Salivary extracellular vesicles can modulate purinergic signalling in oral tissues by combined ectonucleoside triphosphate diphosphohydrolases and ecto-5′-nucleotidase activities

Extracellular vesicles regulate purinergic signaling and epithelial sodium channel expression in renal collecting duct cells

“…In that sense, the downregulation of PANX1 mRNA after the addition of EVs could be a consequence of reduced extracellular ATP levels rather than a cause. Also, our data demonstrates an ATP‐hydrolyzing activity by HCD EVs (Figure 4B), unlike HK2 EVs, which is in line with previous evidence for cancer‐derived, 22 and saliva exosomes 55 . Proteomics analysis of HK2 and HCD EVs clearly exhibited an enrichment in different subunits of the protein 14‐3‐3 in HCD vesicles.…”

“…Purinergic signaling constitutes a major regulatory mechanism involved in renal function 1,3 . Moreover, EVs from extrarenal tissues have shown to act as purinergic signaling regulators by modulating extracellular ATP levels 22,55 . Our current study constitutes the first report describing the modulation of renal purinergic signaling by EVs.…”

“…1,3 Moreover, EVs from extrarenal tissues have shown to act as purinergic signaling regulators by modulating extracellular ATP levels. 22,55 Our current study constitutes the first report describing the modulation of renal purinergic signaling by EVs. Moreover, our data, showing regulation of purinergic signaling in collecting duct cells by proximal tubule EVs, indicates the potential of EVs to mediate intersegment communication along the nephron.…”

“…Also, our data demonstrates an ATP-hydrolyzing activity by HCD EVs (Figure 4B), unlike HK2 EVs, which is in line with previous evidence for cancer-derived, 22 and saliva exosomes. 55 Proteomics analysis of HK2 and HCD EVs clearly exhibited an enrichment in different subunits of the protein 14-3-3 in HCD vesicles. Noteworthy, subunits γ, ε, and ζ have demonstrated ATPase activity.…”

Adenine-Based Purines and Related Metabolizing Enzymes: Evidence for Their Impact on Tumor Extracellular Vesicle Activities

“…Thus, it has very recently been reported the ability of different cells from normal tissues/fluids to release EVs containing purine metabolizing enzymes. This is the case of EVs isolated from rat submandibular gland or saliva, in which enzymes able to hydrolyze ATP up to ADO were identified by immune-based assays and biochemical analysis [31]. Based on preliminary results, the same authors affirm that also EVs isolated from human saliva contain the same metabolic chain.…”