Salting-out assisted liquid–liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples

Sazali, Nur Hidayah; Alshishani, Anas; Saad, Bahruddin; Chew, Ker Yin; Chong, Moi Me; Miskam, Mazidatulakmam

doi:10.1098/rsos.190952

Cited by 30 publications

(20 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6), which indicated that the extraction method influenced the salting-out efficiency of solamargine and solasonine from the aqueous solution in the presence of NaCl or Na 2 CO 3 . The effect of extraction conditions on salting-out was also reported by Tajeda-Casado et al [20] and Sazali et al [21]. The doubly charged carbonate ions from Na 2 CO 3 interacted with the hydration sphere surrounding the solute (solamargine and solasonine) to a greater extent than singly charged chloride ions from NaCl, forming carbonic acid.…”

Section: Ma-atpesupporting

confidence: 67%

“…Aqueous two-phase extraction (ATPE) is desired for its environmental compatibility, low interfacial tension of phases, high yields, and low process time [17,18]. Recently, researchers have turned their attention to an improved version of ATPE, salting-out assisted liquid-liquid extraction (SALLE) technique, which facilitates extraction of metabolites from complex matrices [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyphenation of aqueous two-phase and microwave extraction of solasonine and solamargine from leaves of Solanum mauritianum characterized by UHPLC-qTOF-MS

Mokgehle

Madala

Gitari

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

The biomass Solanum mauritianum (S. mauritianum) is an invasive weed specie; however, it is a source of medicinally important metabolites, as reported in literature, such as solasonine and solamargine. The study was directed at the optimization of microwave and aqueous two-phase-based extraction techniques which involved microwave-assisted extraction (MAE), aqueous two-phase extraction followed by microwave-assisted extraction (ATPE + MAE), and the “one-pot” microwave-assisted aqueous two-phase extraction (MA-ATPE) for extraction of solasonine and solamargine from leaves of S. mauritianum which was evaluated. The microwave-assisted extraction of solasonine and solamargine yielded optimums at 5.00 min, microwave power of 270 W, and solid/liquid of 0.1 g L−1 at an ethanol concentration of 60%. Application of a two-stage extraction (MAE + ATPE) in CaO-dried alcohol resulted in decreased amounts of solasonine and solamargine extracted. The best yields of solasonine and solamargine were achieved in the MA-ATPE method. Extraction of solamargine and solasonine using Na2CO3 in CaO-dried ethanol during MA-ATPE was approximately threefold and twofold greater than that of MAE + ATPE, respectively. Furthermore, extraction of solamargine and solasonine using NaCl in CaO-dried ethanol during MA-ATPE was approximately twofold greater than that of MAE + ATPE. The synergy of microwaves and salting-out in the “one-pot” MA-ATPE technique was shown to be a contributing factor for enhanced extraction of solamargine and solasonine from leaves of S. mauritianum. Application of this time- and energy-efficient extraction method could potentially be expanded for enrichment of medicinally important compounds from biomass of other medicinal plants.

show abstract

Section: Ma-atpesupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Hyphenation of aqueous two-phase and microwave extraction of solasonine and solamargine from leaves of Solanum mauritianum characterized by UHPLC-qTOF-MS

Mokgehle

Madala

Gitari

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

show abstract

“…Furthermore, the extent of salting out was further driven by the increase in mass of plant powder due to mass transfer under the conditions studied. The salting-out effect in ATPE was also reported by Sazali et al (2019) and Mokgehle et al (2021). Additionally, the higher extraction of solasodine via Na 2 CO 3 compared to NaCl can correlates with the Hoffmeister series.…”

Section: Chromatographic Pro Le Of Mrm Based Quanti Cation Of Solasodinesupporting

confidence: 57%

Optimization in the Aqueous Two Phase Extraction of a Toxic Metabolite, Solasodine, From S. Mauritianum and Analysis via UHPLC-qTOF-MS

