2010
DOI: 10.1002/jssc.200900756
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Sample complexity reduction aids efficient detection of low‐abundant proteins from human amniotic fluid

Abstract: Working with biological fluids poses a challenge of visualizing proteins present in lower concentrations. This study describes a batch-mode chromatographic method for the fractionation of human amniotic fluid (AF). This method is easy to use with minimal sample quantity, resin volume and sample processing time. For albumin depletion, two methods were evaluated. The results demonstrated that specific depletion of albumin, using affinity-ligand-based resin, is more efficient than the conventional dye-based metho… Show more

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Cited by 7 publications
(3 citation statements)
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“…This prompted us to use the heparin to fractionate the seminal fluid proteins to uncover the glycoproteins, which would otherwise not be detected. It is important to reduce the complexity of the biofluids to detect the low abundant proteins . However, in glycoproteomics also, microheterogeneity poses a problem to detect the low abundant glycopeptides.…”
Section: Discussionmentioning
confidence: 69%
“…This prompted us to use the heparin to fractionate the seminal fluid proteins to uncover the glycoproteins, which would otherwise not be detected. It is important to reduce the complexity of the biofluids to detect the low abundant proteins . However, in glycoproteomics also, microheterogeneity poses a problem to detect the low abundant glycopeptides.…”
Section: Discussionmentioning
confidence: 69%
“…To address this complexity, numerous techniques were developed for removal of highly abundant proteins from plasma/serum [38], including chicken-derived IgY antibodies [39] or hexapeptide combinatorial ligand libraries (i.e., ProteoMiner™; Bio-Rad Laboratories, CA, USA) [40,41]. Methods for removal of highly abundant proteins from other biofluids (e.g., amniotic fluid and synovial fluid) were also described [42,43]. Arguably, immuno-depletion is considered the most effective, reliable and reproducible technique [38,4447], and is widely used in contemporary biomarker research [48–50].…”
Section: Liquid Chromatography-ms Analysis Of Human Clinical Specimenmentioning
confidence: 99%
“…Reducing complexity in proteomic analyses by way of wholly removing the most abundant proteins (i.e., depletion) is one method routinely applied to the investigation of complex mixtures, such as cellular lysates or plasma . Alternative strategies for mitigating analyte complexity include refining separation methodologies through advanced liquid chromatography and peptide fractionation techniques. Moreover, complexity reduction can be achieved through mass-spectrometry tools, such as parallel reaction monitoring (PRM) and ion mobility. ,, It is important to note, however, that these approaches may either impose limitations on throughput or prove unsuitable for the analysis of sparse analyte samples and single-cell proteomes. ,, …”
Section: Introductionmentioning
confidence: 99%