Salmonella spp. in Chicken: Prevalence, Antimicrobial Resistance, and Detection Methods

Tan, Si Jie; Nordin, Syamilah; Effarizah, Mohd Esah; Mahror, Norlia

doi:10.3390/microbiolres13040050

Cited by 21 publications

(8 citation statements)

References 65 publications

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“…The rate of S. Enteritidis isolation was (18.60%) from the total collected samples (24/129), The rate of isolation accepted with that calculated by (Elkenany et al, 2019) (11.4 %) and (Wang et al, 2020) (16.6%). On the over mined, the high percentage of S. Enteritidis isolation was determined by (Tan et al, 2022) and (Shen et al, 2023), which was (39.7%) and (43.52%) respectively. The prevalence of salmonellosis in liver was (20%).…”

Section: Discussionmentioning

confidence: 98%

Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Hamad,

El-hofy,

Hamouda

et al. 2023

Benha Veterinary Medical Journal

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Salmonella is one of the genus under the family Enterobacteriaceae and is recorded as an important zoonotic pathogen. Salmonella infection (salmonellosis) is a serious problem for chicken farms in different areas because the broiler represents its important host. Salmonella Enteritidis (S. Enteritidis) strain affected mainly the internal organs of the chicken, resulting in elevation of the morbidity and mortality rates, consequently a high economic loss in broiler production. The aim of this work was the detection of the S. Enteritidis (sefA) gene isolated from internal organs of healthy slaughtered broiler chickens. The total samples, 129, were determined as follows: (Liver, kidneys, small intestine, gizzard, and heart blood) are (20,19, 37,30,23) respectively. The samples were collected under microbiological examination. The incidence of Salmonella Species was tabulated as flow: (20%, 10.52%, 37.83%, 6.66%, and 8.69%) from the liver, kidneys, small intestine, gizzard, and heart blood, respectively. Only eight isolates from 24 isolated strains were undergone serological tests, recording one serotype of S. Enteritidis. The high prevalence occurred in the small intestine and liver of apparently healthy broilers, and the lower prevalence occurred in the heart blood, kidneys, and gizzard of internal organs of broilers. The data resulted from antimicrobial sensitivity test application, high resistance rate to vancomycin (64.6%), gentamicin (64.6%); tetracycline (92.8%); chloramphenicol (85.7%); ciprofloxacin (35.7%); levofloxacin, (64.28%); amoxicillin + clavulanic, (100%); streptomycin, (92.85); trimethoprim + sulfamethoxaole (85.7%). While the sensitivity to florfenicol was 100%. Confirmatory PCR technique for detection of (sefA) gene amplification at (310 bp) in 8 isolated strains.

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Section: Discussionmentioning

confidence: 98%

Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Hamad,

El-hofy,

Hamouda

et al. 2023

Benha Veterinary Medical Journal

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“…This may be due to the survival of the bacteria in the skin crypts. As a common opinion, research on the prevalence of Salmonella and serovars is essential to ensuring food safety in chickens and poultry products as they are widely consumed worldwide (Tan et al ., 2022).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of Salmonella in poultry meat and products in Türkiye: Prevalence, antibiotic susceptibility and genomic characterisation

