Salivary Gland Progenitor Cells Induced by Duct Ligation Differentiate Into Hepatic and Pancreatic Lineages

Okumura, Kenji; Nakamura, Kimitoshi; Hisatomi, Yuichiro; Nagano, Koji; Tanaka, Yasuhiko; Terada, Kunihiko; Sugiyama, Toshihiro; Umeyama, Kazuhiro; Matsumoto, Kozo; Yamamoto, Tetsuro; Endo, Fumio

doi:10.1053/jhep.2003.50259

Cited by 150 publications

(128 citation statements)

References 34 publications

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“…SGP-1 cells derived from duct-ligated submandibular salivary glands can be induced to form hepatic (AFP and/or albumin positive) clusters when cultured on type-1 collagen (Okumura et al, 2003). Similarly, current work on transdifferentiation of pancreatic cells to hepatocytes (detailed below) raises the possibility that liver disease could be treated using transdifferentiated cells from the patients own pancreas.…”

Section: Therapy For Liver Diseasementioning

confidence: 99%

Transdifferentiation in developmental biology, disease, and in therapy

Thowfeequ¹,

Myatt²,

Tosh³

2007

Developmental Dynamics

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Section: Therapy For Liver Diseasementioning

confidence: 99%

Transdifferentiation in developmental biology, disease, and in therapy

Thowfeequ¹,

Myatt²,

Tosh³

2007

Developmental Dynamics

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“…[4][5][6] No salivary gland stem cell has been definitively identified as contributing to gland regeneration; however, several duct cell subtypes have been characterized as progenitor cells. [7][8][9][10][11][12] Furthermore, although the direct injection of progenitor cell populations, namely c-Kit + salivary progenitor cells 10,13 or mesenchymal stem cells (MSCs), 14 into irradiated submandibular glands (SMGs) showed some functional improvement, restoration of saliva secretion was incomplete, and highly variable. 13 To reproducibly promote regeneration and functional recovery of irradiated salivary glands, biomaterial-based approaches for cell transplantation have been explored.…”

mentioning

confidence: 99%

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

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More than 40,000 patients are diagnosed with head and neck cancers annually in the United States with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced xerostomia, but they suffer from insufficient and inconsistent gland regeneration and accompanying secretory function. To aid in the development of regenerative therapies, poly(ethylene glycol) hydrogels were investigated for the encapsulation of primary submandibular gland (SMG) cells for tissue engineering applications. Different methods of hydrogel formation and cell preparation were examined to identify cytocompatible encapsulation conditions for SMG cells. Cell viability was much higher after thiol-ene polymerizations compared with conventional methacrylate polymerizations due to reduced membrane peroxidation and intracellular reactive oxygen species formation. In addition, the formation of multicellular microspheres before encapsulation maximized cell-cell contacts and increased viability of SMG cells over 14-day culture periods. Thiol-ene hydrogel-encapsulated microspheres also promoted SMG proliferation. Lineage tracing was employed to determine the cellular composition of hydrogel-encapsulated microspheres using markers for acinar (Mist1) and duct (Keratin5) cells. Our findings indicate that both acinar and duct cell phenotypes are present throughout the 14 day culture period. However, the acinar:duct cell ratios are reduced over time, likely due to duct cell proliferation. Altogether, permissive encapsulation methods for primary SMG cells have been identified that promote cell viability, proliferation, and maintenance of differentiated salivary gland cell phenotypes, which allows for translation of this approach for salivary gland tissue engineering applications.

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“…Since the pioneer work reporting the culture of salivary epithelial cell culture (Brown 1974), several culture procedures have been described (Horie et al, 1996;Aframian et al, 2004;Joraku et al, 2005;Tran et al, 2006). A multipotent stem cell population has been discovered in human adult salivary glands (Okumura et al, 2003;Hisatomi et al, 2004;Kishi et al, 2006), but their potential for engineering salivary glands has not been proven. Developping appropriate scaffold materials will be essential for salivary gland tissue engineering (Kagami et al, 2011).…”

Section: Other Therapeutic Perspectivesmentioning

confidence: 99%