Salt relations of Dunaliella. Transitional changes in glycerol content and oxygen exchange reactions on water stress

Kessly, David Stuart; Brown, A. D.

doi:10.1007/bf00455353

Cited by 22 publications

(9 citation statements)

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“…cerevisiae was less able to retain glycerol, so that a far higher extracellular concentration of glycerol was present in the culture medium of this species than that of S. rouxii. Glycerol accumulated by D. parva and D. tertiolecta for osmotic adjustment did not leak following hypoosmotic shock (Ben-Amotz, 1975;Kessly & Brown, 1981). Although external concentrations of glycerol were not measured in the present experiments, the time course involved suggested that conversion to nonosmotic compounds rather than leakage took place following hypoosmotic shock.…”

Section: Discussionmentioning

confidence: 54%

Effect of Osmotic Shock on Some Intracellular Solutes in Two Filamentous Fungi

Luard

1982

Microbiology

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Hypoosmotic and hyperosmotic shock experiments confirmed the importance for osmoregulation in Penicillium chrysogenum and Chrysosporium fastidium of glycerol and the osmoticum used in the medium. Growth ceased following both types of shock treatment for a time, depending on the magnitude of the shock and the species. Regrowth following hypoosmotic shock took place some distance behind burst tips. Following hyperosmotic shock, growth was initiated by branching at the apex, but if the magnitude of the shock was greater than 10 MPa, it did not occur in either species although osmotic adjustment was observed. Shock experiments did not implicate the higher polyols in osmotic adjustment. Hypoosmotic shock to C. fastidium resulted in a rapid loss of all internal solutes, while decrease in glycerol in P . chrysogenum following this treatment was more gradual. Transfer of C. fastidium, a species which does not grow on media of high salt concentration, to isoosmotic KCl did not result in loss of turgor although growth was inhibited. Glucose was lost from the hyphae but K+ and C1-were taken up.

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Section: Discussionmentioning

confidence: 54%

Effect of Osmotic Shock on Some Intracellular Solutes in Two Filamentous Fungi

Luard

1982

Microbiology

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“…The length of the lag phase increases with the magnitude of the salinity up-shock. Brown and Borowitzka (1979) and Kessly and Brown (1981) showed that, in D. viridis, an increase in salinity from 1.5 M (=8.8% NaCI) to 4.0M (=23.3% NaCI) resulted in net 02 uptake for about 24 h. They also showed that full recovery of photosynthetic 02 evolution was inhibited by the protein synthesis inhibitors cycloheximide and chloramphenicol. On the other hand, the time taken for the glycerol content to adjust to the new osmotic regime was only about 1 h, and Sadka etal.…”

Section: Discussionmentioning

confidence: 97%

“…The effects of transferring cells of several different Dunaliella species from low to higher salinity have been documented in several papers which have examined cell volume changes, glycerol formation and photosynthesis (Brtggemann etal., 1978;Brown & Borowitzka, 1979;Kessly & Brown, 1981;Gimmler etal., 1981;Gilmour et al, 1982Gilmour et al, , 1984Sadka et al, 1989). This paper is, however, the first to examine the dynamics of carotenoid formation following a salinity upshock.…”

Section: Discussionmentioning

confidence: 99%

Effects of salinity increase on carotenoid accumulation in the green alga Dunaliella salina

1990

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The effect of sudden salinity increases on the kinetics of growth and carotenogenesis was studied in three geographically diverse isolates of Dunaliella salina. A sudden increase in salinity results in a lag phase in growth and the length of this lag phase is dependent on the final salinity and the magnitude of the salinity change (no lag at 10-15% w/v NaCI, 4-day lag at 30% NaCl). There is also a lag before an increase in the total carotenoid content can be measured following the salinity up-shock, and the length of the lag depends largely on the initial salinity and the magnitude of the salinity up-shock, whereas the rate of carotenogenesis and the final carotenoid content reached depend on the final salinity. The increase in total carotenoid content is mainly due to fl-carotene. Following the salinity up-shock (especially from 10% to 20% NaCl) the proportion of lutein as a percentage of total carotenoids decreases, whereas zeaxanthin increases. This suggests that the pathway synthesising lutein is more sensitive to salt or osmotic stress and is inhibited at higher salinities, thus leading to fl-carotene formation. The proportion of a-carotene does not change.

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“…Dunaliella salina was observed to increase in volume by 76% within 2 to 4 minutes when rapidly diluted from 1.71 M to 0.86 M NaCl . A new level of intracellular glycerol may be achieved within 30 minutes to a few hours, both in the light and in the dark, using the enzymatic mechanisms described above ( (Kessly and Brown, 1981). In Dunaliella tertiolecta (a species adapted to relatively low salt concentrations) dilution stress generally does not cause leakage of glycerol out of the cell; and leakage and possible burst of the cell membrane are only observed following a very drastic sudden dilution (Goyal, 1989).…”

Section: Compatible Solutes In the Domain Eucaryamentioning

confidence: 99%