Salivary Gland Fine Needle Aspiration Using the ThinPrep® Technique

Al-Khafaji, Basim; Afify, Alaa M

doi:10.1159/000327866

Cited by 46 publications

(36 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Al-Khafaji and Afify [5] reviewed a total of 134 salivary gland FNAs, including 74 with surgical follow-up; 50 were processed by TP alone and 24 by CS. They reported that the nondiagnostic rate for TP was double that for CS (16 vs. 8%); 3 cases were falsely classified as negative on TP (2 acinic cell carcinomas and 1 low-grade mucoepidermoid carcinoma) and 1 case was falsely positive (pleomorphic adenoma diagnosed initially as carcinoma due to the presence of atypical cells without the myxoid stroma).…”

Section: Discussionmentioning

confidence: 99%

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

Rarick

Wasman²,

Michael³

2014

Acta Cytologica

View full text Add to dashboard Cite

Objective: To review the morphological features of salivary gland fine-needle aspirates (FNA) on ThinPrep® (TP) preparations. Emphasis is placed on the commonalities and specific differences between TP and conventional smear (CS) preparations. Study Design: The cytology and surgical pathology archives were searched for ‘salivary' or ‘parotid' at our institution from 2003 to 2013 for cases processed by TP and with a range of specified diagnoses and surgical follow-up. These cases were reviewed by the authors. A review of the known literature was also performed. Results: Morphological features and artifacts were noted for the most common salivary gland lesions. General features noted in the literature were identified, along with novel features identified on our review of cases. Emphasis was placed on cellular alterations, extracellular differences and architectural changes. Conclusions: The multitude of both benign and malignant lesions coupled with the unique artifacts encountered on TP makes salivary gland FNAs difficult for most practicing pathologists and cytopathologists. For this reason, many laboratories have been reluctant to implement TP preparations for these lesions. With knowledge of the specific artifacts and differences on TP compared to CS, TP can be used with confidence without a compromise in diagnostic accuracy for adequately cellular samples.

show abstract

Section: Discussionmentioning

confidence: 99%

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

Rarick

Wasman²,

Michael³

2014

Acta Cytologica

View full text Add to dashboard Cite

show abstract

“…With LBC, the monolayer preparation technique, cells can be stained better because of the absence of obscuring blood elements and air-drying artifacts which are commonly issued in SMEAR [23,24]. Since any blood cells in aspirate are lysed during preparation, excellent diagnostic accuracies have been reported in nongynecologic specimens as well as in uterine cervix [8,9,10,11,25]. Our analysis of parameters determining high cellularity in SMEAR showed that low blood contamination was the only meaningful factor other than the increased number of needle passes.…”

Section: Discussionmentioning

confidence: 99%

A Prospective Comparison of Liquid-Based Cytology and Traditional Smear Cytology in Pancreatic Endoscopic Ultrasound-Guided Fine Needle Aspiration

Lee

Choi²,

Jang³

et al. 2011

Acta Cytologica

View full text Add to dashboard Cite

Objective: To compare results of liquid-based cytology (LBC) and the conventional smear method (SMEAR) when performing endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for lesions of suspected pancreatic malignancy without an on-site cytopathologist. Study Design: Fifty-eight patients were prospectively enrolled between July and December 2009. Aspirates obtained from the first needle pass were randomized either to SMEAR or LBC. Another sample from the second needle pass was allocated to the other method. The rest of the aspirates from the third or later needle passes were used for SMEAR. Diagnostic accuracy was compared and related factors were pursued. Results: Although both methods were 100% specific, LBC was inferior to SMEAR in terms of sensitivity, negative predictive value, and accuracy. However, LBC provided correct diagnoses in 2 out of 3 cases of false negatives for malignancy by SMEAR in which blood was highly contaminated. Although no factor was identified for LBC, low blood contamination and more than 3 needle passes were related with accurate diagnosis in SMEAR. Conclusion: LBC was less accurate than SMEAR when performing pancreatic EUS-FNA without an on-site cytopathologist. However, LBC might serve as a good complement to SMEAR if blood contamination is profound.

show abstract

“…37,60,[73][74][75] Abnormalities of the chromatin pattern, such as chromatin clumping, irregularities of the nuclear membrane and nuclear molding, as well as increased nuclear to cytoplasmic ratio (N:C), loss of cellular orientation and formation of small, 3-D epithelial clusters with marked atypia have been described as major criteria of malignancy (Figure 1). 1,60,[69][70][71] Among these, chromatin clumping seems to be of the greatest predictive value. 1,60 Other features such as increased nuclear size, nuclear pleomorphism, irregular nuclear contours and nuclear grooves or macronucleoli are generally considered of minor significance and not specific enough to establish on their own a positive indication for malignancy diagnosis.…”

Section: Potential Diagnostic Pitfalls Of Brush Cytologymentioning

confidence: 99%

Value of Endoscopic Retrograde Cholangiopancreatography–Guided Brushings in Preoperative Assessment of Pancreaticobiliary Strictures

Athanassiadou

Grapsa

2008

Acta Cytologica

View full text Add to dashboard Cite

Salivary Gland Fine Needle Aspiration Using the ThinPrep® Technique

Cited by 46 publications

References 17 publications

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

The Utility of Liquid-Based Cytology in Salivary Gland Fine-Needle Aspirates: Experience of an Academic Institution

A Prospective Comparison of Liquid-Based Cytology and Traditional Smear Cytology in Pancreatic Endoscopic Ultrasound-Guided Fine Needle Aspiration

Value of Endoscopic Retrograde Cholangiopancreatography–Guided Brushings in Preoperative Assessment of Pancreaticobiliary Strictures

Contact Info

Product

Resources

About