Salt tolerance of barley: Relations between expression of isoforms of vacuolar Na+/H+-antiporter and 22Na+ accumulation

Roslyakova, T. V.; Молчан, О. В.; Vasekina, A. V.; Лазарева, Е. М.; Соколик, Анатолий Иосифович; Yurin, Vladimir; Boer, Albertus H. de; Бабаков, А. В.

doi:10.1134/s1021443711010158

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…In plants grown in salt conditions, over‐accumulation of Na + and/or Cl + ions leads to inhibition of the Na + /H + antiporters localized in the plasma membrane, resulting ion toxicity and osmotic stress (Roslyakova et al . 2011). In the present study, the pea genotype used contained large amounts of Na + ions but reduced K + ions in leaves and roots under salinity stress.…”

Section: Discussionmentioning

confidence: 99%

Seed priming with gibberellic acid induces high salinity tolerance in Pisum sativum through antioxidants, secondary metabolites and up‐regulation of antiporter genes

et al. 2020

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Salinity is one of the major abiotic stresses that limit productivity of pulse crops all over the world. Seed priming with phytohormone(s) is one of the most promising, authentic and cost-effective methods to mitigate the deleterious effect of salinity.• The study was conducted to investigate potential of seed priming with gibberellic acid (GA 3 ) to cope up with the adverse effects of salinity (0, 100, 200 and 300 mM NaCl) in pea (Pisum sativum L.) seedlings.• There were different responses to salinity, which induced oxidative stress, higher accumulation of Na + in shoots and roots and inhibition of photosynthetic traits. However, seed priming with GA 3 showed promising effects on physiological traits under salinity stress and alleviated the adverse effects of salinity by inducing the antioxidant system, proline production, total phenol and flavonoid content and regulating ion homeostasis, along with up-regulation of Na + /H + antiporters (SOS1 and NHX1).• Plants adapt and prevent high salt accumulation by inducing expression of Na + / H + antiporter (SOS1 and NHX1) proteins that enhance Na + sequestration. Thus, seed priming with GA 3 is important in alleviation of high salinity stress and can be used as a criterion for developing salt-tolerant cultivars.

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Section: Discussionmentioning

confidence: 99%

Seed priming with gibberellic acid induces high salinity tolerance in Pisum sativum through antioxidants, secondary metabolites and up‐regulation of antiporter genes

et al. 2020

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“…/H ? -antiporters operating normally at the plasma membrane (Roslyakova et al 2011). Sodium uptake is mediated by both voltage-dependent and -independent cation channels.…”

Section: Discussionmentioning

confidence: 99%

Effects of ascorbic acid and gibberellin A3 on alleviation of salt stress in common bean (Phaseolus vulgaris L.) seedlings

et al. 2012

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Salinity, a severe environmental factor, has limited the growth and productivity of crops. Many compounds have been applied to minimize the harmful effects of salt stress on plant growth. An experiment was conducted to investigate the interactive effects of exogenous ascorbic acid (AsA) and gibberellic acid (GA 3 ) on common bean (Phaseolus vulgaris L. cv. Naz) seedlings under salt stress. The changes of growth parameters, photosynthetic and non-photosynthetic pigments and potassium content showed that the addition of 1 mM AsA and/or 0.05 mM GA 3 considerably decreased the oxidative damage in common bean plants treated with 200 mM NaCl. The NaCl-stressed seedlings exposed to AsA or GA 3 , specifically in their combination, exhibited an improvement in sodium accumulation in both roots and shoots, as compared to NaCl-treated plants. NaCl treatment increased hydrogen peroxide (H 2 O 2 ) content and lipid peroxidation indicated by accumulation of malondialdehyde (MDA), whereas the interaction of AsA with GA 3 decreased the amounts of MDA and H 2 O 2 . In the meantime, interactive effect of these substances enhanced protein content and the activity of the antioxidant enzyme, guaiacol peroxidase, in common bean plants under salt stress. It was concluded that synergistic interaction between AsA and GA 3 could alleviate the adverse effects of salinity on P. vulgaris seedlings.

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“…Transcription of HvNHX1 which encodes a vacuolar antiporter, has been previously investigated in barley under SS (Roslyakova et al 2011): an increase in HvNHX1 transcription and protein content has been demonstrated but not in transcription of HvNHX2 and 3. In this work, we observed a similar induction of HvNHX1 in the SS and HS treated plants in terms of timing; however, the transcription increase was more pronounced in the HS treated seedlings.…”

Section: Discussionmentioning

confidence: 99%

“…A metallothionein-like protein type 2 (HvMT2) has been reported to be highly transcribed in barley leaves and roots after exposure to 150 mM NaCl (Öztürk et al 2002). The NHX1 gene coding for Na + /H + antiport at the tonoplast plays an important role in maintaining K + homeostasis by extruding Na + from the cytosol into the vacuole during salt stress (Roslyakova et al 2011, Bassil et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Effects of heat shock and salinity on barley growth and stress-related gene transcription

Faralli¹,

Lektemur²,

Rosellini³

et al. 2015

Biologia plant.

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The effects of a short (30 min) heat shock (HS) on plants subsequently grown under a salinity stress (SS, 200 mM NaCl) for 10 d were investigated in barley (Hordeum vulgare L.) cv. Tokak 157/37. The maximum temperature for HS allowing plant survival was 45 °C. The root length was significantly decreased by SS, whereas HS alone did not affect root growth. Interestingly, HS stimulated root elongation under SS. An osmotic adjustment was promoted in leaves by SS. On the contrary, HS increased the osmotic potential in leaves in the absence of SS, and partly counteracted the effect of SS in the HS+SS treatment. Cu/Zn-SOD, HvAPX, HvCAT2, HSP17, HSP18, and HSP90 were transcribed in leaves of HS-treated plants, but not in control plants. The HSP70 was constitutively transcribed in both the SS and control plants, but after HS, a shorter amplicon was also observed. The genes coding antioxidants, Cu/Zn-SOD, HvCAT2 and HvAPX, were differentially influenced by SS or HS+SS in the roots and leaves. In the roots, the mRNA content of BAS1, HvDRF1, HvMT2, and HvNHX1 increased after the HS treatment. In a recovery experiment in which plants were grown to maturity after HS and HS+SS stress exposure, the plant height increased and the time to maturity was reduced in comparison with SS. Our results show that HS could stimulate plant growth and reduce some of the negative effects of SS, and that it affected the transcription of several stress-related genes.

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Salt tolerance of barley: Relations between expression of isoforms of vacuolar Na+/H+-antiporter and 22Na+ accumulation

Cited by 8 publications

References 23 publications

Seed priming with gibberellic acid induces high salinity tolerance in Pisum sativum through antioxidants, secondary metabolites and up‐regulation of antiporter genes

Seed priming with gibberellic acid induces high salinity tolerance in Pisum sativum through antioxidants, secondary metabolites and up‐regulation of antiporter genes

Effects of ascorbic acid and gibberellin A3 on alleviation of salt stress in common bean (Phaseolus vulgaris L.) seedlings

Effects of heat shock and salinity on barley growth and stress-related gene transcription

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