Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity

Kolodziejek, Anna M.; Altura, Melissa A.; Fan, Junping; Petersen, Erik; Cook, Matthew A.; Brzović, Peter S.; Miller, Samuel I.

doi:10.1016/j.celrep.2019.04.021

Cited by 35 publications

(29 citation statements)

References 41 publications

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“…phagosome via alteration of cholesterol through a glycerol cholesterol transferase effector protein and host oxysterol-binding protein that stimulates actin polymerisation and removal from the phagosome(Kolodziejek et al, 2019;LaRock, Brzovic, Levin, Blanc, & Miller, 2012;Meresse et al, 2001). The available data support a model in which endosomal tubulation is a byproduct necessary to maintenance of the SCV.…”

mentioning

confidence: 66%

“…As intracellular Salmonella require a source of host membrane to both stabilise the SCV and provide space upon the initiation of replication, the trafficking and metabolism of host lipids are also of great interest. Recent findings indicate that several effectors partner to recruit oxysterol binding protein 1 from the Golgi apparatus to the SCV (Kolodziejek et al, 2019). Essential proteins such as OSBP1 are an exciting area of investigation in mammalian cell biology in which proteins that form membrane/membrane contact points for lipid movement by conformational change are essential for a variety of endosomal trafficking events separate from SNARE proteins.…”

Section: Typhimurium Systemic Infection Alison O'brien and Colleaguementioning

confidence: 99%

“…These endosomal tubulations are microtubule motor‐based extensions of the SCV which have been termed Salmonella‐ induced filaments (Sif; Garcia‐del Portillo, Zwick, Leung, & Finlay, ). In addition to Sif formation, secreted effectors also modify the phagosome via alteration of cholesterol through a glycerol cholesterol transferase effector protein and host oxysterol‐binding protein that stimulates actin polymerisation and removal from the phagosome (Kolodziejek et al, ; LaRock, Brzovic, Levin, Blanc, & Miller, ; Meresse et al, ). The available data support a model in which endosomal tubulation is a byproduct necessary to maintenance of the SCV.…”

mentioning

confidence: 99%

“…The available data support a model in which endosomal tubulation is a byproduct necessary to maintenance of the SCV. Effector proteins recruit the microtubule network through the effector SifA binding to an adaptor protein, while other effectors-including SseJ and SseL-recruit lipid transport proteins to facilitate lipid movement to the SCV for its maintenance and expansion(Kolodziejek et al, 2019). The SPI2 type III secretion system also promotes microtubule-aided movement of the SCV, first towards the perinuclear region of the cell during early infection and later towards the perimeter of the cell(Guignot et al, 2004).…”

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confidence: 99%

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The cellular microbiology of Salmonellae interactions with macrophages

Petersen

Miller

2019

Cellular Microbiology

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Salmonellae are important enteric pathogens that cause gastroenteritis and systemic illnesses. Macrophages are important components of both the innate and acquired immune system, acting as phagocytes with significant antimicrobial killing activities that present antigen to the adaptive immune system. Macrophages can also be cultured from a variety of sites as primary cells, and the study of the survival and interactions of Salmonellae with these cells is a very early model of infection and cellular microbiology. This review traces the history of discoveries made using Salmonellae infection of macrophages and addresses the possibility of future research in this area, in particular with regards to understanding the complexity of individual bacteria and macrophage cell variability and how such heterogeneity may alter the outcome of infection.

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confidence: 66%

Section: Typhimurium Systemic Infection Alison O'brien and Colleaguementioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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The cellular microbiology of Salmonellae interactions with macrophages

Petersen

Miller

2019

Cellular Microbiology

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“…Furthermore, SseL works together with SseJ effector protein and alters the localization of lipid transporter oxysterol binding protein 1 (OSBP1) in S. enterica serovar Typhimurium. Deletion of both effectors increases the release of bacteria into the cytoplasm, which proves their important influence on vacuolar stability [142].…”

Section: Salmonella Produces Various Effectorsmentioning

confidence: 96%

The Ubiquitination System within Bacterial Host–Pathogen Interactions

et al. 2021

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Ubiquitination of proteins, like phosphorylation and acetylation, is an important regulatory aspect influencing numerous and various cell processes, such as immune response signaling and autophagy. The study of ubiquitination has become essential to learning about host–pathogen interactions, and a better understanding of the detailed mechanisms through which pathogens affect ubiquitination processes in host cell will contribute to vaccine development and effective treatment of diseases. Pathogenic bacteria (e.g., Salmonella enterica, Legionella pneumophila and Shigella flexneri) encode many effector proteins, such as deubiquitinating enzymes (DUBs), targeting the host ubiquitin machinery and thus disrupting pertinent ubiquitin-dependent anti-bacterial response. We focus here upon the host ubiquitination system as an integral unit, its interconnection with the regulation of inflammation and autophagy, and primarily while examining pathogens manipulating the host ubiquitination system. Many bacterial effector proteins have already been described as being translocated into the host cell, where they directly regulate host defense processes. Due to their importance in pathogenic bacteria progression within the host, they are regarded as virulence factors essential for bacterial evasion. However, in some cases (e.g., Francisella tularensis) the host ubiquitination system is influenced by bacterial infection, although the responsible bacterial effectors are still unknown.

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Loss to gain: pseudogenes in microorganisms, focusing on eubacteria, and their biological significance

Yang,

Wang,

Qaidi

et al. 2024

Appl Microbiol Biotechnol

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Pseudogenes are defined as “non-functional” copies of corresponding parent genes. The cognition of pseudogenes continues to be refreshed through accumulating and updating research findings. Previous studies have predominantly focused on mammals, but pseudogenes have received relatively less attention in the field of microbiology. Given the increasing recognition on the importance of pseudogenes, in this review, we focus on several aspects of microorganism pseudogenes, including their classification and characteristics, their generation and fate, their identification, their abundance and distribution, their impact on virulence, their ability to recombine with functional genes, the extent to which some pseudogenes are transcribed and translated, and the relationship between pseudogenes and viruses. By summarizing and organizing the latest research progress, this review will provide a comprehensive perspective and improved understanding on pseudogenes in microorganisms. Key points • Concept, classification and characteristics, identification and databases, content, and distribution of microbial pseudogenes are presented. • How pseudogenization contribute to pathogen virulence is highlighted. • Pseudogenes with potential functions in microorganisms are discussed.

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Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity

Cited by 35 publications

References 41 publications

The cellular microbiology of Salmonellae interactions with macrophages

The cellular microbiology of Salmonellae interactions with macrophages

The Ubiquitination System within Bacterial Host–Pathogen Interactions

Loss to gain: pseudogenes in microorganisms, focusing on eubacteria, and their biological significance

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