Salmonella prevalence and serovar distribution in healthy slaughter sheep and cattle determined by ISO 6579 and VIDAS UP Salmonella methods

Çetin, Ece; Temelli, Seran; Eyigör, Ayşegül

doi:10.1007/s13197-019-04002-2

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…The prevalence of Salmonella in cattle found in this study (3%) is consistent with the data found in the literature for Europe, where asymptomatic carriage of Salmonella in cattle is generally less than 5% based on fecal samples [8,31]. It is possible that the incidence of salmonellosis was underestimated, since fecal samples are not necessarily the most sensitive source to detect the presence of Salmonella, and some positive cattle may have been missed by the detection method used [8,17,32]. In addition, prevalence may be underestimated because the animals sampled at the slaughterhouse are healthy, which does not take into account symptomatic cattle carrying Salmonella.…”

Section: Discussionsupporting

confidence: 88%

“…In addition, prevalence may be underestimated because the animals sampled at the slaughterhouse are healthy, which does not take into account symptomatic cattle carrying Salmonella. The prevalence of Salmonella in apparently healthy cattle is of significant concern to public health [17], especially that they are able to carry more than 700 CFU per gram of Salmonella, as shown in this study, and thus may cross-contaminate the meat products during processing.…”

Section: Discussionmentioning

confidence: 75%

“…Moreover, prevalence estimates vary from 2% in Europe to 16% in North America [12][13][14]. Studies have reported 5% in Africa, 4% in Turkey and 2% in Iran [15][16][17]. Fossler et al showed the presence of Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of Salmonella in the Cattle Production in France

et al. 2021

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Salmonella is among the most common foodborne pathogens worldwide, and can lead to acute gastroenteritis. Along with poultry, cattle production is recognized as an important source of human infection. Salmonella transmission from cattle to humans can occur through the environment, or through close contact with sick animals or their derived products. This study aimed to investigate the intestinal carriage of Salmonella spp. within French cattle production. A total of 959 cattle intestinal samples, from one of the largest French slaughterhouses, were analyzed. Isolated strains were genotyped by pulsed field gel electrophoresis (PFGE), and a sub-selection was taken by whole genome sequencing (WGS). Twenty-nine samples were positive for Salmonella spp., yielding an estimated prevalence of 3% in cattle production. Eight different Salmonella serotypes were found: Montevideo was the most prevalent (34%), followed by Mbandaka (24%) and Anatum (14%). PFGE genotyping allowed the clustering of Salmonella isolates according to their serotype. Within the clusters, some isolates presented 100% similarity. To investigate potential epidemiological links between them, WGS and core genome multilocus sequence typing (cgMLST) were used, revealing identical profiles between isolates originating from different areas and/or different animal breeds. This investigation provides new insights on Salmonella serotype epidemiology in cattle production in France.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 75%

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Occurrence of Salmonella in the Cattle Production in France

et al. 2021

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“…Information obtained from various databases indicated that few reports on sensitivity studies following ISO 16140-2: 2016 have focused on L. monocytogenes or Salmonella spp. The authors of [ 15 ] evaluated the effectiveness of ISO 6579 and VIDAS UP Salmonella SPT in slaughtered sheep, reporting values of 94.44% for SE alt and 99.79% for RT. Relatively high values of SE alt and RT were reported by [ 17 ] who evaluated RT-PCR to detect L. monocytogenes contaminated in dry-cured ham.…”

Section: Discussionmentioning

confidence: 99%

“…Such international standards are used to prove that the performance characteristics of a newly developed method are at least equivalent or better than those of a standard method. In addition, few publications have reported on ISO 16140-2: 2016-based validations of different alternative methods to detect only single target bacterium in various food samples [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats

Jaroenporn

Supawasit

Bundidamorn

et al. 2022

Foods

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The aim of the study was to perform in-house validation of the developed multiplex PCR (mPCR)-based alternative method to detect Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw meats following the ISO 16140-2: 2016. A comparative study of the developed mPCR against the Bacteriological Analytical Manual (BAM) method was evaluated for inclusivity and exclusivity, sensitivity and the relative level of detection (RLOD). Inclusivity levels for each target bacterium were all 100%, while exclusivity for non-target bacteria was 100%. The sensitivity of the developed mPCR was calculated based on the analysis of 72 samples of raw meat. The sensitivity of the developed mPCR was 100%. The RLOD values of the developed mPCR for STEC, L. monocytogenes and Salmonella spp. were 0.756, 1.170 and 1.000, respectively. The developed mPCR showed potential as a tool for the fast, specific and sensitive detection of the three bacteria in the raw meat industry

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