Salivary flow and alpha-amylase: Collection technique, duration, and oral fluid type

Beltzer, Emilie K.; Fortunato, Christine; Guaderrama, Melissa M.; Peckins, Melissa K.; Garramone, Bianca M.; Granger, Douglas A.

doi:10.1016/j.physbeh.2010.05.016

Cited by 128 publications

(100 citation statements)

References 17 publications

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“…Since saliva collected by placing a swab underneath the tongue on yields results similar to those from whole saliva collected by passive drool, and because we were interested in assessing both cortisol and alpha-amylase from a population from which passive drool would be difficult to collect, we only used samples of saliva collected via a swab placed under the tongue for our protocol. Given the higher rates of xerostomia 3 in the older adult population and the effect of saliva flow rate on levels of salivary assays it is also important to note the time needed to collect saliva, in order to estimate the flow rate (ml/min) 21 . Multiply assay results (U/ml) by the flow rate in order to express the results as output per unit of time (U/min), to allow for comparison in data analysis.…”

Section: Discussionmentioning

confidence: 99%

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Hodgson

2013

JoVE

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Salivary measures have emerged in bio-behavioral research that are easy-to-collect, minimally invasive, and relatively inexpensive biologic markers of stress. This article we present the steps for collection and analysis of two salivary assays in research with frail, community residing older adults-salivary cortisol and salivary alpha amylase. The field of salivary bioscience is rapidly advancing and the purpose of this presentation is to provide an update on the developments for investigators interested in integrating these measures into research on aging. Strategies are presented for instructing family caregivers in collecting saliva in the home, and for conducting laboratory analyses of salivary analytes that have demonstrated feasibility, high compliance, and yield quality specimens. The protocol for sample collection includes: (1) consistent use of collection materials; (2) standardized methods that promote adherence and minimize subject burden; and (3) procedures for controlling certain confounding agents. We also provide strategies for laboratory analyses include: (1) saliva handling and processing; (2) salivary cortisol and salivary alpha amylase assay procedures; and (3) analytic considerations. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Hodgson

2013

JoVE

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“…The most common methods are: suction, fluid synthetic swab, cotton pledget, hydrocellulose microsponge or passive drooling (11). In most of these methods, after collection, salivary flow is measured by weighing the salivary sample in a graded balance.…”

Section: Introductionmentioning

confidence: 99%

Use of graduated syringes for measuring salivary flow rate: a pilot study

et al. 2010

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The evaluation of the salivary flow rate is important in several situations of dental practice. However, weighing the collected salivary volume it is not a practical method in public health services. This work evaluated the use of hypodermic graduated syringes as a simpler alternative method to measure the salivary flow rate. Saliva samples were obtained from 50 individuals (31 females and 19 males) aged 8 to 32 years (mean age: 12.4 years), recruited at the outpatient dental clinics of two public health centers. After collection, the saliva was weighed in a graduated scale and the results compared with the volume obtained through its measurement, in mL, in graduated hypodermic syringes. There was a positive and significant relationship between the two methods of measuring salivary flow rates as demonstrated by the Pearson's correlation coefficient (r = 0.996, with p<0.05). Measurement of the salivary flow rate using graduated syringes demonstrated to be a reliable method, with lower cost and more practical than the traditional method of salivary weighing.

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“…Saliva is collected with a substantial amount of bacteria and is produced by more than 20 glands operating at different rates and in different locations, which also can be influenced by diet (Beltzer et al, 2010). Thus, saliva collection requires following specific collection protocol and sample integration methods.…”

Section: Sample Extractionmentioning

confidence: 99%

“…Despite the apparent simplicity of absorbent devices, they can be affected by the spatial variation of marker concentrations within the mouth, such as in the case of α-amylase proteins (Beltzer et al, 2010), whereas the passive drool technique, although it requires following a more thorough protocol, integrates in the sample contributions from multiple locations.…”

Section: Sample Extractionmentioning

confidence: 99%

Towards autonomous lab-on-a-chip devices for cell phone biosensing

Comina

Suska

Filippini

2016

Biosensors and Bioelectronics

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Modern cell phones are a ubiquitous resource with a residual capacity to accommodate chemical sensing and biosensing capabilities. From the different approaches explored to capitalize on such resource, the use of autonomous disposable lab-on-a-chip (LOC) devices conceived as only accessories to complement cell phones underscores the possibility to entirely retain cell phones ubiquity for distributed biosensing. The technology and principles exploited for autonomous LOC devices are here selected and reviewed focusing on their potential to serve cell phone readout configurations. Together with this requirement, the central aspects of cell phones resources that determine their potential for analytical detection are examined. The conversion of these LOC concepts into universal architectures that are readable on unaccessorized phones is discussed within this context. (C) 2015 Elsevier B.V. All rights reserved.

Funding Agencies|Swedish Research Council (VR) [C0453801]; Carl Tryggers Foundation [CTS14140]

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Salivary flow and alpha-amylase: Collection technique, duration, and oral fluid type

Cited by 128 publications

References 17 publications

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Use of graduated syringes for measuring salivary flow rate: a pilot study

Towards autonomous lab-on-a-chip devices for cell phone biosensing

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