Salmonella Heidelberg reduces nitrergic neurons in the myenteric plexus of the duodenum of broilers

Patricia, Franco Goncalves Previato do Amaral; Gabriela, Rocha Santos; Tatiana, Kaori Urano; Karen, da Silva Diniz; Tatiane, dos Santos Aparecido Goncalves; Grazielli, de Fatima Serenini; Luciana, Kazue Otutumi; Ricardo, de Melo Germano

doi:10.5897/ajmr2017.8593

Cited by 4 publications

(5 citation statements)

References 25 publications

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“…Studies on other bird species, such as the one by PREVIAto Do AMARAl et al (2017), found a density of 24.38±4.97 NADPH-d+/mm 2 neurons in the duodenum of broiler chickens aged 21 days. YANG et al (2013) reported 45.92±17.51 NADPH-d+/mm 2 neurons in the jejunum of broiler chickens aged 40 days.…”

Section: Resultsmentioning

confidence: 92%

Location, distribution, and quantification of myenteric plexus neurons of the jejunum of quails fed with different levels of commercial Macleaya cordata extract

et al. 2021

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Coturniculture has been promising, progressing from a subsistence to a technical activity due to its quick production, low breeding investment, and rapid economic return. After the restriction of antimicrobials as growth promoters, some studies aimed to evaluate alternative products that would make the farming of healthy birds viable without impacting their performance, with commercial Macleaya cordata extract being one of these substitutes. The functions of the gastrointestinal tract are coordinated mainly by the enteric nervous system, and the myenteric plexus is responsible for the reflex control of contractile activities of the external muscles. Thus, this study located and demonstrated the distribution of the myenteric plexus, quantifing the total population of myenteric neurons (Giemsa+) and the subpopulation of myenteric nitrergic neurons (NADPH-d+), and evaluated the effects of commercial Macleaya cordata extract on these populations of quail jejunum neurons. A total of 240 one-day-old female laying quails were distributed into four treatments, with four repetitions of 15 birds each. The test groups (T1, T2, and T3) were treated with commercial Macleaya cordata extract throughout the experimental period using the following doses: T1 - test group, basal diet added with 150 ppm of the extract in the feed; T2- test group, basal diet added with100 ppm of the extract in the feed; T3 - test group, basal diet added with 50 ppm of the extract in the feed; and T4 - control group, basal diet with no added extract. The study included histological analysis, Giemsa+, and NADPH-d+ myenteric neuron staining. The results showed that the myenteric plexus is located between longitudinal layer fibers and in the transition region between the longitudinal and circular layers of the muscular tunic, with the myenteric population organized into ganglia and isolated in the region of neuronal fiber bundles. The commercial Macleaya cordata extract showed no quantitative changes in the myenteric Giemsa+ population and myenteric NADPH-d+ subpopulation, however, the groups that consumed the extract showed greater NADPH-d+ neuron activity compared to the control group, implying that the food remained longer in the intestinal lumen, therefore, enabling greater nutrient use and resulting in increased productive performance.

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Section: Resultsmentioning

confidence: 92%

Location, distribution, and quantification of myenteric plexus neurons of the jejunum of quails fed with different levels of commercial Macleaya cordata extract

et al. 2021

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“…It is important to emphasize that the location of the myenteric plexus, as well as the shape and number of ganglia, studied in animals other than bats, showed that they may vary according to the species and segment of the digestive tract under study COSTA, 1980), in addition to eating habits (STABILLE et al, 2002;MUNNICH;GABEL;PFANNKUCHE, 2008;PREVIATO DO AMARAL et al, 2017). Furness and Costa (1980) emphasize that most of the nervous cell bodies of the enteric nervous system are found in the ganglia of the myenteric plexus.…”

Section: Resultsmentioning

confidence: 99%

Location and characterization of the myenteric plexus of insectivorous bats of the species Molossus rufus

