Salivary Interleukin‐1β Levels in Patients With Chronic Periodontitis Before and After Periodontal Phase I Therapy and Healthy Controls: A Case‐Control Study

Kaushik, Rachna; Yeltiwar, Ramreddy K; Pushpanshu, Kumar

doi:10.1902/jop.2011.100472

Cited by 74 publications

(62 citation statements)

References 40 publications

(68 reference statements)

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“…In addition, the mediator panel in the Rathnayake study differed from ours, as was their statistical analysis. While we did not see a confounding effect of oral health on the associations between salivary profiles and asthma control in our study populations, others have found associations between certain inflammatory markers and oral health characteristics, including pocket depth, bleeding on probing [27], and periodontal disease status [28]–[30]. Further studies are warranted in asthmatics with a range of periodontal/gingival disease to definitively conclude that oral health does not confound associations between salivary marker profiles and asthma control.…”

Section: Discussioncontrasting

confidence: 63%

Salivary Inflammatory Mediator Profiling and Correlation to Clinical Disease Markers in Asthma

et al. 2014

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RationaleThere is a need for a readily available, non-invasive source of biomarkers that predict poor asthma control.ObjectivesWe sought to determine if there is an association between the salivary inflammatory profile and disease control in children and adults with asthma.MethodsIn this cross-sectional study, we collected demographic and clinical information from two independent populations at different sites, resulting in convenience samples of 58 pediatric and 122 adult urban asthmatics. Control was assessed by symptom questionnaire (children) and by Asthma Control Questionnaire and current exacerbation (adults). Saliva was collected in all subjects. We applied principal component analysis to a 10-plex panel of relevant inflammatory markers to characterize marker profiles and determined if profiles were associated with asthma control.ResultsThere were similar, strong correlations amongst biologically related markers in both populations: eosinophil-related: eotaxin-1/CCL11, RANTES/CCL5, and IL-5 (p<.001); myeloid/innate: IL-1β, IL-6, MCP-1/CCL2, and IL-8/CXCL8 (p<.001). The first three principal components captured ≥74% of variability across all ten analytes in both populations. In adults, the Principal Component 1 score, broadly reflective of all markers, but with greater weight given to myeloid/innate markers, was associated with Asthma Control Questionnaire score and exacerbation. The Principal Component 3 score, reflective of IP-10/CXCL10, was associated with current exacerbation. In children, the Principal Component 1, 2, and 3 scores were associated with recent asthma symptoms. The Principal Component 2 score, reflective of higher eosinophil markers, was inversely correlated with symptoms. The Principal Component 3 score was positively associated with all symptom outcomes.ConclusionThe salivary inflammatory profile is associated with disease control in children and adults with asthma.

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Section: Discussioncontrasting

confidence: 63%

Salivary Inflammatory Mediator Profiling and Correlation to Clinical Disease Markers in Asthma

et al. 2014

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“…Although it is not completely clear why two proinflammatory cytokines and a collagenase responded better to mechanical debridement than PGE 2 and MIP-1α, similar decreases in salivary concentrations of IL-1β and MMP-8 have been reported after phase I therapy in patients with chronic periodontitis. 33,42 One possible explanation is the potential role of PGE 2 in suppressing the release of IL-1β, IL-6, and TNF-α, 43,44 and these cytokines regulating the release of MMPs, which play a key role in degrading the extracellular matrix of connective tissue occurring in periodontal disease. 45 Thus, elevated levels of PGE 2 could be a protective mechanism to prevent the release of MMPs that would result in tissue breakdown.…”

