Salmonella Typhimurium infection primes a nutriprive mechanism in piglets

Miarelli, M.; Drumo, Rosanna; Signorelli, Federica; Marchitelli, Cinzia; Pavone, Silvia; Pesciaroli, Michele; Ruggieri, Jessica; Chirullo, Barbara; Ammendola, Serena; Battistoni, Andrea; Alborali, G. L.; Manuali, Elisabetta; Pasquali, Paolo

doi:10.1016/j.vetmic.2016.02.006

Cited by 2 publications

(4 citation statements)

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“…After IHC labeling and image acquisition, the load of Salmonella was quantified in 10 images for each preparation using the IHC profiler plugin in ImageJ. 28 The IHC profiler algorithm, which estimates the number of positive pixels in each image, was used to enumerate Salmonella and macrophages. Neutrophils were enumerated by counting each cell type in 10 randomly selected fields (400× field) from each cut processed.…”

Section: Methodsmentioning

confidence: 99%

Salmonella Typhimurium Infection Along the Porcine Gastrointestinal Tract and Associated Lymphoid Tissues

et al. 2019

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Salmonella is a major foodborne pathogen and pork is one of the main sources of human salmonellosis. Understanding the pathogenesis and progression of the infection within the host is of interest to establish potential approaches to control the disease in pigs. The present study evaluates factors such as intestinal colonization, fecal shedding, and pathogen persistence by 2 studies using experimental challenge with Salmonella Typhimurium in weaned pigs and euthanasia at different time points (1, 2, and 6 and 2, 14, and 30 days postinfection [dpi], respectively). Histopathology of intestine at early time points (1 dpi and 2 dpi) showed severe damage to the epithelium together with an increase in polymorphonuclear cells and macrophages ( P < .001), particularly in jejunum and ileum. Large quantities of Salmonella were detected within the contents of the ileum, cecum, and colon in early infection. Salmonella could also be observed in the medulla of tonsils and mesenteric lymph nodes. From 6 dpi onward, signs of recovery were observed, with progressive restoration of the epithelium, reduction of the inflammatory infiltrate, and elimination of Salmonella from the mucosa. Concentration of Salmonella in feces and ileum content decreased, but shedding did not cease even at 4 weeks after infection. Persistence of the bacteria in mesenteric lymph nodes was identified within the connective tissue at 14 and 30 dpi. Our results demonstrate a recovery of the disease after an initial acute phase but also show persistence within the lumen and surrounding lymphoid tissue. These findings are relevant to developing effective control strategies.

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Section: Methodsmentioning

confidence: 99%

Salmonella Typhimurium Infection Along the Porcine Gastrointestinal Tract and Associated Lymphoid Tissues

et al. 2019

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“…One way in which S. Typhimurium may modify carbon metabolism is via AvrA, a T3SS effector, which mediates p53 acetylation [150]. This downregulation of the glycolytic pathway was also shown in several proteome studies using porcine intestinal mucosa [151,152]. The DIGE‐based proteomic approach to the small intestinal mucosa of naturally infected pigs showed that the metabolism of carbohydrates, lipids, small molecules, vitamins and minerals was significantly altered following infection [151] with downregulation of retinal dehydrogenase 1, GAPDH, phosphoglycerate mutase1 and aldolase B, which are involved in gluconeogenesis/glycolysis.…”

Section: Alterations In Host Metabolism In Response To Gut Pathogensmentioning

confidence: 97%

“…In addition, a significant downregulation of mitochondrial ATP synthase and other enzymes involved in the TCA cycle and fatty acid oxidation, such as enoyl-CoA hydratase, aconitase 2, citrate synthase and creatine kinase, was also seen. A study on the caecal intestinal mucosa of piglets infected with virulent or attenuated S. Typhimurium strains revealed perturbation in energy metabolism and cytoskeleton-related proteins [152]. In contrast, other studies showed reduced levels of enzymes involved in oxidative phosphorylation and GAPDH [151,153].…”

Section: Salmonella Typhimuriummentioning

confidence: 98%

“…A study on the caecal intestinal mucosa of piglets infected with virulent or attenuated S . Typhimurium strains revealed perturbation in energy metabolism and cytoskeleton‐related proteins [152]. In contrast, other studies showed reduced levels of enzymes involved in oxidative phosphorylation and GAPDH [151,153].…”

Section: Alterations In Host Metabolism In Response To Gut Pathogensmentioning

confidence: 99%

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The metabolic impact of bacterial infection in the gut

2022

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Bacterial infections of the gut are one of the major causes of morbidity and mortality worldwide. The interplay between the pathogen and the host is finely balanced, with the bacteria evolving to proliferate and establish infection. In contrast, the host mounts a response to first restrict and then eliminate the infection. The intestine is a rapidly proliferating tissue, and metabolism is tuned to cater to the demands of proliferation and differentiation along the crypt–villus axis (CVA) in the gut. As bacterial pathogens encounter the intestinal epithelium, they elicit changes in the host cell, and core metabolic pathways such as the tricarboxylic acid (TCA) cycle, lipid metabolism and glycolysis are affected. This review highlights the mechanisms utilized by diverse gut bacterial pathogens to subvert host metabolism and describes host responses to the infection.

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Salmonella Typhimurium infection primes a nutriprive mechanism in piglets

Cited by 2 publications

References 36 publications

Salmonella Typhimurium Infection Along the Porcine Gastrointestinal Tract and Associated Lymphoid Tissues

Salmonella Typhimurium Infection Along the Porcine Gastrointestinal Tract and Associated Lymphoid Tissues

The metabolic impact of bacterial infection in the gut

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