Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of<i>N</i>-Glycans: Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation

Kimura, Yoshinobu; Watanabe, Takao; Kimura, M.; Maeda, Megumi; Murata, Yoshiyuki; Fujiyama, Kazuhito

doi:10.1271/bbb.70592

Cited by 2 publications

(1 citation statement)

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“…Different studies in the literature have focused on the accumulation of AGPs in culture medium during salt-adaptation in cell cultures (Zhu et al, 1993; Lamport et al, 2006; Kimura et al, 2008; Zargochev and Odjakova, 2011). However, some important discrepancies are produced between them and our study regarding the accumulation of AGPs in the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Arabinogalactan Proteins Are Involved in Salt-Adaptation and Vesicle Trafficking in Tobacco by-2 Cell Cultures

et al. 2017

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Arabinogalactan proteins (AGPs) are a highly diverse family of glycoproteins that are commonly found in most plant species. However, little is known about the physiological and molecular mechanisms of their function. AGPs are involved in different biological processes such as cell differentiation, cell expansion, tissue development and somatic embryogenesis. AGPs are also involved in abiotic stress response such as salinity modulating cell wall expansion. In this study, we describe how salt-adaptation in tobacco BY-2 cell cultures induces important changes in arabinogalactan proteins distribution and contents. Using the immuno-dot blot technique with different anti-AGP antibodies (JIM13, JIM15, and others), we observed that AGPs were highly accumulated in the culture medium of salt-adapted tobacco cells, probably due to the action of phospholipases. We located these AGP epitopes using immunogold labeling in the cytoplasm associated to the endoplasmic reticulum, the golgi apparatus, and vesicles, plasma membrane and tonoplast. Our results show that salt-adaptation induced a significant reduction of the cytoplasm, plasma membrane and tonoplast content of these epitopes. Yariv reagent was added to the control and salt-adapted tobacco cell cultures, leading to cell death induction in control cells but not in salt-adapted cells. Ultrastructural and immunogold labeling revealed that cell death induced by Yariv reagent in control cells was due to the interaction of Yariv reagent with the AGPs linked to the plasma membranes. Finally, we propose a new function of AGPs as a possible sodium carrier through the mechanism of vesicle trafficking from the apoplast to the vacuoles in salt-adapted tobacco BY-2 cells. This mechanism may contribute to sodium homeostasis during salt-adaptation to high saline concentrations.

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