Salt-stress Induced Alterations in Protein Profile and Protease Activity in the Mangrove Bruguiera parviflora

Abstract

Two-month-old seedlings of Bruguiera parvifora were treated with varying levels of NaCl (100, 200 and 400 mm) under hydroponic culture. Total proteins were extracted from leaves of control and NaCl treated plants after 7, 14, 30 and 45 d of treatment and analysed by SDS-PAGE. As visualized from SDS-PAGE, the intensity of several protein bands of molecular weight 17, 23, 32, 33 and 34 kDa decreased as a result of NaCl treatment. The degree of decrease of these protein bands seemed to be roughly proportional to the external NaCl concentration. The most obvious change concerned a 23 kDa-polypeptide (SSP-23), which disappeared after 45 d treatment in 400 mm NaCl. Moreover, the SSP-23 protein, which disappeared in B. parviflora under salinity stress, reappeared when these salinized seedlings were desalinized. These observations suggest the possible involvement of these polypeptides for osmotic adjustment under salt stress. NaCl stress also caused an increase in the activity of both acid and alkaline protease. The increasing activity of proteases functions as a signal of salt stress in B. parviflora, which induces the reduction of protein level.

Monitoring of Salt Tolerance Responses of Different Maize Inbred Lines Using Protein Profile and Protein Spectrofluorescence

“…This could be due to the inhibitory effects of salt stress on transcriptional process (Jain et al, 1993). The fast breakdown of cellular protein during salt stress might be also due to the increasing activity of acid and alkaline protease (Parida et al, 2004).…”

24-Epibrassinolide Restores the Synthesis of Proteins and Amino Acids in Brassica juncea L. Leaves Under Imidacloprid Stress

“…This results in the generation of free amino acid pool that is involved in maintaining the pH and osmotic pressure of the cell, nitrogen storage and ultimately protection of the components of cells under abiotic stress (Parida et al 2004). The decrease in amino acid content by IMI might be due to the blockage of amino acid synthesis pathways; this finding was supported by the studies carried out by Steinrücken and Amrhein (1980), who found that glyphosate pesticide causes blockage of shikimate pathway for aromatic amino acid synthesis due to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibition.…”

Impact of Aluminum Toxicity on Physiological Aspects of Barley (Hordeum vulgare L.) Cultivars and its Alleviation through Ascorbic Acid and Salicylic Acid Seed Priming

“…Hydrolysis of proteins by proteases releases amino acids which can use for storage and/or transport and for osmotic adjustment during stress in seedlings (Palma et al, 2002). The amino acid pool enlargement in the stressed plants can be attributed to a decreased protein synthesis and enhanced proteolysis (Parida et al, 2004). Proteolysis is also allied to oxidative stress results by ROS (O 2 -, H 2 O 2 , and OH -) whereas oxidative stress can modified the protein which is characterized for the production of carbonyl groups in the molecules (Azmat et al, 2007;Umebese and Motajo, 2008).…”