Salt-Induced Changes in Cytosolic pH and Photosynthesis in Tobacco and Potato Leaves

Pecherina, Anna; Grinberg, Marina; Ageyeva, Maria; Zanegina, Daria; Akinchits, Elena; Brilkina, Anna A.; Vodeneev, Vladimir

doi:10.3390/ijms24010491

Cited by 15 publications

(13 citation statements)

References 103 publications

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“…Previous reports addressing the changes in pHcyt upon saline (NaCl) treatment have yielded inconsistent results. Salt-induced cytoplasmic alkalinization [ 63 , 64 ], acidification [ 65 ], no significant response [ 66 , 67 ], and even the opposite dynamics of pHcyt depending on the contrasting salt tolerance of rice cultivars [ 68 ] or treatment length [ 69 ] have been described. However, these reports were carried out with internalized fluorescent chemical probes, protoplasts, in single-cell systems or leaves, or were restricted to predefined root segments.…”

Section: Discussionmentioning

confidence: 99%

Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway

Rombolá-Caldentey

Andrés

Waadt

et al. 2023

IJMS

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Plants have evolved elaborate mechanisms to sense, respond to and overcome the detrimental effects of high soil salinity. The role of calcium transients in salinity stress signaling is well established, but the physiological significance of concurrent salinity-induced changes in cytosolic pH remains largely undefined. Here, we analyzed the response of Arabidopsis roots expressing the genetically encoded ratiometric pH-sensor pHGFP fused to marker proteins for the recruitment of the sensor to the cytosolic side of the tonoplast (pHGFP-VTI11) and the plasma membrane (pHGFP-LTI6b). Salinity elicited a rapid alkalinization of cytosolic pH (pHcyt) in the meristematic and elongation zone of wild-type roots. The pH-shift near the plasma membrane preceded that at the tonoplast. In pH-maps transversal to the root axis, the epidermis and cortex had cells with a more alkaline pHcyt relative to cells in the stele in control conditions. Conversely, seedlings treated with 100 mM NaCl exhibited an increased pHcyt in cells of the vasculature relative to the external layers of the root, and this response occurred in both reporter lines. These pHcyt changes were substantially reduced in mutant roots lacking a functional SOS3/CBL4 protein, suggesting that the operation of the SOS pathway mediated the dynamics of pHcyt in response to salinity.

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Section: Discussionmentioning

confidence: 99%

Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway

Rombolá-Caldentey

Andrés

Waadt

et al. 2023

IJMS

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“…Changes in pH are also observed in the case of salinity and have been recorded in the root and shoot cells of various plant species, including Arabidopsis [25][26][27][28], tobacco and potato [29], quince protoplasts [30], beans protoplasts [31], quinoa and peas protoplasts [32] and rice protoplasts [33]. Salinity-induced pHcyt changes can be of either direction.…”

Section: Introductionmentioning

confidence: 94%

“…Salinity-induced pHcyt changes can be of either direction. Some studies report cytosolic acidification [25][26][27]29,32], while others indicate cytosolic alkalinization [28,[30][31][32]. Investigations focusing on salinity-induced tissue-specific pH changes in intact roots across a wide range of NaCl concentrations have rarely been published and they have mainly been conducted on Arabidopsis [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…The presence of fluorescent proteins in the cytosol also enables the visualization of cell morphology and different tissue types without the need for histological staining. Pt-GFP, a widely used genetically encoded ratiometric pH sensor [23,26,27,29,[35][36][37], boasts a broad pH sensitivity range (pH 4.5-8.5), exceptional acid stability, and insensitivity to salinity [26]. In this study, we employed transgenic tobacco plants expressing a pH-sensitive sensor Pt-GFP to investigate how salinity affects pHcyt in root cells.…”

Section: Introductionmentioning

confidence: 99%

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The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (Nicotiana tabacum L.): Nonlinear Effects of NaCl Concentrations

