Salivary L-fucose as a biomarker for oral potentially malignant disorders and oral cancer

Sharma, Megha; Sharma, Eklavya; Prabhu, Vishnudas; Pai, Vidya; D’souza, Jyothi M. P.; Harish, Sindhu; Jose, Maji

doi:10.4103/jcrt.jcrt_552_17

Cited by 11 publications

(10 citation statements)

References 21 publications

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“…For L-proline, the concentrations were 610.1 (318.5-1244.3) µM which is about 0.070 (0.037-0.143) mg/mL. In the study of Sharma et al [46], salivary L-fucose in OPMDs and oral cancer were determined to be 7.02 mg/dl (0.0702 mg/mL) and 11.66 mg/dL (0.1166 mg/mL), respectively, while saliva from healthy controls had only 3.18 mg/dL (0.0318 mg/mL) of L-fucose. No similar study determining the concentration of L-proline in saliva was found.…”

Section: Methodsmentioning

confidence: 92%

“…We studied NIR spectra of salivary biomarkers: L-fucose [36,45,46,57,58] (L-(-)-Fucose ≥ 99%, Sigma Aldrich, Saint Louis, MO, USA) and L-proline [36,45] (L-Proline-Reagent Plus* ≥ 99%, Sigma Aldrich, Saint Louis, MO, USA), diluted into distilled water. Both substances have a solubility of 50 mg/mL in water.…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of solutions in mg/mL and µM are presented in Table 1. The aqueous solutions F1 to F3 and P1 to P3 have a higher concentration of L-fucose and L-proline than human saliva while the F4 and P4 solutions have a concentration similar to that of human saliva [45,46]. Mikkonen et al [45] determined the concentrations of L-fucose and L-proline in the saliva of healthy controls (n = 30).…”

Section: Methodsmentioning

confidence: 99%

“…Saliva consists of 94-99% of water [34,42] but it also contains biomarkers, including locally expressed proteins and substances, such as DNA, RNA, mucins, amino acids, enzymes, and primary metabolites. The concentrations of saliva biomarkers change with different diseases [20,35,[45][46][47]. Compared to blood and serum, saliva has a less complex and varying composition [20,35] Oral diseases cause abnormal changes in oral mucosal tissue.…”

Section: Near-infrared Spectroscopy In Assessing Salivary Biomarkersmentioning

confidence: 99%

See 3 more Smart Citations

Feasibility of Near-Infrared Spectroscopy for Identification of L-Fucose and L-Proline—Towards Detecting Cancer Biomarkers from Saliva

et al. 2021

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Near-infrared spectroscopy (NIRS) is a non-ionizing optical technique that can be used to quantify proteins, carbohydrates, fats, and other organic and biological substances. The aim of this study was to determine the ability of NIRS to identify different concentrations of L-fucose and L-proline solutions by utilizing different NIR spectral regions. NIR spectra of solid L-fucose and L-proline, their aqueous solutions in different concentrations, and the spectra of saliva samples collected from two patients with oral squamous cell carcinoma (OSCC) were studied. Differences in spectra of the pure solid reference samples and water were most noticeable in spectral regions 800–1250 nm and 1418–1867 nm. The saliva sample with an atypically high concentration of oral cancer biomarkers showed a similar spectral feature between 1530–1650 nm as the liquid samples with cancer biomarkers. In addition, a fine k-nearest neighbors (kNN) classifier was trained to differentiate the aqueous solutions and achieved 75.97% validation accuracy. The preliminary study presents that NIRS can be utilized to detect differences in spectra between the different biomarker concentrations in aqueous solutions. However, the qualitative measures may have resulted in limited sensitivity, which could be enhanced by additional samples and using a measurement probe dedicated to fluid measurements.

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Section: Methodsmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Near-infrared Spectroscopy In Assessing Salivary Biomarkersmentioning

confidence: 99%

See 2 more Smart Citations

Feasibility of Near-Infrared Spectroscopy for Identification of L-Fucose and L-Proline—Towards Detecting Cancer Biomarkers from Saliva

et al. 2021

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“…Almadori et al 26 suggested that salivary glutathione levels may be an index of oxidative stress at the level of the upper airways and in particular of the oral cavity and pharynx. A study by Sharma et al 61 showed a significant and gradual increase in serum and salivary L-fucose between control and oral potentially malignant disorders (OPMDs) or oral cancer (OC) samples. The authors suggested that L-fucose can be used as a reliable biomarker and saliva can be used as a diagnostic fluid for the screening and early detection of OC.…”

Section: Relations With Other Serum Biomarkersmentioning

confidence: 99%

Salivary biomarkers in oral squamous cell carcinoma

Nguyen

Sodnom-Ish

Choi

et al. 2020

JKAOMS

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In disease diagnostics and health surveillance, the use of saliva has potential because its collection is convenient and noninvasive. Over the past two decades, the development of salivary utilization for the early detection of cancer, especially oral cavity and oropharynx cancer has gained the interest of the researcher and clinician. Until recently, the oral cavity and oropharynx cancers are still having a five-year survival rate of 62%, one of the lowest in all major human cancers. More than 90% of oral cancers are oral squamous cell carcinoma (OSCC). Despite the ease of accessing the oral cavity in clinical examination, most OSCC lesions are not diagnosed in the early stage, which is suggested to be the main cause of the low survival rate. Many studies have been performed and reported more than 100 potential saliva biomarkers for OSCC. However, there are still obstacles in figuring out the reliable OSCC salivary biomarkers and the clinical application of the early diagnosis protocol. The current review article discusses the emerging issues and is hoped to raise awareness of this topic in both researchers and clinicians. We also suggested the potential salivary biomarkers that are reliable, specific, and sensitive for the early detection of OSCC.

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Harnessing aptamers for the biosensing of cell surface glycans – A review

Liu,

Zhao,

et al. 2024

Analytica Chimica Acta

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Salivary L-fucose as a biomarker for oral potentially malignant disorders and oral cancer

Cited by 11 publications

References 21 publications

Feasibility of Near-Infrared Spectroscopy for Identification of L-Fucose and L-Proline—Towards Detecting Cancer Biomarkers from Saliva

Feasibility of Near-Infrared Spectroscopy for Identification of L-Fucose and L-Proline—Towards Detecting Cancer Biomarkers from Saliva

Salivary biomarkers in oral squamous cell carcinoma

Harnessing aptamers for the biosensing of cell surface glycans – A review

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