Salt Stress Induced Differential Proteome and Metabolome Response in the Shoots of Aeluropus lagopoides (Poaceae), a Halophyte C₄ Plant

Abstract

A proteomic approach was used to identify proteins affected by salt in the halophyte C(4) plant Aeluropus lagopoides (Poaceae) in an attempt to understand the mechanism of salt tolerance. Plants were treated with 450 mM NaCl for 10 days, and proteins were then extracted from the shoots and separated by two-dimensional polyacrylamide gel electrophoresis. A total of 1805 protein spots were detected, of which 39 were up-regulated and 44 were down-regulated by treatment with NaCl. Metabolism-related proteins were up-regulated, whereas photosynthesis-related proteins were down-regulated. Dose-dependence studies showed that the up-regulation continued at NaCl concentrations above 450 mM for defense-related proteins alone. Western blot analysis confirmed the down-regulation of RuBisCO LSU and RuBisCO SSU and severe down-regulation of RuBisCO activase. The activity of glyoxalase I increased with increasing NaCl concentration. Metabolome studies indicated up-regulation of amino acids and down-regulation of tricarboxylic acid cycle-related metabolites. These studies suggest that up-regulation of energy formation, amino acid biosynthesis, C(4) photosynthesis, and detoxification are the main strategies for salt tolerance in A. lagopoides.

Canola Seedling Response to NaCl Stress – a Proteomic Approach

“…Production of this enzyme along with carbohydrate metabolisms of Kelvin cycle and glycolysis increases under salinity condition. As has been reported by researches in rice where Fructose-bisphosphate aldolase peaked with increase in glycolytic enzymes and enzymes involved in anaerobic glycolytic and glycolate metabolism (Abbasi and Komatsu, 2004), as well as a peak in Alcohol dehydrogenases in canola (Sobhanian et al, 2010) under salinity influence. It has also been hypothesized that Fructose-bisphosphate aldolase can contribute to ion division in vacuoles, since it is directly interact with H + -ATP and active transportation (Barkla et al, 2009).…”

Efecto de la salinidad en la germinación y emergencia de siete especies forrajeras

“…At 200 mM K + content of species was reduced from 25.72 to 52.11%; being H. vulgare and L. perenne the species with less reduction, the range between 5.74 and 16.73% respectively. The concentration of K + is lower in plants stressed by salinity compared to treatments without stress during the evaluation cycle, suggesting that potassium is the major mechanism of tolerance to salt stress, by its ability to maintain cell turgor, osmotic adjustment and growth (Sobhanian et al, 2010).…”

“…A 200 mM, el contenido de K + de las especies se vio reducido de 25.72 a 52.11%; siendo H. vulgare y L. perenne las especies que mostraron una menor reducción, al oscilar entre 5.74 y 16.73% respectivamente. La concentración de K + es más baja en plantas estresadas por salinidad comparada con los tratamientos sin estrés durante el ciclo de evaluación, lo cual sugiere que el potasio constituye el principal mecanismo de tolerancia al estrés salino, por su capacidad de mantener la turgencia celular, el ajuste osmótico y el crecimiento (Sobhanian et al, 2010).…”

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

“…Adenosine triphosphate (ATP) is vital for many biosynthetic pathways in plant cells, and energy requirements may increase considerably during periods of external stress. Adenosine kinase and ATP synthase are up-regulated by salinity in wheat (Wang et al, 2008), rice (Kim et al, 2005), potato (Aghaei et al, 2008), and the C4 plant Aeluropus lagopoides (Poaceae) (Sobhanian et al, 2010a). This shows that the maintenance of ATP-dependent salt tolerance by increasing the formation of ATP is one strategy that plants adopt to cope with salt stress.…”