Mokgehle

Madala

Gitari

et al. 2021

Preprint

View full text Add to dashboard Cite

Aqueous two phase extraction (ATPE) was applied in the extraction of an allelochemical, solasodine, from an invasive plant, Solanum mauritianum. Central composite design was performed which included numerical parameters such as time and mass of plant powder. The categorical factors included the type of salt used in aiding extraction such as the chaotrope (NaCl) and kosmotrope (Na2CO3). Fitting the central composite design response surface model to the experimental data generated a quadratic model with a good fit (R2 = 0.925). The linear effect of mass of plant powder was a statistically significant (p < 0.05) parameter for solasodine extraction. The optimized conditions for the extraction of solasodine in the presence of NaCl or Na2CO3 were time: 10 min and mass of plant powder: 1.2 g. Corresponding to these conditions, the maximal mean extraction based on multiple reaction monitoring (MRM) transition of solasodine (m/z 414 → 396) on the UHPLC-qTOF-MS was 233.65 mg kg-1 and 413.50 mg kg-1 for NaCl and Na2CO3, respectively. The greater extraction ability of the kosmotrope was due to the higher negative charge density of the carbonate ion during salting-out. Furthermore, the synergistic effect of mass of plant powder and salting-out was shown to enhance extraction of solasodine compared to the chaotrope. The kosmotrope assisted solasodine ATPE extracts from Solanum mauritianum, can potentially be applied as antipathogenic agents in medicine while simultaneously limiting the allelopathic impact of Solanum mauritianum.

show abstract

“…Salting-out is an effective pre-concentration method which has been studied for the extraction of Vitamin D3 in milk samples [26] partitioning of biomolecules [56] and determination of 5-nitroimidazolesin in fish [21]. Salting-out is dependent on the nature of the salt involved.…”

Section: Mass Spectra Of Alkaloid Derivativesmentioning

confidence: 99%

“…This method uses solvents such as ethanol (EtOH) or acetonitrile (MeCN), as extraction solvents because water-solubility is minimized when salts are added to samples and extraction media [20,21]. The SALLE procedure has been widely applied for extraction of compounds from various matrices because of its simplicity and effectiveness [21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Deciphering the effects of kosmotrope and chaotrope salts during aqueous two phase extraction (ATPE) of polyphenolic compounds and glycoalkaloids from the leaves of a nutraceutical plant, Solanum retroflexum, with the aid of UPLC-QTOF-MS

et al. 2021

View full text Add to dashboard Cite

Solanum plants (Solanaceae) are renowned source of nutraceuticals and have widely been explored for their phytochemical constituents. This work investigated the effects of kosmotropic and chaotropic salts on the number of phytochemicals extracted from the leaves of a nutraceutical plant, Solanum retroflexum, and analyzed on the ultra-performance liquid chromatography hyphenated to a quadrupole time of flight mass spectrometer (UPLC-QTOF-MS) detector. Here, a total of 20 different compounds were putatively characterized. The majority of the identified compounds were polyphenols and glycoalkaloids. Another compound, caffeoyl malate was identified for the first time in this plant. Glycoalkaloids such as solanelagnin, solamargine, solasonine, β-solanine (I) and β-solanine (II) were found to be extracted by almost all the salts used herein. Kosmotrope salts, overall, were more efficient in extracting polar compounds with 4 more polyphenolic compounds extracted compared to the chaotropes. Chaotropes were generally more selective for the extraction of less polar compounds (glycoalkaloids) with 3 more extracted than the kosmotropes. The chaotrope and the kosmotrope that extracted the most metabolites were NaCl and Na2SO4, respectively, with 12 metabolites extracted for each salt. This work demonstrated that a comprehensive metabolome of S. retroflexum, more than what was previously reported on the same plant, can be achieved by application of kosmotropes and chaotropes as extractants with the aid of the Aqueous Two Phase Extraction approach. The best-performing salts, Na2SO4 or NaCl, could potentially be applied on a commercial scale, to meet the ever-growing demand of the studied metabolites. The Aqueous Two Phase Extraction technique was found to be efficient in simultaneous extraction of multiple metabolites which can be applied in metabolomics.

show abstract

Salting-out assisted liquid–liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples

Cited by 30 publications

References 32 publications

Hyphenation of aqueous two-phase and microwave extraction of solasonine and solamargine from leaves of Solanum mauritianum characterized by UHPLC-qTOF-MS

Hyphenation of aqueous two-phase and microwave extraction of solasonine and solamargine from leaves of Solanum mauritianum characterized by UHPLC-qTOF-MS

Optimization in the Aqueous Two Phase Extraction of a Toxic Metabolite, Solasodine, From S. Mauritianum and Analysis via UHPLC-qTOF-MS

Deciphering the effects of kosmotrope and chaotrope salts during aqueous two phase extraction (ATPE) of polyphenolic compounds and glycoalkaloids from the leaves of a nutraceutical plant, Solanum retroflexum, with the aid of UPLC-QTOF-MS

Contact Info

Product

Resources

About