Salusin-<b>β</b> is superior to salusin-<b>α</b> as a marker for evaluating coronary atherosclerosis

Wang, Yuxue; Wang, Songjiao; Zhang, Jun; Zhang, Maona; Zhang, Hong; Gong, Ga; Luo, Ming; Wang, Teng; Mao, Xiaogang

doi:10.1177/0300060520903868

Cited by 17 publications

(7 citation statements)

References 26 publications

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“…Also, in agreement with our results, Gao, et al indicated that salusin-α decreased MMP-2/-9 expression in aortic plaque and VSMCs and subsequently repress the development of atherosclerosis by prevention the VSMC proliferation and migration (32). MMPs have major role in stenosis, Wang et al reported the association of salusins serum level with coronary artery stenosis (33) In addition, our previous studies indicated that salusin-α decreases and salusin-β increases in ammatory interleukines(IL) -6, -8 and − 18 (mRNA and protein level) in HUVECs (21,22). IL-8 could suppress expression of TIMP-1 (34) and increases mRNA/ protein of MMP-2 and MMP-9 in endothelial cells (35).…”

Section: Discussionmentioning

confidence: 96%

WITHDRAWN: The Investigation of the Effect of Salusins on Matrix Metalloproteinases and VEGF-A in human umbilical vein endothelial cells

Esfahani

Mahmoudi

Pourjafar

et al. 2023

Preprint

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Background Salusins (α and β) have anti-atherogenic and atherogenic effects. The aim of the study was to investigate the effects of salusins on expression level and activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). Also we evaluated the effect of salusins on mRNA expression and protein level of vascular endothelial growth factor-A (VEGF-A) and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs). Methods Real-time PCR, Western blotting and Zymograpgy techniques were used. Salusin-α suppressed and Salusin-β increased expression of mRNA and enzymatic activity of MMP-2 and MMP-9, respectively. Results The protein level of VCAM-1 was reduced by Salusin-α without any effect on mRNA expression. The expression of mRNA and protein level of VCAM-1 was improved by Salusin-β. Salusins increased protein level of VEGF-A without any effect on mRNA expression. Conclusions Our findings indicated an inhibitory effect of salusin-α on MMP-2 and-9, VCAM-1, and stimulatory effects of salusin-β on these molecules. Salusins have stimulatory effect on VEGF-A. Salusins may be involved in different stages of atherosclerosis. These novel results may help to detect protein-based strategies for atherosclerotic management.

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Section: Discussionmentioning

confidence: 96%

WITHDRAWN: The Investigation of the Effect of Salusins on Matrix Metalloproteinases and VEGF-A in human umbilical vein endothelial cells

Esfahani

Mahmoudi

Pourjafar

et al. 2023

Preprint

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“…Salusin-β is a bioactive peptide that is involved in certain cardiovascular diseases and has been previously associated with abnormal lipid metabolism (16). A previous clinical study reported that serum salusin-β levels are positively associated with TG levels and the TG/HDL ratio in children and adolescents with hypertension (17).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells

Xu,

Wang,

Luo

et al. 2023

Mol Med Rep

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Salusin-β and adiponectin receptor 1 (adipoR1) serve important roles in the development of certain cardiovascular diseases and lipid metabolism. However, to the best of our knowledge, the relationship between salusin-β and adipoR1, and their underlying mechanisms of action, currently remain unclear. In the present study, lentiviral vectors designed to overexpress salusin-β or knock down salusin-β expression were used in 293T and HepG2 cells. Semi-quantitative PCR was performed to investigate the relationship between salusin-β and adipoR1 mRNA expression in 293T cells. Western blotting was used to assess the protein expression levels of adipoR1, adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC) and carnitine palmitoyl transferase 1A (CPT-1A) in transfected HepG2 cells. Simultaneously, HepG2 cells were treated with an adipoR1 inhibitor (thapsigargin) or agonist (AdipoRon) and the resultant changes in the expression levels of the aforementioned proteins were observed. Oil Red O staining and measurements of cellular triglyceride levels were performed to assess the extent of lipid accumulation in HepG2 cells. The results demonstrated that salusin-β overexpression downregulated adipoR1 expression and inhibited the phosphorylation of AMPK and ACC, which led to decreased CPT-1A protein expression. By contrast, salusin-β knockdown increased adipoR1 expression and promoted the phosphorylation of AMPK and ACC, which conversely enhanced CPT-1A protein expression. Treatment with adipoR1 agonist, AdipoRon, reversed the effects of salusin-β overexpression. In addition, salusin-β overexpression enhanced intracellular lipid accumulation in HepG2 cells induced by free fatty acid treatment. These findings highlighted the potential regulatory role of salusin-β in adipoR1-mediated signaling pathways. To conclude, the present study provided insights into the regulation of fatty acid metabolism by the liver. In particular, salusin-β may serve as a potential target for the therapeutic intervention of metabolic disorders of lipids.

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“…Evaluation of the multiple linear regression model through analysis of variance revealed F > F 0.05 , p < 0.05, and R 2 > 0.9; these findings indicated that the practical design equation had high reliability. [15][16][17] The design equation showed that, among the factors influencing Q IF-Max , L/D had a much greater impact on convection effects, compared with N/D 2 . Therefore, L/D optimization can significantly increase Q IF-Max and enhance convection effects, thus improving the MM removal performances of CRRT filters.…”

Section: A Practical Design Equation To Quantify Design Factors and C...mentioning

confidence: 99%

A practical design equation for accurate quantification of CRRT filter design factors and convection effects

Yamashita

2023

Int J Artif Organs

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Considering the increasing clinical need for continuous renal replacement therapy (CRRT), further improvement in therapeutic efficacy has become an important focus for researchers. Here, we designed nine CRRT filters with various combinations of hollow fiber packing density ( PD) and housing shape (effective hollow fiber length ( L) and inner housing diameter ( D) ratio ( L/ D ratio)) to evaluate the clearance of middle molecular uremic toxins (MMs) via simulation of an in vitro continuous veno-venous hemodialysis treatment model. We also used Doppler ultrasonography to measure the maximum internal filtration flow rate ( QIF-Max) as an aid; this approach facilitated an exploration of the impacts of various design factors on convection effects, while revealing the mechanisms influencing MM removal performance. Furthermore, we constructed a multiple linear regression model of design factors and QIF-Max, then conducted experimental verification. Finally, we proposed an accurate and practical design equation to quantify the design factors influencing CRRT filters and convection effects: [Formula: see text], where N/ D2 and L/D affect QIF-Max by 15.0% and 85.0%, respectively. This design equation was able to effectively quantify the convection effects of CRRT filters with different design factors, thereby predicting MM removal performance; this convenient design equation can support the development of CRRT-related products.

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Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis

Cited by 17 publications

References 26 publications

WITHDRAWN: The Investigation of the Effect of Salusins on Matrix Metalloproteinases and VEGF-A in human umbilical vein endothelial cells

WITHDRAWN: The Investigation of the Effect of Salusins on Matrix Metalloproteinases and VEGF-A in human umbilical vein endothelial cells

Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells

A practical design equation for accurate quantification of CRRT filter design factors and convection effects

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