Salivary Biochemistry Associated with Helicobacter Pylori Positivity in Patients with Chronic Gastritis

Abstract

Introduction: Invasive endoscopic-biopsy techniques are most frequently used for the diagnosis and monitoring of chronic gastritis. Finding non-invasive laboratory markers would enable the patient to save money and hassle. Saliva is progressively recognized as the useful non-invasive material of diagnosis because of its critical protective role for the digestive system. Aim: The parameters of biochemical in saliva of chronic gastritis patients (HP- & HP+), comparing their concentrations to those of healthy individuals and establishing correlations between their serum and salivary concentrations with the goal of potentially using the data as the diagnostic tool. Material and method: The study was conducted at the Dept. of Medicine, Pakistan Institute of Medical Sciences, Islamabad, Pakistan. There were 140 participants in the study, 60 of them had chronic gastritis (mean age, 58.7312.08 years; 44 HP+ and 16 HP-); disease activity was determined by endoscopic, serological, and subjective symptoms. Eighty healthy, non-smoking volunteers (mean age, 56.868.67 years) made up the control group. Saliva and serum that had not been activated were analysed. Total Protein (TPro), Secretory IgA, Albumin (Alb), and Uric Acid (UA), are the variables we examine (sIg A). They are examined using pre-made B.Coulter kits and an ELISA reader from DiaMetra Italy that adapts oral fluid procedures of the Olympus biochemical analyzer. Results: In comparison to the control group, HP+ patients had significantly increased levels of sIgA (p0.0001), Alb (p0.0001), and TP (p=0.0434), but not the UA. For only the UA, we revealed the link between serum/saliva values (r=0.3389) (p=0.011). Endoscopic inflammatory alterations and UA had a moderately negative connection (r=-0.4203, p=0.016). The increased oxidative stress, changed salivary flow rate, and stomach inflammation are hypothesised to be compensated for by these modifications. Practical implication : the parameters of biochemical in saliva of the chronic gastritis patients (HP- and HP+), comparing their concentrations to those of healthy individuals, and establishing correlations between their salivary and serum concentrations in order to potentially utilization of the data as the diagnostic tool Conclusion: The data show that HP+ chronic gastritis results in considerable alterations in salivary parameters. Saliva is a biological material with some limitations, but it is a good indicator of the pathological processes occurring in the digestive tract, particularly when there is an HP+ infection. Keywords: Salivary Biochemistry; Total Protein; Chronic Gastritis; Helicobacter Pylori; HP+