Salivary Gland Chromosomes of &lt;i&gt;Anopheles fluviatilis&lt;/i&gt; James (Diptera: Culicidae)

Sharma, Geeta; Chaudhry, S.

doi:10.1508/cytologia.41.733

Cited by 4 publications

(4 citation statements)

References 2 publications

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“…Studies carried out so far relate this aberration to the denaturing of the binding proteins due to the action of chemicals. For instance, in one of investigation, it was observed that the incidents of asynapses were more in polytene chromosomes of mosquito larvae treated with lead and mercury than in their control stocks 47 whereas according to some other research explorations, it was reported that induced asynapsis in the polytene chromosomes of Glyptotendipe barbipes occur due to polymorphism in those regions of the chromosomes which had structural heterozygosity [48][49] . Later, while studying the position of alcohol dehydrogenase genes in the polytene chromosomes of Drosophila melanogaster, it was found that different breakpoints responsible for various deficiencies arose in those chromosomes which were under replication 50 .…”

Section: Resultsmentioning

confidence: 99%

Polytene Chromosome aberrations based Genotoxicity evaluation of Dichlorvos insecticide using Drosophila malenogaster

Lovleen¹,

Kashafi²

2017

IJPTR

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: Present research work deals with evaluation of clastogenic and cytotoxic effects of Dichlorvos using larval salivary gland polytene chromosomes of Drosophila melanogaster. Exposure limit, LC 20 , was standardized by considering mortality of second instar larvae exposed to serial dilution of selected insecticide, for 24 hours. Subsequently, third instar larvae were sacrificed for temporary squash preparation of polytene chromosome, along with controls. The structural chromosomal aberration in treated stocks were incidences of ectopic pairings, paracentric inversions, chromatid breaks, chromosomal fusions, asynapses and translocations with respective value 35.7±.10.2 in treated stocks, whereas in control stocks predominant chromosomal aberrations were, paracentric inversions, chromosomal fusions and ectopic pairings with corresponding value 14.5±5.21 . Statistical analysis indicated that Dichlorvos induced significant genotoxicity in exposed larvae in comparison to control.

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Section: Resultsmentioning

confidence: 99%

Polytene Chromosome aberrations based Genotoxicity evaluation of Dichlorvos insecticide using Drosophila malenogaster

Lovleen¹,

Kashafi²

2017

IJPTR

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“…Studies carried out so far relate this aberration to the denaturing of the binding proteins due to the action of chemicals. For instance, it was observed that the incidents of asynapses were more in polytene chromosomes of mosquito larvae treated with lead and mercury than in their control stocks [ 38 ]. One more investigation conducted on mice, indicated that, The percentage of DNA breaks in testicular tissue cells in mice treated with MTX was 1.6 times higher than in the control [39] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Polytene Chromosome Aberrations Based Genotoxicity Induced by Methotrexate in Drosophila Melanogaster Oregonr

2023

IRJMETS

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Methotrexate (MTX) is a widely used drug for treating many kinds of cancer or severe and resistant forms of autoimmune diseases. However, it faces serious challenges in clinical application due to serious side effects. The objective of our present research work deals with evaluation of clastogenic and cytotoxic effects of MTX using Drosophila melanogaster Oregon R as a model organism. In our experiment exposure of MTX of different concentrations was given to Drosophila melanogaster Oregon R larvae which were sacrificed for temporary squash preparation of polytene chromosome, along with controls. The effect was observed and quantified under microscope. The structural aberration of polytene chromosomes in MTX treated stocks were observed with the incidences of asynapsis, ectopic pairing, inversion, breaks, chromosomal fusion. In control stock spontaneous aberrations were inversion, chromosomal fusion and ectopic pairing, which was significantly less compared to MTX treated groups. Subsequently, statistical analysis was done and it was concluded that MTX induced statistically significant genotoxicity in exposed larvae in comparison to control.

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“…1971;CHOWDAIAH and SEETHARAM 1975;KITZMILLER 1976;SHARMA 1976;SHARMA and CHAUDHRY 1976;SHARMA andPASAHAN 1977a, 1977b;SHARMA eta/. 1977SHARMA eta/.…”

Section: Introductionmentioning

confidence: 99%

Salivary Gland Chromosomes ofCulex Quinquefasciatus

et al. 1987

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Salivary Gland Chromosomes of <i>Anopheles fluviatilis</i> James (Diptera: Culicidae)

Cited by 4 publications

References 2 publications

Polytene Chromosome aberrations based Genotoxicity evaluation of Dichlorvos insecticide using Drosophila malenogaster

Polytene Chromosome aberrations based Genotoxicity evaluation of Dichlorvos insecticide using Drosophila malenogaster

Evaluation of Polytene Chromosome Aberrations Based Genotoxicity Induced by Methotrexate in Drosophila Melanogaster Oregonr

Salivary Gland Chromosomes ofCulex Quinquefasciatus

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