Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats

Wang, Nannan; Liu, Zheng; Qian, Jun; Wang, Min; Li, Lili; Huang, Yuezhen; Zhang, Qian; Li, Yanfen; Yan, Fuhua

doi:10.3389/fcimb.2022.983608

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…The biological safety of biomaterials in vivo , particularly with respect to their absorption, metabolization, and bioaccumulation, always remains a crucial issue. Recently, several studies have demonstrated crosstalk between the salivary and intestinal microbiota. − To preliminarily evaluate the biosafety of Nell-1-combined AuNPs, the tissue morphology of important organs, the integrity of intestinal epithelium in colon, and the homeostasis of gut microbiome were assessed. H&E staining images of the key organs (lungs, kidneys, and liver) from different groups failed to reveal any abnormality as compared to the Ctrl group (Figure S4), suggesting that Nell-1-combined AuNPs did not exhibit any obvious organ toxicity in vivo .…”

Section: Resultsmentioning

confidence: 99%

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Liao,

Ni,

et al. 2024

ACS Appl. Mater. Interfaces

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The disruption of host–microbe homeostasis and uncontrolled inflammatory response have been considered as vital causes for developing periodontitis, subsequently leading to an imbalance between the bone and immune system and the collapse of bone homeostasis. Consequently, strategies to modulate the immune response and bone metabolization have become a promising approach to prevent and treat periodontitis. In this study, we investigated the cooperative effects of Nel-like molecule type 1 (Nell-1) and gold nanoparticles (AuNPs) on macrophage polarization, osteoclast differentiation, and the corresponding functions in an experimental model of periodontitis in rats. Nell-1-combined AuNPs in in vitro studies were found to reduce the production of inflammatory factors (TNF-α, p < 0.0001; IL-6, p = 0.0012), modulate the ratio of M2/M1 macrophages by inducing macrophage polarization into the M2 phenotype, and inhibit cell fusion, maturation, and activity of osteoclasts. Furthermore, the local application of Nell-1-combined AuNPs in in vivo studies resulted in alleviation of damages to the periodontal and bone tissues, modulation of macrophage polarization and the activity of osteoclasts, and alteration of the periodontal microbiota, in which the relative abundance of the probiotic Bifidobacterium increased (p < 0.05). These findings reveal that Nell-1-combined AuNPs could be a promising drug candidate for the prevention and treatment of periodontitis. However, Nell-1-combined AuNPs did not show organ toxicity or impair the integrity of intestinal epithelium but alter the gut microbiota, leading to the dysbiosis of gut microbiota. The adverse impact of changes in gut microbiota needs to be further investigated. Nonetheless, this study provides a novel perspective and direction for the biological safety assessment of biomaterials in oral clinical applications.

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Section: Resultsmentioning

confidence: 99%

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Liao,

Ni,

et al. 2024

ACS Appl. Mater. Interfaces

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“…Because microbial interactions between the oral cavity and gut occur frequently and the interactions may affect HTN and many other diseases ( 27 , 50 , 51 ), we analyzed communications between the oral and gut viromes. Approximately one-third of oral viruses were prevalent in the gut, and Pepyhexavirus from subgingival plaques may be transferred to the gut.…”

Section: Discussionmentioning

confidence: 99%

Alterations of oral and gut viromes in hypertension and/or periodontitis

Ye,

Zhi,

Chen

et al. 2024

mSystems

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The microbiota plays an important role in both hypertension (HTN) and periodontitis (PD), and PD exacerbates the development of HTN by oral and gut microbiota. Previous studies have focused on exploring the importance of the bacteriome in HTN and PD but overlooked the impact of the virome, which is also a member of the microbiota. We collected 180 samples of subgingival plaques, saliva, and feces from a cohort of healthy subjects (nHTNnPD), subjects with HTN (HTNnPD) or PD (PDnHTN), and subjects with both HTN and PD (HTNPD). We performed metagenomic sequencing to assess the roles of the oral and gut viromes in HTN and PD. The HTNnPD, PDnHTN, and HTNPD groups all showed significantly distinct beta diversity from the nHTNnPD group in saliva. We analyzed alterations in oral and gut viral composition in HTN and/or PD and identified significantly changed viruses in each group. Many viruses across three sites were significantly associated with blood pressure and other clinical parameters. Combined with these clinical associations, we found that Gillianvirus in subgingival plaques was negatively associated with HTN and that Torbevirus in saliva was positively associated with HTN. We found that Pepyhexavirus from subgingival plaques was indicated to be transferred to the gut. We finally evaluated viral-bacterial transkingdom interactions and found that viruses and bacteria may cooperate to affect HTN and PD. Correspondingly, HTN and PD may synergize to improve communications between viruses and bacteria. IMPORTANCE Periodontitis (PD) and hypertension (HTN) are both highly prevalent worldwide and cause serious adverse outcomes. Increasing studies have shown that PD exacerbates HTN by oral and gut microbiota. Previous studies have focused on exploring the importance of the bacteriome in HTN and PD but overlooked the impact of the virome, even though viruses are common inhabitants in humans. Alterations in oral and gut viral diversity and composition contribute to diseases. The present study, for the first time, profiled the oral and gut viromes in HTN and/or PD. We identified key indicator viruses and their clinical implications in HTN and/or PD. We also investigated interactions between viruses and bacteria. This work improved the overall understanding of the viromes in HTN and PD, providing vital insights into the role of the virome in the development of HTN and PD.

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“…Saliva contains mucus (comprised of water, lipids and proteins such as mucins) which can protect microbiota from gastric acid for survival along the gastrointestinal tract [ 15 ]. Oral gavage of periodontitis-related saliva can aggravate mice models of diabetes [ 16 ], colitis [ 17 ], Alzheimer’s disease [ 18 ] and osteoporosis [ 19 ]. Patients with severe periodontitis were estimated to swallow approximately 10 12 -10 13 Porphyromonas gingivalis (P. gingivalis) bacteria per day [ 20–22 ], and oral gavage of P. gingivalis in mice could alter gut microbiota [ 23–25 ].…”

Section: Routes Of Oral Microbiota To Affect Gut Microbiotamentioning

confidence: 99%

The interplay between oral microbiota, gut microbiota and systematic diseases

Tan

Wang²,

Gong

2023

Journal of Oral Microbiology

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Over the past two decades, the importance of microbiota in health and disease has become evident. The human gut microbiota and oral microbiota are the largest and second-largest microbiome in the human body, respectively, and they are physically connected as the oral cavity is the beginning of the digestive system. Emerging and exciting evidence has shown complex and important connections between gut microbiota and oral microbiota. The interplay of the two microbiomes may contribute to the pathological processes of many diseases, including diabetes, rheumatoid arthritis, nonalcoholic fatty liver disease, inflammatory bowel disease, pancreatic cancer, colorectal cancer, and so on. In this review, we discuss possible routes and factors of oral microbiota to affect gut microbiota, and the contribution of this interplay between oral and gut microbiota to systemic diseases. Although most studies are association studies, recently, there have been increasing mechanistic investigations. This review aims to enhance the interest in the connection between oral and gut microbiota, and shows the tangible impact of this connection on human health.

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Salivary microbiota of periodontitis aggravates bone loss in ovariectomized rats

Cited by 6 publications

References 46 publications

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis

Alterations of oral and gut viromes in hypertension and/or periodontitis

The interplay between oral microbiota, gut microbiota and systematic diseases

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