Salt tolerance and glycerol accumulation of a respiration-deficient mutant isolated from the petite-negative, salt-tolerant yeast Zygosaccharomyces rouxii

Abstract

A respiration-deficient (RD) mutant was isolated from the petite-negative, salt-tolerant yeast Zygosaccharomyces rouxii. One strain among sixteen glycerol-non-utilizing mutants exhibited vigorous liberation of CO2 but no uptake of O2. Furthermore, this strain lacked cytochrome aa3 and had a reduced level of cytochrome b. The few mitochondria found in cells of this strain contained few or no cristae. Salt tolerance and intracellular accumulation of glycerol by the RD strain were almost equal to that of the wild-type strain in media containing NaCl up to 2.5 M. In media with more than 3 M NaCl, the growth of the RD mutant was retarded and the intracellular accumulation of glycerol was depressed in spite of ample production.

The yeast : a new host for heterologous protein production, secretion and for metabolic engineering applications

“…Starting from the early 1980s, the majority of recombinant proteins produced in yeasts have been expressed using Saccharomyces cerevisiae [1]. While the main property of the yeast Z. rouxii is its salt tolerance [15,16], Z. bailii can tolerate high sugar concentrations as well as acidic environments, relatively high temperatures and can survive in the presence of high concentrations of chemical preservatives [17^22]. Furthermore, S. cerevisiae is an organism generally regarded as safe.…”

Production of 1,3-propanediol by Klebsiella pneumoniae using raw glycerol from Zygosacharomyces rouxii

“…rouxii JL2011 and K. pneumoniae were stocked in our laboratory. The fermentation experiments with Z. rouxii JL2011 were carried out as described by Yagi et al (1992). K. pneumoniae was used for 1,3-PD production from glycerol.…”

Intermediary Metabolism

“…Glycerol production is often correlated with osmotic stress and many fungi appear to rely at least partially on glycerol for turgor restoration (Beever and Laracy, 1986;Val). Laere and Hulsmans, 1987;Andre et a/., 1988;Bellinger and Larher, 1988;Yagi et a/., 1992). Glycerol production in Phycomyces spores is controlled by the activity of a specific glycerol-3-phosphatase.…”