Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results

Ventura, Talita Mendes Oliveira; Santos, Karina Oliveira; Braga, Aline Silva; Grizzo, Larissa Tercília; Buzalaf, Marília Afonso Rabelo; Barbieri, Fábio Augusto; Kalva-Filho, Carlos Augusto; Faria, Murilo Henrique; Magalhães, Ana Carolina

doi:10.1007/s11332-021-00883-z

Cited by 2 publications

(15 citation statements)

References 53 publications

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“…After collection, the salivary flow was measured and the saliva samples were centrifuged at 4500 g for 15 min at 4°C to remove possible debris such as insoluble material, cells, and food debris. The supernatant from each sample was frozen at −80°C until proteomics analysis 11,12 …”

Section: Methodsmentioning

confidence: 99%

“…Data acquisition was performed by Xevo G2 QTof mass spectrometer coupled to the nanoACQUITY Ultra Performance Liquid Chromatography (both from Waters) controlled by MassLynx v.4.1 (Waters). Data collection was in data‐independent acquisition mode (LC–MSE), the mass ranges from 50 to 2000 m/z 11,12 …”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, the study by Bell et al 10 showed an increase in myofibrillar protein FSR in older men, 24 and 48 h after RT and high‐intensity interval training (HIIT), but the increase was greater in RT. These studies may indicate the potential of each exercise protocol related, for example, to phenotypic adaptations 9–12 …”

Section: Introductionmentioning

confidence: 99%

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Salivary proteomic profile of response to different resistance training protocols: A case report

Santos,

Filho,

Ventura

et al. 2024

Cell Biochemistry & Function

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Resistance training (RT) with blood flow restriction (BFR) or high intensity (HI) are effective to increase muscle mass. To understand this effect, techniques known as “omics” are used to identify possible biomarkers. This study analyzed the salivary proteomic profile of healthy individuals trained before and after two RT protocols both designed with eight exercises for upper‐ and lower‐limbs, one performed at low percentage of one‐maximum repetition (%1RM) with BFR technique, and other at high %1RM (HI) without BRF technique. Four healthy males between 18 and 28 years participated in the study. Stimulated saliva was collected before (BBFR/BHI) and immediately after (ABFR/AHI) the two RT protocols. All protein‐related processing was performed using label‐free proteomic. The difference in expression between groups was expressed as p < .05 for downregulated proteins and 1‐p > .95 for upregulated proteins. There was difference in salivary flow between ABFR and BBFR (p = .005). For HI, 87 proteins were found after the practice and 119 before. Three hemoglobin isoforms were increased in AHI compared with BHI. In the BFR comparison, 105 proteins were identified after (ABFR) and 70 before (BBFR). Among those increased ABFR, we highlight five hemoglobin isoforms and Deleted in malignant brain tumors 1 protein. Between ABFR and AHI, 17 isoforms of histones, Transaldolase, Transketolase, Glyceraldehyde‐3‐phosphate dehydrogenase, and Antileukoproteinase were decreased ABFR. For HI, there was an increase in proteins related to oxidative stress and metabolism of the musculoskeletal system, compared with BFR. HI seems to induce higher anabolic signaling to muscle mass increase and antiatherosclerotic effects.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Salivary proteomic profile of response to different resistance training protocols: A case report

Santos,

Filho,

Ventura

et al. 2024

Cell Biochemistry & Function

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“…111 In 2022, proteomics was performed in 11 studies in the SES field, demonstrating a reduced application to metabolomics which is perhaps due to transient changes in method popularity. These studies were conducted using muscle tissue, 18,96,[112][113][114][115] plasma, 116,117 saliva, 118,119 and urine. 120 Vanderboom et al 114 (Figure 7).…”

Section: Proteomicsmentioning

confidence: 99%

“…They concluded that further investigation of the muscle proteome (and transcriptome) could lead to a better understanding of the mechanisms that could limit exercise‐induced pathway responses in obese individuals. Another study similarly assessed exercise‐induced proteome changes, albeit this time in the saliva of young adults completing a 40‐min walk/rest protocol at 5‐min intervals 119 . One of the major aims of this study was to assess the differences in salivary protein content with stimulated (following chewing) or non‐stimulated collection modalities.…”

Section: Analytical Approaches For Ms‐based Investigations In Ses In ...mentioning

confidence: 99%

Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

Ong

Green

Rowland

et al. 2023

Analytical Science Advances

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Research in sport and exercise science (SES) is reliant on robust analyses of biomarker measurements to assist with the interpretation of physiological outcomes. Mass spectrometry (MS) is an analytical approach capable of highly sensitive, specific, precise, and accurate analyses of a range of biomolecules, many of which are of interest in SES including, but not limited to, endogenous metabolites, exogenously administered compounds (e.g. supplements), mineral ions, and circulating/tissue proteins. This annual review provides a summary of the applications of MS across studies investigating aspects related to sport or exercise in manuscripts published, or currently in press, in 2022. In total, 93 publications are included and categorized according to their methodologies including targeted analyses, metabolomics, lipidomics, proteomics, and isotope ratio/elemental MS. The advantageous analytical opportunities afforded by MS technologies are discussed across a selection of relevant articles. In addition, considerations for the future of MS in SES, including the need to improve the reporting of assay characteristics and validation data, are discussed, alongside the recommendation for selected current methods to be superseded by MS‐based approaches where appropriate. The review identifies that a targeted, mostly quantitative, approach is the most commonly applied MS approach within SES, although there has also been a keen interest in the use of ‘omics’ to perform hypothesis‐generating research studies. Nonetheless, MS is not commonplace in SES at this time, but its use to expand, and possibly improve, the analytical options should be continually considered to exploit the benefits of analytical chemistry in exercise/sports‐based research. Overall, it is exciting to see the gradually increasing adoption of MS in SES and it is expected that the number, and quality, of MS‐based assays in SES will increase over time, with the potential for 2023 to further establish this technique within the field.

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Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results

Cited by 2 publications

References 53 publications

Salivary proteomic profile of response to different resistance training protocols: A case report

Salivary proteomic profile of response to different resistance training protocols: A case report

Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

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