«Salivaomics» of Different Molecular Biological Subtypes of Breast Cancer

Bel’skaya, Lyudmila V.; Sarf, Elena A.

doi:10.3390/cimb44070211

Cited by 7 publications

(7 citation statements)

References 61 publications

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“…Another study by Bel’skaya et al . [ 63 ] found that there was no statistical difference of antioxidant activity in the different BC subtypes. According to previous literature, our results suggest that the anti-oxidative capacity is equivalent in different BC subtypes.…”

Section: Discussionmentioning

confidence: 99%

Expression profile of interleukin-6, 4-hydroxy-2-nonenal, and hypoxia-inducible factor 1-α in women with breast cancer and their association with clinicopathological parameters

Yahia,

Tahari,

Medjdoub

et al. 2023

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Introduction Breast cancer (BC) is the most common malignancy in women worldwide, representing a major public health burden. Therefore, there is a need to identify circulating biomarkers for the early detection of BC and to facilitate the diagnosis. Interleukin-6 (IL-6), 4-hydroxy-2-nonenal (4-HNE), and hypoxia-inducible factor 1-α (HIF-1α) are biomarkers involved in the initiation and progression of this disease. The aim of this study was to evaluate the concentrations of these molecules and to find a possible correlation with the clinicopathological parameters of patients with BC from western Algeria. Material and methods We evaluated the serum levels of IL-6, 4-HNE, and HIF-1α by ELISA technique and compare them in different age groups and BC molecular subtypes, and then correlated them with clinicopathological parameters. Results Our study revealed a significant increase in 4-HNE (p < 0.05), and a negative correlation (p < 0.05) was found between IL-6 serum levels and lymph node count, but not (p > 0.05) between 4-HNE, HIF-1α and lymph node count. No significant correlation (p > 0.05) was found between IL-6, 4-HNE, HIF-1α , and Scarff-Bloom-Richardson grade. Furthermore, there was no significant difference ( p > 0.05) in serum levels of IL-6, 4-HNE, and HIF-1α in the different age groups and molecular subtypes of BC. Conclusions The data obtained show that the presence of lipid peroxidation (4-HNE) is a marker of oxidative stress, and that IL-6 is a good prognostic factor due to its negative effect on the number of lymph nodes. Furthermore, age and BC molecular subtypes do not influence the serum concentrations of IL-6,4-HNE, and HIF-1α.

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Section: Discussionmentioning

confidence: 99%

Expression profile of interleukin-6, 4-hydroxy-2-nonenal, and hypoxia-inducible factor 1-α in women with breast cancer and their association with clinicopathological parameters

Yahia,

Tahari,

Medjdoub

et al. 2023

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“…This may be due to the presence in the saliva of elderly adults of under-oxidized productssubstrates of endogenous intoxication, formed, among other things, as a result of free radical processes [18]. The presence in the biological fluid of such compounds capable of acting as pronounced inhibitors of crystallization, and provides conditions for suppressing the formation of organo-mineral aggregates [18,19], described by visual assessment as crystalline elements [10].…”

Section: Methodsmentioning

confidence: 99%

Crystalloscopic analysis in detection of saliva metabolome specialties in elderly people

Martusevich,

Kashirina,

Fedotova

et al. 2024

BIO Web Conf.

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The purpose of this study was to assess the features of saliva crystallization in middle-aged and elderly people. The study included middle-aged (45-60 years old; n=63) and elderly (over 60 years old; n=78) men who signed an informed consent to participate. Crystalloscopic analysis of saliva in order to characterize the metabolic characteristics of the body and the composition of biological fluids. The intrinsic crystallization of mixed saliva was studied using the method of classical crystalloscopy. The structure index, crystallizability, facia destruction degree and the clearity of the marginal protein zone were determined. Each of the parameters was expressed on a 5-point scale. In general, the study made it possible to establish the presence of shifts in the crystallogenic properties of mixed saliva in the older age group (over 60 years old) relative to middle-aged people. Thus, the features of the crystalloscopic picture of biological fluid in elderly subjects included a decrease in the total crystallogenic potential of biological fluid, which was manifested in a decrease in crystallizability and the structural index of dehydrated saliva facies. In addition, the involutive shifts of the crystallograms were represented by an increase in the degree of destruction of facies and the expansion of the marginal zone of micropreparations. The revealed transformations of the crystallogenic properties of the biological medium allow us to speak about age-dependent characteristics of the component composition and physico-chemical parameters of saliva and, therefore, indirectly describe the involutive changes in the metabolome of this biological fluid.

