Sample management for clinical biochemistry assays: Are serum and plasma interchangeable specimens?

Lima-Oliveira, Gabriel; Monneret, Denis; Guerber, Fabrice; Guidi, Gian Cesare

doi:10.1080/10408363.2018.1499708

Cited by 47 publications

(47 citation statements)

References 140 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Their density is similar, but their composition is different. The main difference is that there are more fibrinogen and coagulation proteins in plasma [32].…”

Section: Discussionmentioning

confidence: 99%

“…Their density is similar, but their composition is different. The main difference is that there are more fibrinogen and coagulation proteins in plasma [ 32 ]. Boisclair et al [ 33 ] reported that there was a very high correlation between plasma and serum D-dimer levels ( r = 0.931, P < 0.01), but the diagnostic sensitivity was not consistent.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

D-dimer in the diagnosis of periprosthetic joint infection: a systematic review and meta-analysis

Li²,

et al. 2020

J Orthop Surg Res

View full text Add to dashboard Cite

Background: D-dimer, a coagulation-related indicator, has recently been used as a tool for the diagnosis of periprosthetic joint infection (PJI), but its reliability is uncertain. The purpose of this systematic review and metaanalysis was to explore the accuracy of D-dimer in the diagnosis of PJI after joint arthroplasty. Methods: We systematically searched the MEDLINE, EMBASE, and Cochrane databases for relevant literature about D-dimer in the diagnosis of PJI. QUADAS-2 was used to assess the risk of bias and clinical applicability of each included study. We used the bivariate meta-analysis framework to pool the sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the SROC curve (AUC). Univariate meta-regression and subgroup analyses were performed to explore the sources of heterogeneity. Results: We included 8 eligible studies. The pooled diagnostic sensitivity and specificity were 0.82 (95% CI, 0.70-0.89) and 0.70 (95% CI, 0.55-0.82), respectively. The pooled PLR, NLR, and DOR were 2.7 (95% CI, 1.7-4.4), 0.26 (95% CI, 0.15-0.46), and 10 (95% CI, 4-25), respectively. The AUC was 0.83 (95% CI, 0.8-0.86). Serum Ddimer might have higher diagnostic accuracy than plasma D-dimer for PJI (pooled sensitivity: 0.88 vs 0.67; pooled specificity: 0.76 vs 0.61). Conclusions: D-dimer has limited performance for the diagnosis of PJI.

show abstract

“…Their density is similar, but their composition is different. The main difference is that there are more fibrinogen and coagulation proteins in plasma [32].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

D-dimer in the diagnosis of periprosthetic joint infection: a systematic review and meta-analysis

Li²,

et al. 2020

J Orthop Surg Res

View full text Add to dashboard Cite

show abstract

“…One may argue that most of the analytes in this study are more stable in serum and that tubes with coagulation activators should be used when prolonged storage is needed. However, there are other disadvantages with serum compared with plasma . Plasma tubes may be centrifuged immediately, while blood in serum tubes must be allowed to rest and needs to be checked for sufficient coagulation.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are other disadvantages with serum compared with plasma. 22 Plasma tubes may be centrifuged immediately, while blood in serum tubes must be allowed to rest and needs to be checked for sufficient coagulation. In serum, there will also be a platelet dependent bias of both potassium and phosphate.…”

Section: Discussionmentioning

confidence: 99%

A comparison of stability of chemical analytes in plasma from the BD Vacutainer^® Barricor™ tube with mechanical separator versus tubes containing gel separator

