Salmon calcitonin-induced modulation of free intracellular calcium

Welch, Sandra P.; Olson, Kirsten G.

doi:10.1016/0091-3057(91)90140-w

Cited by 10 publications

(1 citation statement)

References 47 publications

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“…Moreover, it has been claimed that sCT changes the neuronal excitability in different brain regions of rats and decreased intracellular calcium by modulating synaptosomes (Twery and Moss 1985). It has also been found that sCT reduces intracellular calcium levels in response to high potassium stimulation (Welch and Olson 1991). Calcium plays a vital role in glutamate-induced cytotoxicity (Pastukhov and Borisova 2018).…”

Section: Discussionmentioning

confidence: 99%

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Taşkıran

Ergül

2021

Metab Brain Dis

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Recent evidence has shown that salmon calcitonin (sCT) has positive effects on the nervous system.However, its effect and mechanisms on glutamate-induced cytotoxicity are still unclear. The current experiment was designed to examine the effect of sCT on glutamate-induced cytotoxicity in C6 cells, involving the in ammatory and nitric oxide stress pathways. The study used the C6 glioma cell line. Four cell groups were prepared to evaluate the effect of sCT on glutamate-induced cytotoxicity. The control group was without any treatment. Cells in the glutamate group were treated with 10 mM glutamate for 24 h. Cells in the sCT group were treated with various concentrations (3, 6, 12, 25, and 50 µg/mL) of sCT for 24 hours. Cells in the sCT + glutamate group were pre-treated with various concentrations of sCT for 1 hour and then exposed to glutamate for 24 hours. The cell viability was evaluated with an XTT assay. Nuclear factor kappa b (NF-kB), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), neuronal nitric oxide synthase (nNOS), nitric oxide (NO), cyclic guanosine monophosphate (cGMP), caspase-3, and caspase-9 levels in the cells were measured by ELISA kits. Apoptosis was detected by ow cytometry method. sCT at all concentrations signi cantly improved the cell viability in C6 cells after glutamateinduced cytotoxicity (p < 0.001). Moreover, sCT signi cantly reduced the levels of NF-kB (p < 0.001), TNFα, and IL-6 levels (p < 0.001). sCT also decreased nNOS, NO, and cGMP levels (P < 0.001). Furthermore, it decreased the apoptosis rate and increased the live-cell rate in the ow cytometry (P < 0.001). In conclusion, sCT has protective effects on glutamate-induced cytotoxicity in C6 glial cells by inhibiting in ammatory and nitric oxide pathways. sCT could be a useful supportive agent for people with neurodegenerative symptoms.

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Section: Discussionmentioning

confidence: 99%

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Taşkıran

Ergül

2021

Metab Brain Dis

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Calcitonin Potentiates Oxygen–Glucose Deprivation-Induced Neuronal Death

Asrari

Lobner

2001

Experimental Neurology

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Calcitonin and its antinociceptive activity: Animal and human investigations 1975–1992

1994

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Calcitonin (CT) is a polypeptide hormone produced in the thyroid gland that regulates, blood calcium levels and bone calcium metabolism. The unexpected finding of binding sites for calcitonin in several areas of the brain oriented attention to activities of CT in the central nervous system and also to its antinociceptive action. The first report of this last effect was in 1975, and the many different experimental and clinical data on this topic reported since then are reviewed here. The heterogenous findings have been organized according to the logical classification of animal and human studies. For each of these headings, subheadings such as acute and chronic pain, different kinds of administration and different procedures used to record the results, are considered. The several proposed mechanisms of action, involving serotoninergic, catecholaminergic, Ca2+ fluxes, protein phosphorylation, beta-endorphin production, cyclooxygenase inhibition and histamine interference are also reviewed. Calcitonin, neurotensin, substance P, VIP and, recently, CGRP are some of the non-opioid peptides that have been reported to interfere with pain and that open up a new, alternative way of investigating antinociceptive drugs different than opioid or opioid-like agents. An examination of the state-of-investigation of calcitonin's antinociceptive activity in the last 17 years shows that many experimental studies indicate the existence of this effect, including studies in humans, and this opens up perspectives for therapy with a new class of antinociceptive agents.

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Salmon calcitonin-induced modulation of free intracellular calcium

Cited by 10 publications

References 47 publications

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Calcitonin Potentiates Oxygen–Glucose Deprivation-Induced Neuronal Death

Calcitonin and its antinociceptive activity: Animal and human investigations 1975–1992

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