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“…To develop non-invasive diagnostic tests and discover new biomarkers for BC, classic omics-based strategies have allowed significant advances in blood/plasma-based omics. Salivaomics [ 28 ], urinomics, and milkomics have appeared as integrative omics that may develop a high potential for early diagnoses in BC. Thus, an analysis of the tumor circulome is considered a novel frontier in liquid biopsies.…”

Section: Introductionmentioning

confidence: 99%

Omics-Based Investigations of Breast Cancer

Neagu,

Whitham,

Bruno

et al. 2023

Molecules

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Breast cancer (BC) is characterized by an extensive genotypic and phenotypic heterogeneity. In-depth investigations into the molecular bases of BC phenotypes, carcinogenesis, progression, and metastasis are necessary for accurate diagnoses, prognoses, and therapy assessments in predictive, precision, and personalized oncology. This review discusses both classic as well as several novel omics fields that are involved or should be used in modern BC investigations, which may be integrated as a holistic term, onco-breastomics. Rapid and recent advances in molecular profiling strategies and analytical techniques based on high-throughput sequencing and mass spectrometry (MS) development have generated large-scale multi-omics datasets, mainly emerging from the three ”big omics”, based on the central dogma of molecular biology: genomics, transcriptomics, and proteomics. Metabolomics-based approaches also reflect the dynamic response of BC cells to genetic modifications. Interactomics promotes a holistic view in BC research by constructing and characterizing protein–protein interaction (PPI) networks that provide a novel hypothesis for the pathophysiological processes involved in BC progression and subtyping. The emergence of new omics- and epiomics-based multidimensional approaches provide opportunities to gain insights into BC heterogeneity and its underlying mechanisms. The three main epiomics fields (epigenomics, epitranscriptomics, and epiproteomics) are focused on the epigenetic DNA changes, RNAs modifications, and posttranslational modifications (PTMs) affecting protein functions for an in-depth understanding of cancer cell proliferation, migration, and invasion. Novel omics fields, such as epichaperomics or epimetabolomics, could investigate the modifications in the interactome induced by stressors and provide PPI changes, as well as in metabolites, as drivers of BC-causing phenotypes. Over the last years, several proteomics-derived omics, such as matrisomics, exosomics, secretomics, kinomics, phosphoproteomics, or immunomics, provided valuable data for a deep understanding of dysregulated pathways in BC cells and their tumor microenvironment (TME) or tumor immune microenvironment (TIMW). Most of these omics datasets are still assessed individually using distinct approches and do not generate the desired and expected global-integrative knowledge with applications in clinical diagnostics. However, several hyphenated omics approaches, such as proteo-genomics, proteo-transcriptomics, and phosphoproteomics-exosomics are useful for the identification of putative BC biomarkers and therapeutic targets. To develop non-invasive diagnostic tests and to discover new biomarkers for BC, classic and novel omics-based strategies allow for significant advances in blood/plasma-based omics. Salivaomics, urinomics, and milkomics appear as integrative omics that may develop a high potential for early and non-invasive diagnoses in BC. Thus, the analysis of the tumor circulome is considered a novel frontier in liquid biopsy. Omics-based investigations have applications in BC modeling, as well as accurate BC classification and subtype characterization. The future in omics-based investigations of BC may be also focused on multi-omics single-cell analyses.

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«Salivaomics» of Different Molecular Biological Subtypes of Breast Cancer

Cited by 7 publications

References 61 publications

Expression profile of interleukin-6, 4-hydroxy-2-nonenal, and hypoxia-inducible factor 1-α in women with breast cancer and their association with clinicopathological parameters

Expression profile of interleukin-6, 4-hydroxy-2-nonenal, and hypoxia-inducible factor 1-α in women with breast cancer and their association with clinicopathological parameters

Crystalloscopic analysis in detection of saliva metabolome specialties in elderly people

Omics-Based Investigations of Breast Cancer

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