Gawria

Tillmar

Landberg

2019

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background There is a need of prolonged stability of certain chemical analytes in lithium heparin tubes with separators. A new tube with a mechanical separator has recently been launched (Barricor™), which according to the manufacturer may have these benefits. The aim of this study was to evaluate stability performance of this tube in comparison with plasma gel tubes under clinically realistic circumstances. Methods Blood was collected in tubes containing lithium heparin with different separators; gel separator (Vacutainer® PST™, Becton Dickinson and Vacuette®, Greiner bio‐one) and mechanical separator (Vacutainer® Barricor™, Becton Dickinson). All tubes had an aspiration volume of 3 mL and were centrifuged at similar time and force. Tubes were transported manually or by car. Seven analytes from 122 patients were analyzed after 3 to 80 hours by Cobas c701 (Roche). Results The Barricor™ tube showed increased stability of phosphate and potassium and similar stability of aspartate aminotransferase, glucose, homocysteine, lactate dehydrogenase, and magnesium compared with gel tubes. Maximal allowable bias for phosphate was exceeded after 68 hours for Barricor™ tubes compared with 29 or 35 hours for gel tubes and for potassium after 40 hours for Barricor™ tubes vs 9 or 12 hours for gel tubes. Transportation did not affect stability. Hemolysis index was slightly lower in Barricor tubes than in gel tubes (P = .01). Conclusion Implementing the new Barricor™ tube will improve stability of potassium and phosphate in plasma. Blood sampling facilities far from the laboratory may benefit from using these tubes, thus diminishing preanalytical errors.

show abstract

“…Nucleosomes in healthy dogs are primarily released by white blood cells as they become activated or as they die. Plasma contains factors that serum does not such as clotting factors and vitamins, which may contribute to the stability of the white blood cells in the plasma samples when compared to serum [23,24]. Additionally, both EDTA and sodium citrate bind calcium as a means of preventing coagulation.…”

Section: Plos Onementioning

confidence: 99%

Evaluation of nucleosome concentrations in healthy dogs and dogs with cancer

et al. 2020

View full text Add to dashboard Cite

Introduction Nucleosomes consist of small fragments of DNA wrapped around a histone octamer core. Diseases such as cancer or inflammation lead to cell death, which causes fragmentation and release of nucleosomes into the blood. The Nu.Q™ technology measures circulating nucleosome levels and exploits the different compositions of cancer derived nucleosomes in blood to detect and identify cancer even at early stages. The objectives of this study are to identify the optimal sample type for the Nu.Q™ H3.1 assay and to determine if it can accurately detect nucleosomes in the blood of healthy canines as well as those with cancer. Materials and methods Blood samples from healthy canine volunteers as well as dogs newly diagnosed with lymphoma were used. The blood was processed at a variety of times under a variety of conditions to determine the most reliable sample type and conditions, and to develop an appropriate processing strategy to ensure reliably accurate results. Results Nucleosomes could be detected using a variety of sample collection and processing protocols. Nucleosome signals were highest in EDTA plasma and serum samples and most consistent in plasma. Samples should be processed within an hour of collection. Experiments showed that samples were able to withstand several freeze thaw cycles. Processing time and tcollection tube type did affect nucleosome detection levels. Finally, significantly elevated concentrations of nucleosomes were seen in a small cohort of dogs that had been newly diagnosed with lymphoma. Conclusions When samples are collected and processed appropriately, the Nu.Q™ platform can reliably detect nucleosomes in the plasma of dogs. Further testing is underway to validate and optimize the Nu.Q™ platform for veterinary use.

show abstract

Sample management for clinical biochemistry assays: Are serum and plasma interchangeable specimens?

Cited by 47 publications

References 140 publications

D-dimer in the diagnosis of periprosthetic joint infection: a systematic review and meta-analysis

D-dimer in the diagnosis of periprosthetic joint infection: a systematic review and meta-analysis

A comparison of stability of chemical analytes in plasma from the BD Vacutainer^® Barricor™ tube with mechanical separator versus tubes containing gel separator

Evaluation of nucleosome concentrations in healthy dogs and dogs with cancer

Contact Info

Product

Resources

About

Sample management for clinical biochemistry assays: Are serum and plasma interchangeable specimens?

Cited by 47 publications

References 140 publications

D-dimer in the diagnosis of periprosthetic joint infection: a systematic review and meta-analysis

D-dimer in the diagnosis of periprosthetic joint infection: a systematic review and meta-analysis

A comparison of stability of chemical analytes in plasma from the BD Vacutainer® Barricor™ tube with mechanical separator versus tubes containing gel separator

Evaluation of nucleosome concentrations in healthy dogs and dogs with cancer

Contact Info

Product

Resources

About

A comparison of stability of chemical analytes in plasma from the BD Vacutainer^® Barricor™ tube with mechanical separator versus tubes containing gel separator