Salt-dependent elution of uncharged aromatic solutes in ion-exchange chromatography

Hirano, Atsushi; Iwashita, Kazuki; Sakuraba, Shun; Shiraki, Kentaro; Arakawa, Tsutomu; Kameda, Tomoshi

doi:10.1016/j.chroma.2018.02.049

Cited by 12 publications

(7 citation statements)

References 30 publications

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“…More interestingly, the IENFAs presented an specific selectivity toward positively charged template proteins ascribing to the electronegativity of carboxyl ligands, which was confirmed by the result of the coomassie brilliant blue (CBB) G250 staining method (Figure S36, Supporting Information) . Furthermore, the protein adsorption capacity of the IENFAs could be facilely regulated by adjusting the ionic strength and pH value of the PBS (Figure S37, Supporting Information), exhibiting their convenient elution property and outstanding controllability . In terms of the evaluation of the cycle performance, to fully remove all of the adsorbed and loosely bound proteins on the IENFAs, those samples were completely washed by using PBS containing 1 m NaCl and 0.1 wt% Tween 20 after each adsorption cycle.…”

Section: Resultsmentioning

confidence: 99%

Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation

Duan

et al. 2019

Adv Funct Materials

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Chromatographic media with synchronously large protein adsorption capacity and high processing flux are highly desired in protein separation; however, the creation of such materials still faces enormous challenges. Herein, a robust strategy to develop highly carboxylated monolithic media by combining nanofibrous aerogels' forming technique and an in situ modification approach is reported. The obtained ion-exchange nanofibrous aerogels (IENFAs) exhibit a unique cellular structure consisting of flexible ceramic nanofibers and a functional polymer wrapping layer, endowing them with outstanding underwater superelasticity and compressive fatigue resistance (nearly no plastic deformation after 1000 compressive cycles). Benefiting from the interconnected nanofibrous cellular structure, good hydrophilicity, high carboxylation, and excellent mechanical properties, the IENFAs exhibit synchronously promoted static (2.9 × 10 3 mg g −1 ) and dynamic (1.7 × 10 3 mg g −1 ) lysozyme adsorption capacities and improved buffer flux (2.17 × 10 4 L m −2 h −1 , gravity driven), which are superior to these reported nanofibrous materials and commercial ion-exchange membranes. The IENFAs also possess outstanding performance stability, easy operation, and excellent regenerability. Moreover, the IENFA-packed column could directly and continuously separate lysozyme from egg white solely driven by gravity, highlighting their excellent practical application performance. This work may provide a new avenue to design and develop next-generation high-performance chromatographic media for bioseparation.

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Section: Resultsmentioning

confidence: 99%

Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation

Duan

et al. 2019

Adv Funct Materials

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“…Umbrella sampling simulations were performed to determine the binding free energy profiles of these systems. In this study, we used the umbrella integration (UI) method , similar to that used in our previous studies . In the umbrella sampling simulations, changes in the binding free energy A (ξ) along the order parameter (ξ) were generally obtained by combining the potential mean force (PMF) along ξ using MD simulations.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we used the umbrella integration (UI) method 37,38 similar to that used in our previous studies. 39 In the umbrella sampling simulations, changes in the binding free energy A(ξ) along the order parameter (ξ) were generally obtained by combining the potential mean force (PMF) along ξ using MD simulations. To sample the order parameter effectively, the binding pathway is separated into two windows at the center, and then, the system is fixed in a specific window by using a series of bias potentials.…”

Section: ■ Methodsmentioning

confidence: 99%

Aromaphilicity Index of Amino Acids: Molecular Dynamics Simulations of the Protein Binding Affinity for Carbon Nanomaterials

Hirano

Kameda

2021

ACS Appl. Nano Mater.

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Aromatic carbon nanomaterials, such as carbon nanotubes and graphene, undergo protein adsorption in the early stages of their uptake into biological systems, which determines their bioavailability and cytotoxicity. Although a mechanistic understanding of protein–nanomaterial interactions is essential for realizing safe and controlled in vivo applications of nanomaterials, it remains challenging to predict arbitrary protein–nanomaterial interactions. This study introduces an index, “aromaphilicity (aromatic-loving nature) index”, for 20 proteinogenic amino acids. This index reflects the affinity of the amino acid side chains for the aromatic carbon surfaces, which was quantified by molecular dynamics simulations. This index is significantly correlated with the experimental data (R 2 = 0.789) and successfully utilized as a versatile tool for predicting the affinity hot spots of the proteins for the aromatic carbon nanomaterials. This approach advances the understanding of the mechanism of protein–nanomaterial interactions and improves prediction of the biological impacts of nanomaterials.

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“…Umbrella sampling simulations were performed to determine the binding free energy profiles of these systems. In this study, we used the umbrella integration (UI) method , similar to that used in our previous studies. , In the umbrella sampling simulations, changes in the binding free energy A (ξ) along the order parameter ξ were generally acquired by combining the potential mean force (PMF) along ξ using MD simulations. To sample the ξ effectively, the binding pathway was separated into two windows at the center, and the system was then fixed in a specific window using a series of bias potentials.…”

Section: Methodsmentioning

confidence: 99%

Diameter-Selective Sorting of Single-Walled Carbon Nanotubes Using π-Molecular Tweezers for Energy Materials

El-Refaey

Kozawa

Kameda

et al. 2023

ACS Appl. Nano Mater.

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Single-walled carbon nanotubes (SWCNTs) have remarkable electrical properties, making them excellent candidate energy materials for applications, such as transistors and thermoelectric devices. However, as-prepared SWCNTs are mixtures of various chiral indices associated with different diameters, and this heterogeneity hinders their practical applications. Therefore, the development of a method for sorting SWCNTs according to the diameter is required. Here, we report one-pot diameter sorting of SWCNTs through noncovalent functionalization with π-molecular tweezers, which act as a structure-selective oligomeric dispersant for SWCNTs. The tweezers are capable of isolating (9,4) SWCNTs and (7,6) SWCNTs with diameters of ∼0.9 nm from as-prepared SWCNTs to a sorting ratio of 90%. This high-diameter selectivity originates from molecular wrapping of the π-molecular tweezers around the SWCNT sidewalls based on π–π and hydrophobic interactions. Our findings are promising not only in terms of developing a suitable SWCNT sorting methodology but also for understanding their molecular-level sorting mechanism.

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Salt-dependent elution of uncharged aromatic solutes in ion-exchange chromatography

Cited by 12 publications

References 30 publications

Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation

Highly Carboxylated, Cellular Structured, and Underwater Superelastic Nanofibrous Aerogels for Efficient Protein Separation

Aromaphilicity Index of Amino Acids: Molecular Dynamics Simulations of the Protein Binding Affinity for Carbon Nanomaterials

Diameter-Selective Sorting of Single-Walled Carbon Nanotubes Using π-Molecular Tweezers for Energy Materials

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