Salt tolerant SUV3 overexpressing transgenic rice plants conserve physicochemical properties and microbial communities of rhizosphere

Sahoo, Ranjan Kumar; Ansari, Mohammad Wahid; Tuteja, Renu; Tuteja, Narendra

doi:10.1016/j.chemosphere.2014.08.011

Cited by 16 publications

(15 citation statements)

References 33 publications

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“…Both constructs, with or without the MTS, drive the growth rate above the control level. Interestingly, similar effects were observed in plants, where overexpression of the fulllength wild-type plant SUV3 gene resulted in improved stress resistance in rice, delayed leaf senescence-associated events and resulted in 3.5-fold increase in the telomere length (Sahoo et al, 2014;Sahoo et al, 2015;Macovei et al, 2016).…”

Section: Resultsmentioning

confidence: 57%

Human SUV3 helicase in the cell nucleus regulates the growth rate of HeLa cells.

et al. 2017

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The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle.

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Section: Resultsmentioning

confidence: 57%

Human SUV3 helicase in the cell nucleus regulates the growth rate of HeLa cells.

et al. 2017

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“…The overexpression of PDH45 and SUV3 in transgenic rice has no adverse effect on rhizosphere soil or its microflora [3, 7]. In other studies of transgenic crops, the only consistent significant differences in soil enzymes and physicochemical properties between transgenic and nontransgenic plants were due to seasons and crop varieties.…”

Section: Resultsmentioning

confidence: 99%

“…No significant effects were found on the populations of various soil microorganisms with the growth of transgenic insect-resistant maize, Bt maize, and cotton compared to nontransgenic plants under field conditions [30, 31, 35–37]. There was no adverse effect on soil enzymatic activities or rhizosphere microbial communities by the cultivation of transgenic plants, such as MCM6 transgenic tobacco, PDH45 transgenic rice, and SUV3 -overexpressing transgenic rice [3, 7, 37]. …”

Section: Resultsmentioning

confidence: 99%

“…No significant differences in the isolated rhizospheres were found during plant growth in GM or non-GM plants [6]. Using transgenic, salinity-tolerant SUV3 and PDH45 rice, the communication between rhizobacteria and rice was studied, and no significant effect was found [3, 7]. However, there have been few reports of the influence of GM and non-GM maize on rhizosphere soils [8].…”

Section: Introductionmentioning

confidence: 99%

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Effect on Soil Properties ofBcWRKY1Transgenic Maize with Enhanced Salinity Tolerance

Zeng

Zhou

Zhu

et al. 2016

International Journal of Genomics

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Maize (Zea mays L.) is the most important cereal crop in the world. However, soil salinity has become a major problem affecting plant productivity due to arable field degradation. Thus, transgenic maize transformed with a salinity tolerance gene has been developed to further evaluate its salt tolerance and effects on agronomic traits. It is necessary to analyze the potential environmental risk of transgenic maize before further commercialization. Enzyme activities, physicochemical properties, and microbial populations were evaluated in saline and nonsaline rhizosphere soils from a transgenic maize line (WL-73) overexpressing BcWRKY1 and from wild-type (WT) maize LH1037. Measurements were taken at four growth stages (V3, V9, R1, and R6) and repeated in three consecutive years (2012–2014). There was no change in the rhizosphere soils of either WL-73 or WT plants in the four soil enzyme activities, seven soil physicochemical properties, and the populations of three soil organisms. The results of this study suggested that salinity tolerant transgenic maize had no adverse impact on soil properties in soil rhizosphere during three consecutive years at two different locations and provided a theoretical basis for environmental impact monitoring of salinity tolerant transgenic maize.

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“…To evaluate the potential risks of transgenic plants on soil bacteria and other undergrowth vegetation, the number of the rhizosphere microbe community and allelopathic activity of transgenic plants must be measured because foreign gene expression could change the released substances of the receptor plants, which may impact the rhizosphere microbe community or the growth of undergrowth vegetation [16]. Although some field trials of transgenic plants have shown no significant difference on the microbial communities or allelopathic activity between transgenic and non-transgenic plants [1,[16][17][18], Fang et al [19] found inhibiting the expression of a Phenylalanine Ammonia-lyase by RNAi in rice changed the allelopathy and rhizosphere microflora. Beckers et al [20] thought the rhizosphere microflora changes might be due to changes of metabolism in transgenic plants.…”

Section: Introductionmentioning

confidence: 99%

An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

Guo

Luo

et al. 2018

Forests

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Populus tomentosa grow rapidly, but are salt susceptible. To quickly and efficiently gain new poplar breeds with better salt resistance, a DREB transcription factor derived from Atriplex hortensis was transformed into triploid Populus tomentosa by our lab, which significantly improved the salt tolerance of host plants. However, environmental impacts of transgenic plants must be assessed before large-scale cultivation in China. Here, we conducted a field trial of AhDREB1 transgenic and non-transgenic triploid Populus tomentosa to assess the impact of transgenic trees on rhizospheric soil microbial communities and allelopathic activity of leaves. No significant differences in the number of soil microbes present were detected between the transgenic lines and the non-transgenic controls. The allelopathic activity of leaves from both the transgenic and non-transgenic lines varied with sampling time, but did not differ significantly between the transgenic and non-transgenic lines. These results indicate that the impact on the environment of AhDREB1 transgenic P. tomentosa did not differ significantly from that of the non-transformed controls for the variables observed in this field trial. We also investigated the persistence of AhDREB1 genes in decomposing transgenic poplar leaf on the soil under natural conditions for five months, and our data indicated that fragments of the genetically modified DNA were not detectable in the field after more than two months. We used a triphenyl tetrazolium chloride test (TTC) (or pollen germination method) and hybridization to test the pollen viability and fertility, respectively, of the transgenic and non-transgenic trees and the results showed that the pollen viability of both the transgenic and non-transgenic trees was extremely low in 2016; the receptor plant may have been sterile.

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Salt tolerant SUV3 overexpressing transgenic rice plants conserve physicochemical properties and microbial communities of rhizosphere

Cited by 16 publications

References 33 publications

Human SUV3 helicase in the cell nucleus regulates the growth rate of HeLa cells.

Human SUV3 helicase in the cell nucleus regulates the growth rate of HeLa cells.

Effect on Soil Properties ofBcWRKY1Transgenic Maize with Enhanced Salinity Tolerance

An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

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