Salt-responsive transcriptome analysis of triticale reveals candidate genes involved in the key metabolic pathway in response to salt stress

Abstract

Triticale is tolerant of many environmental stresses, especially highly resistant to salt stress. However, the molecular regulatory mechanism of triticale seedlings under salt stress conditions is still unclear so far. In this study, a salt-responsive transcriptome analysis was conducted to identify candidate genes or transcription factors related to salt tolerance in triticale. The root of salt-tolerant triticale cultivars TW004 with salt-treated and non-salt stress at different time points were sampled and subjected to de novo transcriptome sequencing. Total 877,858 uniquely assembled transcripts were identified and most contigs were annotated in public databases including nr, GO, KEGG, eggNOG, Swiss-Prot and Pfam. 59,280, 49,345, and 85,922 differentially expressed uniquely assembled transcripts between salt treated and control triticale root samples at three different time points (C12_vs_T12, C24_vs_T24, and C48_vs_T48) were identified, respectively. Expression profile and functional enrichment analysis of DEGs found that some DEGs were significantly enriched in metabolic pathways related to salt tolerance, such as reduction–oxidation pathways, starch and sucrose metabolism. In addition, several transcription factor families that may be associated with salt tolerance were also identified, including AP2/ERF, NAC, bHLH, WRKY and MYB. Furthermore, 14 DEGs were selected to validate the transcriptome profiles via quantitative RT-PCR. In conclusion, these results provide a foundation for further researches on the regulatory mechanism of triticale seedlings adaptation to salt stress in the future.

Comparative Physiological and Transcriptome Analysis Reveal the Molecular Mechanism of Melatonin in Regulating Salt Tolerance in Alfalfa (Medicago sativa L.)

“…Plants can trigger multiple biological processes to regulate gene transcription and physiological adaptation under unfavorable conditions (Deng et al, 2020 ). We examined the profiles of the genes involved in the plant-pathogen interaction pathway and starch and sucrose metabolism ( Figure 6 ).…”

Salt‑responsive transcriptome analysis of canola roots reveals candidate genes involved in the key metabolic pathway in response to salt stress

“…Transcription factors (TFs) have previously been shown to play integral roles in regulating ion, osmotic and ROS homeostasis in plants in response to salt stress 12 , 29 – 31 . Previous studies have shown that WRKY responds to salt stress through ABA signaling and regulates ROS production in plant cells 10 , in addition, Wang et al 32 reported that the bZIP gene (ThbZIP1) improves salt resistance in tobacco by preventing the accumulation of cellular ROS.…”

“…To uncover the molecular mechanisms of salt tolerance in plants, the screening of salt tolerance genes in salt tolerant species has been intensively investigated. In recent years, studies have shown that the transcription and expression of salt-responsive genes play important roles in transcriptional regulation of signal transduction, the activity of antioxidant enzyme systems, and the accumulation of osmoregulatory substances in plants 10 – 12 . Therefore the screening of salt-tolerant genes is important for a comprehensive understanding of the molecular mechanisms underlying salt tolerance in plants.…”

Transcriptomic Profile Analysis of Populus talassica × Populus euphratica Response and Tolerance under Salt Stress Conditions

“…After transcriptome analysis of P. talassica × P. euphratica under salt stress conditions were performed, the differentially expressed genes under these conditions were excavated, and pathways related to the salt stress responses of P. talassica × P. euphratica were identified. Understanding the salt tolerance mechanisms of P. talassica × P. euphratica at the molecular level will not only provide a valuable resource for future research and breeding [ 35 ] but will also help to promote the planting of P. talassica × P. euphratica in saline alkali land and provide a reference for the improvement and sustainable development and utilization of soil saline alkali land in Xinjiang in the future.…”