Dishan,

Hizlisoy,

Onmaz

et al. 2024

Int J of Food Sci Tech

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SummaryThe study aimed to determine the prevalence and characteristics of Salmonella isolated from raw chicken meat and products. For this purpose, a total of 293 samples were collected, including chicken breast (n = 90), skinned drumstick (n = 80), skinned chicken chop (n = 42), wing (n = 32), chicken offal (n = 27) and chicken patty (n = 22). The samples were subjected to Salmonella enterica. detection and the obtained suspicious isolates were confirmed by conventional PCR. Their phenotypical antibiotic resistance profiles were subsequently determined. The prevalence of Salmonella Enteritidis and Typhimurium serovars among S. enterica isolates were investigated using TaqMan probe Real‐Time PCR (qPCR) analysis, and the detected serovars were evaluated with whole genome sequencing. In the study, 112 (38.22%) of the 293 chicken samples contained S. enterica, with five (4.46%) and one (0.89%) of the isolates identified as Salmonella Enteritidis and Typhimurium, respectively. Antibiotic resistance analysis revealed that all isolates were sensitive to Meropenem and Aztreonam, while the most resistant antibiotics were Doxycycline (96.42%) and Trimethoprim‐sulfamethoxazole (71.42%). Whole genome sequencing, specifically SNP‐based phylogenetic analyses, indicated that Salmonella Enteritidis and Typhimurium isolates were distinct clones. All Salmonella Enteritidis isolates shared the same antigenic profiles (9: g, m:‐) and cgMLST types of 11, while the Salmonella Typhimurium isolate had cgMLST type 19 and a 4:i:1,2 antigenic profile. It was observed that the phenotypic resistance profiles of the isolates were consistent with the whole genome characterisation. The data obtained in the study reveal the continued importance of Salmonella monitoring for the poultry industry across different regions of Türkiye to maintain food safety. Chicken meat and products are indispensable to public health in providing healthy nutrition and access to animal protein. The microbiological and epidemiological risks observed in mass production can be minimised, particularly by integrating epidemiological and molecular findings with an effective strategy.

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“…Salmonella frequency in chicken and poultry products varies between fresh markets and supermarkets [11]. However, the contamination or transfer of salmonellosis usually occur primarily at the farm level, during transportation, at the processing unit, and throughout the production chain, which makes it more difficult to control [12].…”

Section: Salmonella Prevalence In Broiler Chickensmentioning

confidence: 99%

“…Identification of the biologically active substances responsible for specific biological activities in medicinal plant extracts may be challenging due to their abundance [5]. Herbal extracts work by combining various chemicals with synergistic, additive, or antagonistic effects to provide overall efficacy [12]. Further study is needed to determine the impact of synergy, increased bioavailability, cumulative effects, or additive characteristics.…”

Section: Potential Synergistic Effects Of Combining Multiple Herbal E...mentioning

confidence: 99%

Mitigation Potential of Herbal Extracts and Constituent Bioactive Compounds on Salmonella in Meat-Type Poultry

Orimaye,

Ekunseitan,

Omaliko

et al. 2024

Animals

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Herbal extracts have been widely evaluated in poultry production for their beneficial effects and potential substitute for antibiotics, which contribute to AMR and risks to human health through the consumption of infected meat. Salmonellosis is a systemic infection caused by Salmonella, an intracellular bacterium with the ability to cause systemic infections with significant implications for both the health and safety of farmers and consumers. The excessive use of antibiotics has escalated the incidence of antibiotic resistance bacteria in the poultry and livestock industry, highlighting the urgent need for alternatives especially in meat-type poultry. Both in vivo usage and in vitro studies of bioactive compounds from herbal extracts have demonstrated the effective antimicrobial activities against pathogenic bacteria, showing promise in managing Salmonella infections and enhancing poultry performance. Phytobiotic feed additives have shown promising results in improving poultry output due to their pharmacological properties, such as stimulating consumption, and enhancing antioxidant properties and preventing the increasing antimicrobial resistance threats. Despite potential for synergistic effects from plant-derived compounds, a further investigation into is essential to fully understand their role and mechanisms of action, for developing effective delivery systems, and for assessing environmental sustainability in controlling Salmonella in poultry production.

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Salmonella spp. in Chicken: Prevalence, Antimicrobial Resistance, and Detection Methods

Cited by 21 publications

References 65 publications

Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Comprehensive analysis of Salmonella in poultry meat and products in Türkiye: Prevalence, antibiotic susceptibility and genomic characterisation

Mitigation Potential of Herbal Extracts and Constituent Bioactive Compounds on Salmonella in Meat-Type Poultry

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