Salmonella enteritidis in Italy

Fantasia, M.; Filetici, Emma

doi:10.1016/0168-1605(94)90194-5

Cited by 66 publications

(27 citation statements)

References 4 publications

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“…Previous studies reported that S. Enteritidis has become the main cause of salmonellosis outbreaks (9,10,12,23,30,31) and this serovar has been also the most prevalent among poultry-related samples in Brazil (32,37). Furthermore, a high adaptation of S. Enteritidis to colonize the chicken oviduct has been proposed, indicating that poultry may be an important reservoir of this pathogen (20).…”

Section: Discussionmentioning

confidence: 99%

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

Mürmann

Santos

Longaray³

et al. 2008

Braz. J. Microbiol.

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Data concerning the prevalence and populations of Salmonella in foods implicated in outbreaks may be important to the development of quantitative microbial risk assessments of individual food products. In this sense, the objective of the present study was to assess the amount of Salmonella sp. in different foods implicated in foodborne outbreaks in Rio Grande do Sul occurred in 2005 and to characterize the isolated strains using phenotypic and genotypic methods. Nineteen food samples involved in ten foodborne outbreaks occurred in 2005, and positive on Salmonella isolation at the Central Laboratory of the Health Department of the State of Rio Grande do Sul, were included in this study. Food samples were submitted to estimation of Salmonella using the Most Probable Number (MPN) technique. Moreover, one confirmed Salmonella colony of each food sample was serotyped, characterized by its XbaI-macrorestriction profile, and submitted to antimicrobial resistance testing. Foods containing eggs, mayonnaise or chicken were contaminated with Salmonella in eight outbreaks. Higher counts (>10 7 MPN.g -1) of Salmonella were detected mostly in foods containing mayonnaise. The isolation of Salmonella from multiple food items in five outbreaks probably resulted from the cross-contamination, and the high Salmonella counts detected in almost all analyzed samples probably resulted from storing in inadequate temperature. All strains were identified as S. Enteritidis, and presented a unique macrorestriction profile, demonstrating the predominance of one clonal group in foods involved in the salmonellosis outbreaks. A low frequency of antimicrobial resistant S. Enteritidis strains was observed and nalidixic acid was the only resistance marker detected.

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Section: Discussionmentioning

confidence: 99%

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

Mürmann

Santos

Longaray³

et al. 2008

Braz. J. Microbiol.

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“…There has been a dramatic increase in the incidence of Salmonella Enteritidis worldwide since 1980 (3,14,25,28). In Brazil, the increase in the incidence of S. Enteritidis from foodborne outbreaks, human infections, non-human sources, broiler carcasses and other poultry materials has been reported since the 90's (5,6,25,29,33,34), and poultry is likely the main source of human infections (10).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Oliveira¹,

Bessa²,

Santos³

et al. 2007

Braz. J. Microbiol.

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In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types.

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“…S. enteritidis is one of the primary causes of human food poisoning throughout the world, and has become a pointed public health problem [1][2][3][4] . China is the biggest country in the raising and consumption of duck in the world, but the S. enteritidis bacillus infection is a severely important infectious disease in the duck industry [5] .…”

Section: Introductionmentioning

confidence: 99%

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Yan

Cheng²,

Wang³

et al. 2008

WJG

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CONCLUSION:IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis . We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium. INTRODUCTIONSalmonella enteritidis (S. enteritidis) is the infectious disease causing Zoonoses. S. enteritidis is one of the primary causes of human food poisoning throughout the world, and has become a pointed public health problem [1][2][3][4] . China is the biggest country in the raising and consumption of duck in the world, but the S. enteritidis bacillus infection is a severely important infectious disease in the duck industry [5] . S. enteritidis outbreaks have been found to be associated with the consumption of contaminated and undercooked poultry products, such as eggs and egg-containing products, and have become a serious economic and public health problem [6] . Conventional methods for isolation of S. enteritidis are too laborious, not sufficiently sensitive or correct [7,8] . To our knowledge, a serum method for detection and antigen location of S. enteritidis has not been reported. Thus, to establish a rapid, sensitive and highly specific in paraffin slices and antigen location in infected duck tissues. METHODS:Rabbits were immunized with purified bacillus to obtain S. enteritidis -specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. S. enteritidis was detected in each organ tissue of ducklings experimentally infected with S. enteritidis . RESULTS:The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen was mainly distributed in the infected cell cytoplasm.

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Salmonella enteritidis in Italy

Cited by 66 publications

References 4 publications

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

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