Salmonella Typhimurium LPS mutations for use in vaccines allowing differentiation of infected and vaccinated pigs

Leyman, Bregje; Boyen, Filip; Parys, Alexander Van; Verbrugghe, Elin; Haesebrouck, Freddy; Pasmans, Frank

doi:10.1016/j.vaccine.2011.03.004

Cited by 44 publications

(41 citation statements)

References 22 publications

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“…Vaccination may thus hamper the use of serology for Salmonella monitoring programmes. Programmes that are based on bacteriology, new diagnostic tests that do not detect vaccine-induced antibodies (Selke et al, 2007), or new vaccines that are not detected by current ELISA methods (Leyman et al, 2011) are necessary if vaccination is to become a routine part of Salmonella control.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of three intervention strategies to reduce the transmission of Salmonella Typhimurium in pigs

Ridder

Maes

Dewulf

et al. 2013

The Veterinary Journal

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a b s t r a c tDespite current control measures, Salmonella in pigs remains a major public health concern. In this in vivo study, the effect of three intervention strategies on Salmonella Typhimurium transmission in pigs was evaluated. The first intervention was feed supplemented with coated calcium-butyrate (group A); the second comprised oral vaccination with a double-attenuated Salmonella Typhimurium strain (group B), and the third was acidification of drinking water with a mixture of organic acids (group C). After challenge at 8 weeks of age, animals were individually sampled for 6 weeks (blood once per week; faeces twice per week) and then were euthanased at 14 weeks of age. Post-mortem ileum, caecum, ileocaecal lymph nodes, and tonsils were sampled, along with ileal, caecal and rectal contents, and tested for the presence of Salmonella spp. Transmission was quantified by calculating an 'adjusted' reproduction ratio 'R a ' and its 95% confidence interval (CI).The proportion of pigs that excreted Salmonella spp. via the faeces was significantly higher in group C (58%, P < 0.0001) and the positive control group (41%, P = 0.03), compared to group B (15%), and the proportion in group C was also significantly higher than in group A (23%, P = 0.01). Group A had the lowest proportion of positive post-mortem samples (18%), followed by group B (31%), the positive control group (41%) and group C (64%) (P < 0.03). The highest transmission was seen in the positive control group and group C (R a = +1 with 95% CI [1.88; +1]), followed by group B (R a = 2.61 [1.21; 9.45]) and A (R a = 1.76 [1.02; 9.01]). The results of this study suggest that vaccination and supplementation of the feed with coated calcium-butyrate limited Salmonella transmission in pigs and might be useful control measures.

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Section: Discussionmentioning

confidence: 99%

Evaluation of three intervention strategies to reduce the transmission of Salmonella Typhimurium in pigs

Ridder

Maes

Dewulf

et al. 2013

The Veterinary Journal

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“…Vaccination is one of the methods suggested to decrease the burden of S. Typhimurium at the swine farm level and to prevent dissemination of bacterial microorganisms through the pork production chain. Vaccine efficacy could benefit from the association with other prophylaxis measures such as the administration of prebiotics, probiotics and organic acids in feed or water [11,12].…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the use of vaccines could represent a valuable solution to increase the resistance of pigs to infection and minimize the spread of Salmonella spp. in the environment [11,12]. Attenuated vaccines are known to be more effective than those based on killed-microorganisms, being able to http://dx.doi.org/10.1016/j.vaccine.2014.05.066 0264-410X/© 2014 Elsevier Ltd. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

Parenteral administration of attenuated Salmonella Typhimurium ΔznuABC is protective against salmonellosis in piglets

Ruggeri

Pesciaroli

Gaetarelli

et al. 2014

Vaccine

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a b s t r a c tA major cause of salmonellosis in humans is the contamination of pork products. Infection in pigs can be controlled using bio-security programs, but they are not sufficient in countries where a high level of infection is recorded. In this context, the use of vaccines can represent a valid supplementary method of control. Recently, we have demonstrated that an attenuated strain of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium znuABC) is protective against systemic and enteric salmonellosis in mouse and pig infection models, candidating this strain as an oral attenuated vaccine. In this study, we compared the efficacy of this attenuated Salmonella Typhimurium strain when administered orally or parenterally. Furthermore, in order to reproduce a pseudo-natural infection model, vaccinated pigs were allocated in the same pen with animals shedding virulent Salmonella Typhimurium. Animals were monitored weekly after vaccination and contact with infected piglets. Diarrhea and ataxia were recorded and Salmonella shedding was tested individually through bacterial culture. After four weeks of cohousing, piglets were euthanized, after which lymph nodes reactivity and gross lesions of the gut sections were scored at necropsy. Organs were submitted to microbiological and histological analyses.The data reported herein show that parenterally vaccinated animals do not shed the attenuated strain, and at the same time the absence of symptoms and decrease in virulent strain shedding in feces from day 6 after challenge demonstrated protection against infection induced by virulent Salmonella Typhimurium. In conclusion, our findings suggest that this is an alternative route of Salmonella Typhimurium znuABC administration, without ignoring the advantages associated with oral vaccination.

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“…In this case, vaccination is considered a major control measure to reduce Salmonella contamination at the early stages of meat production (Selke et al, 2007;Eddicks et al, 2009;Leyman et al, 2011;Xu et al, 2012;Pesciaroli et al, 2013).…”

Section: Vaccinesmentioning

confidence: 99%

Salmonellosis in swine: Clinical perspectives

Shim¹,

Hong²,

Seok³

et al. 2016

Korean Journal of Agricultural Science

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Salmonella is one of the most important food-borne zoonotic pathogens, causing acute or chronic digestive diseases such as enteritis. The acute form of enteritis is common in young pigs of 2 -4 months of age. The main symptoms include high fever (41 -42℃), loss of appetite, and increased mortality within 2 -4 days of onset of the disease. It is often the cause of increasing mortality, decreasing growth rate and reducing feed efficiency of piglets. In the case of chronic enteritis in pigs, the main symptom is weight loss due to the continuing severe diarrhea. Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Choleraesuis are typical pig adapted serotypes, which cause one of four major syndromes: enteric fever, enterocolitis/diarrhea, bacteremia and chronic asymptomatic carriage. These syndromes cause a huge economic burden to swine industry by reducing production. Therefore, it is necessary that swine industries should strive to decrease Salmonellosis in pigs in order to reduce economic losses. There are several measures, such as vaccination to prevent salmonellosis, that are implemented differently from country to country. For the treatment of Salmonella, ongoing antibiotic treatment is needed. However constant doses of antibiotics can be a problem because of antibiotic resistance. Therefore, the focus should be made more on prevention than treatment. In this review, we addressed the basic information about Salmonella, route of infection, clinical symptoms, and prevention of Salmonellosis.

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Salmonella Typhimurium LPS mutations for use in vaccines allowing differentiation of infected and vaccinated pigs

Abstract: 26

Cited by 44 publications

References 22 publications

Evaluation of three intervention strategies to reduce the transmission of Salmonella Typhimurium in pigs

Evaluation of three intervention strategies to reduce the transmission of Salmonella Typhimurium in pigs

Parenteral administration of attenuated Salmonella Typhimurium ΔznuABC is protective against salmonellosis in piglets

Salmonellosis in swine: Clinical perspectives

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