Salivary immunoglobulin concentrations in predentate and edentulous mouths

Grönblad, Eva A.; Lindholm, Kaj

doi:10.1111/j.1600-0722.1987.tb01388.x

Cited by 5 publications

(6 citation statements)

References 17 publications

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“…The effect of dentition on the concentration of immuno- globulins in the oral cavity has been shown previously to have a mixed impact on immunoblogulin levels (1,8,9). Our data indicated a significant increase in IgG concentration in edentulous or partially dentate individuals.…”

supporting

confidence: 72%

“…Physical and host factors that could affect IgG concentration or the ability to detect antibodies or antigens in OF have not been extensively investigated. The extent of dentition in an individual appears to have little to no effect on IgG in OF (1,9), but IgG variations relative to sex or age or to external factors such as smoking or chewing tobacco vary considerably among studies (2,6,(8)(9)(10)22). We recently reported the results from HIV antibody testing of a large set of OF specimens (n ϭ 4,448) (7).…”

mentioning

confidence: 99%

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Influence of Host Factors on Immunoglobulin G Concentration in Oral Fluid Specimens

Granade

Phillips

Kitson-Piggott³

et al. 2002

Clin Vaccine Immunol

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The influence of host factors (tobacco use, dentition, bleeding gums, oral rinsing, nasal medications, and time since the last meal) on immunoglobulin G (IgG) concentration in oral fluids (OF) was determined by univariate and multivariate analysis. Significant differences in IgG concentration were found to be associated with human immunodeficiency virus (HIV) status (HIV antibody positive, +16.60 microg/ml, P = 0.0001), sex (female, +1.23 microg/ml, P = 0.004), dentition (+2.83 microg/ml, edentulous versus dentulous, P = 0.0001), bleeding gums (+6.35 microg/ml, P = 0.0001), and time since the last meal (+3.55 microg/ml, >6 h, P = 0.0001). These factors could impact diagnostic methods that rely on the immunoglobulin concentration in OF specimens.

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supporting

confidence: 72%

mentioning

confidence: 99%

Influence of Host Factors on Immunoglobulin G Concentration in Oral Fluid Specimens

Granade

Phillips

Kitson-Piggott³

et al. 2002

Clin Vaccine Immunol

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“…GBI and DS correlated positively, and gingival fluid is the major source (approximately 80%) of whole saliva IgG (35). Because the amount of gingival fluid increases in inflammation, it is likely to be the source of elevated salivary IgG levels in our study.…”

Section: Discussionmentioning

confidence: 71%

Antimicrobial systems of human whole saliva in relation to dental caries, cariogenic bacteria, and gingival inflammation in young adults

Gråhn

Tenovuo

Lehtonen

et al. 1988

Acta Odontologica Scandinavica

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The association of salivary antibody (total IgA, IgG, and IgM and antibodies reactive with Streptococcus mutans) and non-antibody (lysozyme, lactoferrin, salivary peroxidase, myeloperoxidase, hypothiocyanite, thiocyanate) defense factors with oral health (past and present dental caries, gingival bleeding, the number of salivary S. mutans and lactobacilli) were studied in 50 naval recruits. Dental caries was significantly associated with large amounts of S. mutans, lactobacilli, and total salivary immunoglobulins and with low salivary flow rate and buffer capacity. Salivary anti-S. mutans antibodies did not correlate with dental caries or S. mutans levels. Moreover, none of the salivary non-antibody factors alone had any strong relationship to dental caries or S. mutans levels. Gingival inflammation was associated with elevated levels of lysozyme in whole saliva. It is concluded that in adults the associations between single-point measurements of most salivary antimicrobial constituents and the factors describing oral health are weak.

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“…Regardless of whether blood or saliva are used, selectivity in complicated biological media largely determines the accessibility of new LSPR immunoassays. Based on the potential for the onsite sampling requirement of more accessibility and safety in the future, we chose artificial saliva containing human serum albumin and multiple antibodies to mimic human saliva as a complicated sample [ 32 , 33 ]. The detection of increasing concentrations of mAb SP in mimicked human saliva (saliva buffer, S.B.)…”

Section: Resultsmentioning

confidence: 99%

Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection

Wang

Kuo

et al. 2022

J Nanobiotechnol

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Background Gold nanoparticles (AuNPs) have been widely used in local surface plasmon resonance (LSPR) immunoassays for biomolecule sensing, which is primarily based on two conventional methods: absorption spectra analysis and colorimetry. The low figure of merit (FoM) of the LSPR and high-concentration AuNP requirement restrict their limit of detection (LOD), which is approximately ng to μg mL−1 in antibody detection if there is no other signal or analyte amplification. Improvements in sensitivity have been slow in recent for a long time, and pushing the boundary of the current LOD is a great challenge of current LSPR immunoassays in biosensing. Results In this work, we developed spectral image contrast-based flow digital nanoplasmon-metry (Flow DiNM) to push the LOD boundary. Comparing the scattering image brightness of AuNPs in two neighboring wavelength bands near the LSPR peak, the peak shift signal is strongly amplified and quickly detected. Introducing digital analysis, the Flow DiNM provides an ultrahigh signal-to-noise ratio and has a lower sample volume requirement. Compared to the conventional analog LSPR immunoassay, Flow DiNM for anti-BSA detection in pure samples has an LOD as low as 1 pg mL−1 within only a 15-min detection time and 500 μL sample volume. Antibody assays against spike proteins of SARS-CoV-2 in artificial saliva that contained various proteins were also conducted to validate the detection of Flow DiNM in complicated samples. Flow DiNM shows significant discrimination in detection with an LOD of 10 pg mL−1 and a broad dynamic detection range of five orders of magnitude. Conclusion Together with the quick readout time and simple operation, this work clearly demonstrated the high sensitivity and selectivity of the developed Flow DiNM in rapid antibody detection. Spectral image contrast and digital analysis further provide a new generation of LSPR immunoassay with AuNPs. Graphical Abstract

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Salivary immunoglobulin concentrations in predentate and edentulous mouths

Cited by 5 publications

References 17 publications

Influence of Host Factors on Immunoglobulin G Concentration in Oral Fluid Specimens

Influence of Host Factors on Immunoglobulin G Concentration in Oral Fluid Specimens

Antimicrobial systems of human whole saliva in relation to dental caries, cariogenic bacteria, and gingival inflammation in young adults

Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection

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