2011
DOI: 10.1074/jbc.m111.243824
|View full text |Cite
|
Sign up to set email alerts
|

Salt Sensitivity of Minimal Twin Arginine Translocases

Abstract: Background: Minimal twin arginine preprotein translocases consist of TatA and TatC subunits. Results: Here we show that various mutated and wild-type minimal Tat translocases are salt-sensitive. Conclusion: Salt-sensitive electrostatic interactions seem to have critical roles in the preprotein translocation activity of minimal Tat translocases. Significance: Our results represent first experimental evidence for an important role of salt-sensitive electrostatic interactions in Tat translocation.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
26
0

Year Published

2012
2012
2019
2019

Publication Types

Select...
7
2

Relationship

4
5

Authors

Journals

citations
Cited by 25 publications
(30 citation statements)
references
References 62 publications
(71 reference statements)
4
26
0
Order By: Relevance
“…Mobilities of molecular mass markers (in kDa) are shown on the left. ment [21]. The data show that both the periplasmic and medium samples contained very little of the GroEL, which was almost exclusively found in the spheroplasts.…”
mentioning
confidence: 67%
“…Mobilities of molecular mass markers (in kDa) are shown on the left. ment [21]. The data show that both the periplasmic and medium samples contained very little of the GroEL, which was almost exclusively found in the spheroplasts.…”
mentioning
confidence: 67%
“…The tatC gene is nonessential, as it encodes a component of a system that transports folded proteins from the cell to the outer membrane. Deletions of the gene compromise the integrity of the outer membrane and render it sensitive to many stresses (71)(72)(73)(74). The waaC gene (formerly rfaC) encodes heptosyltransferase I, which catalyzes a step in the synthesis of outer membrane lipopolysaccharide.…”
Section: Tradis Was Performed To Identify Genes Involved In Ir Survivalmentioning
confidence: 99%
“…4), suggesting their involvement in reduction of ferric to ferrous ion. As observed in other Gram-positive bacteria (Jongbloed et al, 2002(Jongbloed et al, , 2004Biswas et al, 2009;van der Ploeg et al, 2011), TatC in L. monocytogenes is supposed to be a substrate-docking protein recognizing the twinarginine motif of FepB that forms a TatC-FepB complex, which is translocated across the membrane through TatA. (a) (b) Fig.…”
Section: Tat-signal Peptide In Fepbmentioning
confidence: 99%
“…aureus was shown to be involved in iron-uptake (Biswas et al, 2009). Furthermore, a ywbN (a fepB homologue) mutant of B. subtilis was unable to grow in the presence of ferric ions but grew in the presence of ferrous, suggesting its possible role as a ferric reductase (van der Ploeg et al, 2011). The homologous EfeB protein in Escherichia coli O157 has been shown to reduce ferric iron (Liu et al, 2011) and transport ferrous iron by the EfeUOB tripartite complex (Cao et al, 2007).…”
Section: Introductionmentioning
confidence: 99%