Salt tolerance of transgenic rice (Oryza sativa L.) withmtlD gene andgutD gene

Wang, Huizhong; Danian, Huang; Lu, Ruifang; Liu, Junjun; Qian, Qian; Xuexian, Peng

doi:10.1007/bf02898987

Cited by 13 publications

(3 citation statements)

References 14 publications

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“…Under osmotic stress (salt- and water-stress driven) mannitol accumulation is attributable to a reduction in the catabolism of mannitol in green tissues ( Stoop and Mooibroek, 1998 ). Mannitol production induced by water stress has been widely observed in plant species, e.g., tomatoes ( Wang et al, 2000 ), sugarcane ( Cha-um and Kirdmanee, 2008 ), rice, and sorghum ( Cha-um et al, 2009 ).…”

Section: Accumulation Of Mannitol In Plantsmentioning

confidence: 99%

Mannitol metabolism during pathogenic fungal–host interactions under stressed conditions

et al. 2015

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Numerous plants and fungi produce mannitol, which may serve as an osmolyte or metabolic store; furthermore, mannitol also acts as a powerful quencher of reactive oxygen species (ROS). Some phytopathogenic fungi use mannitol to stifle ROS-mediated plant resistance. Mannitol is essential in pathogenesis to balance cell reinforcements produced by both plants and animals. Mannitol likewise serves as a source of reducing power, managing coenzymes, and controlling cytoplasmic pH by going about as a sink or hotspot for protons. The metabolic pathways for mannitol biosynthesis and catabolism have been characterized in filamentous fungi by direct diminishment of fructose-6-phosphate into mannitol-1-phosphate including a mannitol-1-phosphate phosphatase catalyst. In plants mannitol is integrated from mannose-6-phosphate to mannitol-1-phosphate, which then dephosphorylates to mannitol. The enzyme mannitol dehydrogenase plays a key role in host–pathogen interactions and must be co-localized with pathogen-secreted mannitol to resist the infection.

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Section: Accumulation Of Mannitol In Plantsmentioning

confidence: 99%

Mannitol metabolism during pathogenic fungal–host interactions under stressed conditions

et al. 2015

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“…The mannitol dehydrogenase (MTLD) enzyme, encoded by the bacterial mtlD gene, is the key enzyme in mannitol metabolism, reversibly converting fructose-6-phosphate to mannitol-1-phosphate. The mtlD gene has been transferred to several crop species, resulting in certain cases in enhanced plant height, fresh and dry biomass weight, increase in salinity and/or drought tolerance, and often in accumulation of mannitol [32][33][34][35][36][37][38][39][40][41][42]. Research on transfer of bacterial RNA chaperones performed to induce abiotic stress tolerance in maize is among other promising research areas [43].…”

Section: Introductionsmentioning

confidence: 99%

Transgene Pyramiding of theHVA1andmtlDin T3 Maize (Zea maysL.) Plants Confers Drought and Salt Tolerance, along with an Increase in Crop Biomass

Nguyen

Alameldin

et al. 2013

International Journal of Agronomy

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The pBY520 containing the Hordeum vulgare HVA1 regulated by the rice actin promoter (Act1 5 ) or the JS101 containing the bacterial mannitol-1-phosphate dehydrogenase (mtlD) also regulated by rice Act1 5 and a combination of these two plasmids were transferred into the maize genome, and their stable expressions were confirmed through fourth generations. Plants transcribing a combination of the HVA1+mtlD showed higher leaf relative water content (RWC) and greater plant survival as compared with their single transgene transgenic plants and with their control plants under drought stress. When exposed to various salt concentrations, plants transcribing the HVA1+mtlD showed higher fresh and dry shoot and dry root matter as compared with single transgene transgenic plants and with their control plants. Furthermore, the leaves of plants expressing the mtlD accumulated higher levels of mannitol. Plants expressing the HVA1+mtlD improved plant survival rate under drought stress and enhanced shoot and root biomass under salt stress when compared with single transgene transgenic plants and with their wild-type control plants. The research presented here shows the effectiveness of coexpressing of two heterologous abiotic stress tolerance genes in the maize genome. Future field tests are needed to assure the application of this research.

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“…Now, transgenic plants, which were cultivated by the techniques of gene engineering, can tolerate high salt stress and show a prospective future. Some genes encoding membrane transporter and biosynthesis of the organic salt-tolerant osmotica have been cloned, such as bataine-aldehyde dehydrogenase gene (BADH) [1] , yeast K + /Na + transporter gene [2] , levansucrase gene [3] , 1-phophate mannitol dehydrogenase gene (mtl D) [4,5] , 6-phosphate osmolyte mannitol dehydrogenase gene (gut D) [5] , and so on. These genes have been successfully transferred into tobacco, tomato, wheat, rice, maize, watercress and other crops [1 8] to improve the ability of salt tolerance.…”

mentioning

confidence: 99%

Transformation of the salt-tolerant gene of Avicennia marina into tobacco plants and cultivation of salt-tolerant lines

ZHOUHantao¹,

LINQingtong²,

PANWen³

et al. 2004

Chinese Sci Bull

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Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Km r and Hyg r could be transferred into tobacco genome by the ameliorated leaf discs method of agrobacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosynthetic rates indicated that the survival rate of the transgenic lines is 80% 90% and the transgenic lines could increase by 30% 40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na + stress, but also to the comprehensive stress of various ions.Soil salinization is an important factor in confining the development of agriculture. There are about 380 million hectare saline lands all over the world, and one third of irrigated areas are affected by high salinity. 2.17 million hectare tidal flats are along the coasts of China, with 300 hundred hectare increasing each year. There are 37 million hectare saline lands in Chinese inlands, which take about 20% of cultivable areas. Most of crops in the world cannot tolerate high salt stress, and they could not be planted in the tidal flats along the coasts or saline lands. With the increasing of population, decreasing of plantation, and seriously lacking of fresh water, the cultivation of salttolerant crops to sustain the development of agriculture is the first magnitude in the modern biotechnology.In recent years, there are lots of studies on the molecular mechanism of salt tolerance of plants. Many proteins have been found, which include enzymes to synthesize small osmotic molecules, preservative proteins, enzymes relevant to absorbing, transferring and locating salt in the cell, and so on. Now, transgenic plants, which were cultivated by the techniques of gene engineering, can tolerate high salt stress and show a prospective future. Some genes encoding membrane transporter and biosynthesis of the organic salt-tolerant osmotica have been cloned, such as bataine-aldehyde dehydrogenase gene (BADH) [1] , yeast K + /Na + transporter gene [2] , levansucrase gene [3] , 1-phophate mannitol dehydrogenase gene (mtl D) [4,5] , 6-phosphate osmolyte mannitol dehydrogenase gene (gut D) [5] , and so on. These genes have been successfully transferred into tobacco, tomato, wheat, rice, maize, watercress and other crops [1 8] to improve the ability of salt tolerance. The pr...

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Salt tolerance of transgenic rice (Oryza sativa L.) withmtlD gene andgutD gene

Cited by 13 publications

References 14 publications

Mannitol metabolism during pathogenic fungal–host interactions under stressed conditions

Mannitol metabolism during pathogenic fungal–host interactions under stressed conditions

Transgene Pyramiding of theHVA1andmtlDin T3 Maize (Zea maysL.) Plants Confers Drought and Salt Tolerance, along with an Increase in Crop Biomass

Transformation of the salt-tolerant gene of Avicennia marina into tobacco plants and cultivation of salt-tolerant lines

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