Gerônimo

Favetta

Trindade

et al. 2023

PRW

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Bats, from the Molossidae family, are found on all continents. Molossus rufus, are found in Brazil, having an insectivorous feeding habit. The present study aimed to characterize and locate the myenteric plexus in the gastrointestinal tract of Molossus rufus bats from the northwest region of the state of Paraná. Necropsy of five male specimens was performed and the structures corresponding to the stomach and intestines were fixed in 10% neutral buffered formalin for histological preparation of the material, and 0.5cm of each segment was followed for identification of myenteric ganglia using the technique of Giemsa and the histochemistry of NADPH-diaphorase. The myenteric plexus is found between the circular and longitudinal strata of the tunica muscularis, with neurons gathered in ganglia surrounded by bundles of collagen fibers. The ganglia observed in the membrane preparations were formed by different numbers of neurons, interconnected by bundles of nerve fibers, where isolated neurons could be observed. The location of the myenteric plexus found in bats of the species Molossus rufus is similar to those found in other mammals.

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“…Characteristics of the ganglionic arrangement of myenteric plexus are described in several studies (Sant' Ana et al 1997, Yang et al 2013) that mention differences in this arrangement depending on the intestinal segment and the species studied (Irwin 1931, Furness & Costa 1980, Gabella 1990, Furness 2006 as well as in different food habits (Stabille et al 2002, Münnich et al 2008, Previato do Amaral et al 2017.…”

Section: Discussionmentioning

confidence: 99%

Quantification of the neurons of myenteric plexus of the bat molossus rufus

et al. 2020

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There are no studies that characterize the enteric nervous system (ENS) bats. The organization and density of myenteric neurons may vary according to the animal species, as well as the segment of the digestive tube considered. The nitric oxide is one of the key neurotransmitters present in the myenteric neurons, acting as a mediator in the smooth muscle relaxation. These neurons are evidenced by immunohistochemistry of nitric oxide synthase (NOS) or by NADPH-diaphorase histochemistry. In this sense, this study aimed to characterize the total neuronal population and subpopulation NADPH-d+ of the myenteric plexus present in the jejunum of the insectivore species Molossus rufus quantitatively. Five specimens were collected of M. rufus in a buffer area of the “Reserva Biológica das Perobas” in the microregion of Cianorte/PR. After the euthanasia, in a chamber saturated with isoflurane, segments were collected from the small intestine corresponding to the jejunum intended for two techniques for neuronal marking, Giemsa and NADPH-diaphorase, and a fragment to the histological technique of hematoxylin-eosin and Masson’s trichrome. All the procedures were approved by the “Comitê de Ética no Uso de Animais Unipar” (CEUA - protocol No. 34347/2017) and the “Instituto Chico Mendes de Conservação da Biodiversidade” (ICMBio - protocol No. 60061-1) The histological sections allowed to highlight the location of the myenteric plexus between the longitudinal and circular layers of the muscular tunic. The myenteric plexus had an average of total neuronal population (neurons Giemsa+) of 279.23 neurons/mm2, being the nitrergic neurons (neurons NADPH-d+) represented 20.4% of this total population, with an average of 58.14 neuron/mm2. Therefore, the collected data are consistent with previous studies in other mammalian species concerning the location of the myenteric plexus, as well as the neural myenteric proportion NADPH-d+ compared with the population of neurons Giemsa+. The gaps in the knowledge of ENS of bats limits comparative intraspecific and interspecific studies.

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Salmonella Heidelberg reduces nitrergic neurons in the myenteric plexus of the duodenum of broilers

Cited by 4 publications

References 25 publications

Location, distribution, and quantification of myenteric plexus neurons of the jejunum of quails fed with different levels of commercial Macleaya cordata extract

Location, distribution, and quantification of myenteric plexus neurons of the jejunum of quails fed with different levels of commercial Macleaya cordata extract

Location and characterization of the myenteric plexus of insectivorous bats of the species Molossus rufus

Quantification of the neurons of myenteric plexus of the bat molossus rufus

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