Salt-induced changes in the antioxidant system and viability of oilseed rape

Abstract

This work was aimed to investigate the effect of salt (NaCl) stress on three oilseed rape (Brassica napus L.) cultivars: 'Banacanka', 'Jasna' and 'Kata'. The following parameters were determined: antioxidant enzymes superoxide dismutase (SOD) and guaiacol peroxidase (GPX), reduced glutathione (GSH) quantity, lipid peroxidation (LP) intensity, radical-scavenging capacity (DPPH-RSC), hydroxyl radical scavenging (• OH radicals) and ferric reducing antioxidant power (FRAP). Seed viability was determined using the standard laboratory test for germination as well as the seed accelerated aging test measuring the germination, shoot and root length. The plants were subjected to three salt treatments: 0, 100, 150 and 200 mmol dm -3 NaCl for 7 days. All three cultivars responded with an increase of lipid peroxidation in seedlings and with a decrease of GPX activity. 'Banacanka' was found to be the most vulnerable cultivar, with an increase of 237% of LP intensity in comparison to the control at the highest NaCl concentration. The induction of the SOD activity was observed for all three cultivars by different NaCl concentrations. The antioxidant capacity measured by FRAP, DPPH-RSC and• OH radicals was also induced, especially FRAP at the highest concentration in the cultivar 'Jasna' (by 282%). Germination, shoot and root length decreased for all investigated cultivars. Our results showed that cultivar 'Jasna' is the most adapted to salt stress because of the highest SOD activity and accumulation of antioxidants. Furthermore, this research suggests that some of the antioxidant parameters can be useful biochemical markers for the selection of oilseed rape tolerant cultivars under the conditions of salinity.

The Combination of Increased Temperatures and High Irradiation Causes Changes in Photosynthetic Efficiency

“…GPOX enzyme activity increased in all the hybrids in the combined conditions of increased temperatures and high irradiation in 2014 ( Table 2 ). The same was reported in numerous other studies [ 16 , 33 , 34 , 35 ]. Results in 2013 had a different trend, as GPOX activity decreased in the afternoon conditions ( Table 2 ).…”

Drought-induced changes in antioxidant system and osmolyte content of poplar cuttings

“…The GPX is an important enzyme in the antioxidant system that works with GSH and GR to prevent the formation of hydrogen peroxide. Increased antioxidant enzyme activities in leave samples could be used as a strategy to tolerate drought (Guerrier et al 2000, Ahmed et al 2013, Jovičić et al 2017. In the control leaves samples GPX activity were observed: 0.011 (M-1), 0.018 (B-229) and 0.016 (PE19/66) U mg -1 proteins.…”

Contribution to the Optimization of Methods for Vigor Seed Evaluation of Camelina sativa (L.) Crantz

“…Simultaneously, there is an escalation of metabolic processes coupled with the ineffectiveness of enzymes responsible for neutralizing the impact of free radicals [23]. This phenomenon correlates with an elevation in lipid peroxidation levels, serving as an indicative marker of stressful environmental conditions [24,25]. Additionally, those factors can cause the carbonylation of proteins, which significantly escalates during the progression of seed decay and can eventually culminate in the death of the seed [26].…”