Ageyeva,

Zdobnova,

Nazarova

et al. 2023

Plants

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Salinity impacts important processes in plants, reducing their yield. The effect of salinity on the cytosolic pH (pHcyt) has been little studied. In this research, we employed transgenic tobacco plants expressing the pH sensor Pt-GFP to investigate the alterations in pHcyt in cells across various root zones. Furthermore, we examined a wide spectrum of NaCl concentrations (ranging from 0 to 150 mM) and assessed morphological parameters and plant development. Our findings revealed a pattern of cytosolic acidification in cells across all root zones at lower NaCl concentrations (50, 100 mM). Interestingly, at 150 mM NaCl, pHcyt levels either increased or returned to normal, indicating a nonlinear effect of salinity on pHcyt. Most studied parameters related to development and morphology exhibited an inhibitory influence in response to NaCl. Notably, a nonlinear relationship was observed in the cell length within the elongation and differentiation zones. While cell elongation occurred at 50 and 100 mM NaCl, it was not evident at 150 mM NaCl. This suggests a complex interplay between stimulating and inhibitory effects of salinity, contributing to the nonlinear relationship observed between pHcyt, cell length, and NaCl concentration.

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“…In this case, the pH cyt decrease was inhibited by NH 4 VO 3 , an inhibitor of H + ATPase at the plasma membrane, and could be caused by activation of the Na + /H + antiporter SOS1 in the plasma membrane, which is energized by the PM H + ATPase [ 300 , 301 ]. Experiments with leaves from salt-sensitive tobacco and potato plants that were transformed by a gene for a pH-sensitive protein, Pt-GFP, also reacted in the same way: salt treatment caused a decrease in pH cyt , and depended on the time the plants were subjected to the stressor [ 302 ].…”

Section: Ph Changes and Signaling Under Salinitymentioning

confidence: 99%

Ion Changes and Signaling under Salt Stress in Wheat and Other Important Crops

Lindberg,

Premkumar

2023

Plants

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High concentrations of sodium (Na+), chloride (Cl−), calcium (Ca2+), and sulphate (SO42−) are frequently found in saline soils. Crop plants cannot successfully develop and produce because salt stress impairs the uptake of Ca2+, potassium (K+), and water into plant cells. Different intracellular and extracellular ionic concentrations change with salinity, including those of Ca2+, K+, and protons. These cations serve as stress signaling molecules in addition to being essential for ionic homeostasis and nutrition. Maintaining an appropriate K+:Na+ ratio is one crucial plant mechanism for salt tolerance, which is a complicated trait. Another important mechanism is the ability for fast extrusion of Na+ from the cytosol. Ca2+ is established as a ubiquitous secondary messenger, which transmits various stress signals into metabolic alterations that cause adaptive responses. When plants are under stress, the cytosolic-free Ca2+ concentration can rise to 10 times or more from its resting level of 50–100 nanomolar. Reactive oxygen species (ROS) are linked to the Ca2+ alterations and are produced by stress. Depending on the type, frequency, and intensity of the stress, the cytosolic Ca2+ signals oscillate, are transient, or persist for a longer period and exhibit specific “signatures”. Both the influx and efflux of Ca2+ affect the length and amplitude of the signal. According to several reports, under stress Ca2+ alterations can occur not only in the cytoplasm of the cell but also in the cell walls, nucleus, and other cell organelles and the Ca2+ waves propagate through the whole plant. Here, we will focus on how wheat and other important crops absorb Na+, K+, and Cl− when plants are under salt stress, as well as how Ca2+, K+, and pH cause intracellular signaling and homeostasis. Similar mechanisms in the model plant Arabidopsis will also be considered. Knowledge of these processes is important for understanding how plants react to salinity stress and for the development of tolerant crops.

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Salt-Induced Changes in Cytosolic pH and Photosynthesis in Tobacco and Potato Leaves

Cited by 15 publications

References 103 publications

Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway

Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway

The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (Nicotiana tabacum L.): Nonlinear Effects of NaCl Concentrations

Ion Changes and Signaling under Salt Stress in Wheat and Other Important Crops

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