Salt Stress Tolerance of Methylotrophic Bacteria Methylophilus sp. and Methylobacterium sp. Isolated from Coal Mine Spoils

Giri, Deen Dayal; Kumar, Ajay; Shukla, Prabhu Nath; Singh, Ranjeet; Singh, Pratika; Pandey, Kapil Deo

doi:10.33073/pjm-2013-035

Cited by 17 publications

(6 citation statements)

References 41 publications

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“…The remaining OTUs were Methylophilus (0.5-5.9%), Sulfuricurvum (0.6-14.3%), and Haemophilus (1.7-3.8%). All of these have also been found previously in coal mines: Haemophilus [56]; Methylobacter [57]; Methylomonas [58]; Methylophilus [59]; Methylotenera [60]; Sulfuricurvum [61]. The above OTUs corresponded to seven bacterial phyla out of which Proteobacteria dominated all samples (Figure 6).…”

Section: Microbial Diversitysupporting

confidence: 82%

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

et al. 2021

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During long periods with no precipitation, a sulfidic spring (Smrdljivec) appears in the dry bed of the Reka River before sinking into the karst underground. The study characterizes the area’s geological setting, development of microbial communities and an ecotone, and impact on the vulnerable karst ecosystem. Geological mapping of the area, stable isotopic analyses, field measurements, and physico-chemical and toxicity analyses were applied to elucidate the environmental conditions. The spring’s microbial diversity was assessed using cultivation methods, microscopy, and metagenomics. Sulfur compounds in the spring probably originate from coal layers in the vicinity. Metagenomic analyses revealed 175 distinct operational taxonomic units in spring water and biofilms. Proteobacteria predominated in developed biofilms, and a “core” microbiome was represented by methylotrophs, including Methylobacter, Methylomonas, and Methylotenera. Diatoms represented an important component of biofilm biomass. A combination of environmental factors and climatic conditions allows the formation and accessibility of emerging biodiversity hotspots and ecotones. Details of their dynamic nature, global impact, and distribution should be highlighted further and given more protection.

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Section: Microbial Diversitysupporting

confidence: 82%

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

et al. 2021

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“…and Methylobacterium sp. were reported to grow in the presence of NaCl with Methylobacterium showing increased enzyme activity even at 500 mM NaCl concentration (Giri et al 2013 ). Performance of aggregated and non-aggregated cells in this study even at 200 mM concentration substantiates the ability of Methylobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development

et al. 2017

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Salinity is one of the major factors contributing to the loss of crop productivity and thereby impacting livelihood of people in more than 100 countries of the world and the area of land affected by salinity is increasing day by day. This will worsen due to various factors such as drought that might result in high soil salinity. Use of plant growth promoting rhizobacteria is one of the promising eco-friendly strategies for salinity stress management as part of sustainable agricultural practices. However, it requires selecting rhizobacteria with good survivability and adaptation to salt stress. In this study we report aggregation of Methylobacterium oryzae CBMB20 cells grown in media containing high C/N ratio (30:1) than in media containing low C/N ratio (7:1). Aggregated Methylobacterium oryzae CBMB20 cells exhibited enhanced tolerance to UV irradiation, heat, desiccation, different temperature regimes, oxidative stress, starvation and supported higher population in media. Poly-β-hydroxybutyrate accumulation, exopolysaccharide production, proline accumulation and biofilm formation were good at 100 mM salt concentration with good microbial cell hydrophobicity at both 50 and 100 mM than other concentrations. Both the aggregated and non-aggregated cells grown under 0–200 mM salt concentrations produced IAA even at 200 mM salt concentration with a peak at 100 mM concentration with aggregated cells producing significantly higher quantities. ACC deaminase activity was observed in all NaCl concentrations studied with gradual and drastic reduction in aggregated and non-aggregated cells over increased salt concentrations.

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“…These can also be catabolized to enhance the accumulation of other osmoprotectants. Some of the sugar alcohols (polyols) include glycerol, inositol, mannitol, sorbitol, arabitol and maltitol ( 10 , 11 ). For detailed reviews of the different classes of osmoprotectants and their mode of action, we refer the reader to published reviews ( 3 , 4 , 11–16 ).…”

Section: Introductionmentioning

confidence: 99%

DEOP: a database on osmoprotectants and associated pathways

et al. 2014

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Microorganisms are known to counteract salt stress through salt influx or by the accumulation of osmoprotectants (also called compatible solutes). Understanding the pathways that synthesize and/or breakdown these osmoprotectants is of interest to studies of crops halotolerance and to biotechnology applications that use microbes as cell factories for production of biomass or commercial chemicals. To facilitate the exploration of osmoprotectants, we have developed the first online resource, ‘Dragon Explorer of Osmoprotection associated Pathways’ (DEOP) that gathers and presents curated information about osmoprotectants, complemented by information about reactions and pathways that use or affect them. A combined total of 141 compounds were confirmed osmoprotectants, which were matched to 1883 reactions and 834 pathways. DEOP can also be used to map genes or microbial genomes to potential osmoprotection-associated pathways, and thus link genes and genomes to other associated osmoprotection information. Moreover, DEOP provides a text-mining utility to search deeper into the scientific literature for supporting evidence or for new associations of osmoprotectants to pathways, reactions, enzymes, genes or organisms. Two case studies are provided to demonstrate the usefulness of DEOP. The system can be accessed at.Database URL: http://www.cbrc.kaust.edu.sa/deop/

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Salt Stress Tolerance of Methylotrophic Bacteria Methylophilus sp. and Methylobacterium sp. Isolated from Coal Mine Spoils

Cited by 17 publications

References 41 publications

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

Emerging Ecotone and Microbial Community of a Sulfidic Spring in the Reka River near Škocjanske Jame, Slovenia

Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development

DEOP: a database on osmoprotectants and associated pathways

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