Salivary chenodeoxycholic acid and its glycine-conjugate: Their determination method using LC–MS/MS and variation of their concentrations with increased saliva flow rate

Higashi, Tatsuya; Shibayama, Yujin; Ichikawa, Takuya; Ito, Koichi; Toyo’oka, Toshimasa; Shimada, Kazutake; Mitamura, Kuniko; Ikegawa, Shigeo; Chiba, Hitoshi

doi:10.1016/j.steroids.2010.01.015

Cited by 31 publications

(25 citation statements)

References 21 publications

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“…HP and PA rapidly reacted with biologically and/or clinically important carboxylic acids, e.g., chenodeoxycholic acid (CDCA), glycochenodeoxycholic acid (GCDCA), prostaglandin E2 (PGE2), 2-(β-carboxyethyl)-6-hydroxy-2,7,8-trimethylchroman (γ-CEHC), α-lipoic acid, homovanillic acid (HVA) and 5-hydroxyindole-3-acetic acid (HIAA), in the presence of 2,2′-dipyridyl disulfide (DPDS) and triphenylphosphine (TPP). 55,56 The resulting HP-and PAderivatives were highly responsive to ESI-MS operating in the positive-ion mode, and gave characteristic product ions during MS/MS, which enabled sensitive detection using selected reaction monitoring (SRM). Among the two reagents, PA has been more suitable for practical use 55 because of the higher sensitivity.…”

Section: Reagents For Amines and Carboxylic Acidsmentioning

confidence: 99%

Derivatization-based High-throughput Bioanalysis by LC-MS

Toyo’oka

2017

ANAL. SCI.

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Liquid chromatography coupled with mass spectrometry (LC-MS) is one of the most prominent analytical techniques due to its inherent selectivity and sensitivity. LC-MS is currently the first choice for high-throughput bioanalysis due to the advancements in MS instruments and the analytical software. Based on this situation, we are developing various types of derivatization reagents, including chiral reagents for MS and/or MS/MS detection. These developed reagents are adopted for the detection of biomarker candidates related to diseases. The biomarker candidates include not only achiral molecules, but also chiral ones. Although determining the already-identified chiral molecules is relative easy, it is very difficult to identify and/or determine unknown enantiomer(s) in real samples. To solve this difficulty, we proposed a new strategy to identify unknown enantiomeric biomarkers related to diseases. This review paper deals with the development of derivatization reagents for amines and carboxylic acids in LC-MS analysis and their application to bioanalysis.

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Section: Reagents For Amines and Carboxylic Acidsmentioning

confidence: 99%

Derivatization-based High-throughput Bioanalysis by LC-MS

Toyo’oka

2017

ANAL. SCI.

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“…However, they are sometimes highly polar and too hydrophilic for the separation on the reversed Chenodeoxycholic acid and glycochenodeoxycholic acid (saliva) (53).…”

Section: Aminesmentioning

confidence: 99%

Derivatization in liquid chromatography for mass spectrometric detection

Santa¹

2013

Drug Discov Ther

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“…Stimulation by these methods usually increases saliva flow by 2-5 times (basal flow rate, 0.5 mL/min versus increased flow rate, 1-3 mL/min). 4) However, some reports show that the stimulated saliva collection decreases the concentrations of some steroids, such as dehydroepiandrosterone sulfate (DHEAS), in the saliva 4,16) (see Section 4 for details). Taken together, the collection of unstimulated saliva into a collection tube without a collection device can avoid the above-mentioned pitfalls.…”

Section: Challenge Of Mass Spectrometry Toward the Elucidation Ofmentioning

confidence: 99%

Salivary Hormone Measurement Using LC/MS/MS: Specific and Patient-Friendly Tool for Assessment of Endocrine Function

Higashi

2012

Biological & Pharmaceutical Bulletin

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Saliva has recently been attracting attention as a patient-friendly available bio-fluid and an alternative to serum/plasma for hormone tests. LC coupled with atmospheric pressure ionization-MS/MS, especially electrospray ionization (ESI)-MS/MS, has been recently valued as a highly specific method in the analysis of salivary hormones. In this article, LC/ESI-MS/MS assays for salivary hormones are overviewed according to the papers that have been published during the last 5 years. Practical derivatization to enhance the detectabilities of hormones in ESI-MS/MS is also discussed, because a major disadvantage of using saliva is low hormone concentrations.

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Salivary chenodeoxycholic acid and its glycine-conjugate: Their determination method using LC–MS/MS and variation of their concentrations with increased saliva flow rate

Cited by 31 publications

References 21 publications

Derivatization-based High-throughput Bioanalysis by LC-MS

Derivatization-based High-throughput Bioanalysis by LC-MS

Derivatization in liquid chromatography for mass spectrometric detection

Salivary Hormone Measurement Using LC/MS/MS: Specific and Patient-Friendly Tool for Assessment of Endocrine Function

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