Surfaces

Abstract

Biomaterials, once inserted in the oral cavity, become immediately covered by a layer of adsorbed proteins that consists mostly of salivary proteins but also of plasma proteins if the biomaterial is placed close to the gingival margin or if it becomes implanted into tissue and bone. It is often this protein layer, rather than the pristine biomaterial surface, that is subsequently encountered by colonizing bacteria or attaching tissue cells. Thus, to study this important initial protein adsorption from human saliva and serum and how it might be influenced through chemical modification of the biomaterial surface, we have measured the amount of protein adsorbed and analyzed the composition of the adsorbed protein layer using gel electrophoresis and western blotting. Here, we have developed an in vitro model system based on silica surfaces, chemically modified with 7 silane-based self-assembled monolayers that span a broad range of physicochemical properties, from hydrophilic to hydrophobic surfaces (water contact angles from 15° to 115°), low to high surface free energy (12 to 57 mN/m), and negative to positive surface charge (zeta potentials from –120 to +40 mV at physiologic pH). We found that the chemical surface functionalities exerted a substantial effect on the total amounts of proteins adsorbed; however, no linear correlation of the adsorbed amounts with the physicochemical surface parameters was observed. Only the adsorption behavior of a few singular protein components, from which physicochemical data are available, seems to follow physicochemical expectations. Examples are albumin in serum and lysozyme in saliva; in both, adsorption was favored on countercharged surfaces. We conclude from these findings that in complex biofluids such as saliva and serum, adsorption behavior is dominated by the overall protein-binding capacity of the surface rather than by specific physicochemical interactions of single protein entities with the surface.

Insight Into Corrosion of Dental Implants: From Biochemical Mechanisms to Designing Corrosion-Resistant Materials

“…From the moment dental implants are inserted in the oral cavity, the fate of maintaining their physicochemical properties, especially the integrity of the protective TiO 2 passive layer against corrosion, is highly dependent on the challenging conditions of the hostile electrolytic oral environment [ 8 ]. Usually, the oral fluids (i.e., saliva, blood plasma) are characterized by the presence of organic and inorganic substances in combination with a pH buffering mechanism that regulates the hypotonic and physiological condition of the medium (pH between 6 and 7) [ 15 , 16 ]; however, some factors such as microbial metabolites and corrosive substances can reduce the pH, turning the environment acidic and highly reactive to chemically attack the metallic surfaces [ 7 , 17 , 18 ].…”

Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli

“…This applies to solutions that contain hundreds of different proteins, such as human serum and saliva used in our study. 48 Lehnfeld et al 49 showed that maximum protein adsorption on chemically modified silica surfaces occurred after one hour for saliva and 10 min for the much higher concentrated human serum. However, they observed that adsorbed proteins did not correlate linearly with surface physicochemical parameters.…”

Bone Formation on Murine Cranial Bone by Injectable Cross-Linked Hyaluronic Acid Containing Nano-Hydroxyapatite and Bone Morphogenetic Protein

“…We used three mixed proteins due to their availability and cost performance. The approximate molecular weights of BMP, BSA, and collagen type I are 26, 66, and 300 kDa, respectively [58]. The size of the protein is a key element for apatite binding.…”