Section: Discussionmentioning

confidence: 99%

Salivary Biomarkers Associated With Gingivitis and Response to Therapy

Syndergaard

Al‐Sabbagh

et al. 2014

Journal of Periodontology

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Background Salivary biomarkers are potentially important for determining the presence, risk, and progression of periodontal disease. However, clinical translation of biomarker technology from lab to chairside requires studies that identify biomarkers associated with the transitional phase between health and periodontal disease (i.e., gingivitis). Methods Eighty participants (40 with gingivitis, 40 healthy) provided saliva at baseline and 7 to 30 days later. An additional sample was collected from gingivitis participants 10 to 30 days after dental prophylaxis. Clinical parameters of gingival disease were recorded at baseline and the final visit. Salivary concentrations of interleukin (IL)-1β, IL-6, matrix metalloproteinase (MMP)-8, macrophage inflammatory protein (MIP)-1α, and prostaglandin E2 (PGE2) were measured. Results Clinical features of health and gingivitis were stable at both baseline visits. Participants with gingivitis demonstrated significantly higher bleeding on probing (BOP), plaque index (PI), and gingival index (GI) (P ≤ 0.002) and a significant drop in BOP, PI, and GI post-treatment (P ≤ 0.001). Concentrations of MIP-1α and PGE2 were significantly higher (2.8 times) in the gingivitis group than the healthy group (P ≤ 0.02). After dental prophylaxis, mean biomarker concentrations did not decrease significantly from baseline in the gingivitis group, although concentrations of IL-1β, IL-6, and MMP-8 approached healthy levels, whereas MIP-1α and PGE2 concentrations remained significantly higher than in the healthy group (P ≤ 0.04). Odds ratio analyses showed that PGE2 concentrations, alone and in combination with MIP-1α, readily discriminated gingivitis from health. Conclusions Salivary PGE2 and MIP-1α discriminate gingivitis from health, and patients with gingivitis who return to clinical health continue to produce inflammatory mediators for weeks after dental prophylaxis.

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“…These cells, including resident fibroblasts and endothelial cells, can all synthesize and release IL-1β. Thus, genetic factors, local microbial quality, individual immunologic response, and activity of different combinations of inflammatory mediators may influence IL-1β levels [29]. …”

Section: Discussionmentioning

confidence: 99%

Peripheral Blood Leukocytes Interleukin-1 Beta (IL-1β) Cytokine Hyper-Reactivity in Chronic Periodontitis

et al. 2016

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BackgroundLevels of pro-inflammatory cytokine (IL-1β) released by peripheral blood leukocyte medium (PBLM), isolated from chronic periodontitis patients (P) before therapy and matched to controls, were determined in the presence or absence of non-opsonized Escherichia coli and Staphylococcus aureus.Material/MethodsIn this investigation, 26 patients with untreated, severe, generalized, chronic periodontitis and 26 healthy subjects (H) were enrolled. Periodontal status was assessed by measuring bleeding on probing (BOP), clinical attachment loss (CAL), probing pocket depth (PPD), and Ramfjord index (PDI). The levels of IL-1β (μg/ml) were assayed by a standard Immunoenzymetric Assay Diasource IL-1β ELISA kit in PBLM.ResultsOur study showed that the values of IL-1β levels in PBLM of the P group (stimulated with non-opsonized E. coli and S. aureus) were significantly higher than in the analogous medium of H group subjects (P<0.001). All correlations between the cytokine levels of IL-1β in the samples of PBLM (stimulated with non-opsonized E. coli and S. aureus) and clinical parameters such as BOP, PPD, CAL, and PDI were significantly higher in the group of patients with periodontitis.ConclusionsLevels of IL-1β secreted by leukocytes may help measure severe, generalized, chronic periodontitis, and can be predictive of future detrimental clinical sequelae associated with chronic periodontitis.

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Salivary Interleukin‐1β Levels in Patients With Chronic Periodontitis Before and After Periodontal Phase I Therapy and Healthy Controls: A Case‐Control Study

Cited by 74 publications

References 40 publications

Salivary Inflammatory Mediator Profiling and Correlation to Clinical Disease Markers in Asthma

Salivary Inflammatory Mediator Profiling and Correlation to Clinical Disease Markers in Asthma

Salivary Biomarkers Associated With Gingivitis and Response to Therapy

Peripheral Blood Leukocytes Interleukin-1 Beta (IL-1β) Cytokine Hyper-Reactivity in Chronic Periodontitis

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