Sample patterning on NMR surface microcoils

Ehrmann, Klaus; Gersbach, M.; Pascoal, P.; Vincent, Franck; Massin, C.; Stamou, Dimitrios; Besse, P.‐A.; Vogel, Horst; Popović, R.S.

doi:10.1016/j.jmr.2005.08.018

Cited by 24 publications

(15 citation statements)

References 23 publications

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“…A variety of planar micro-NMR detector geometries that are compatible with microfluidic devices have been proposed [5,6], including planar spiral coils [7,8], micro-slots [9], Helmholtz pairs [10], and phased array detectors [11,12].…”

Section: Introductionmentioning

confidence: 99%

An optimised detector for in-situ high-resolution NMR in microfluidic devices

Finch

Yılmaz

Utz

2016

Journal of Magnetic Resonance

109

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Integration of high-resolution nuclear magnetic resonance (NMR) spectroscopy with microfluidic lab-on-a-chip devices is challenging due to limited sensitivity and line broadening caused by magnetic susceptibility inhomogeneities. We present a novel double-stripline NMR probe head that accommodates planar microfluidic devices, and obtains the NMR spectrum from a rectangular sample chamber on the chip with a volume of 2 l. Finite element analysis is used to jointly optimise the detector and sample volume geometry for sensitivity and RF homogeneity. A prototype of the optimised design has been built, and its properties have been characterised experimentally. The performance in terms of sensitivity and RF homogeneity closely agrees with the numerical predictions. The system reaches a mass limit of detection of 1.57 nMol p s, comparing very favourably with other micro-NMR systems. The spectral resolution of this chipGprobe system is better than 1.75 Hz at a magnetic field of 7 T, with excellent line shape.

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Section: Introductionmentioning

confidence: 99%

An optimised detector for in-situ high-resolution NMR in microfluidic devices

Finch

Yılmaz

Utz

2016

Journal of Magnetic Resonance

109

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“…First, due to their relatively large fingerprint only a few planar structures can be fabricated on a single device. 19 Even in the case of microslots which have a smaller fingerprint and better ability to pack multiple detectors close together due to weaker interstructure couplings, integration of parallel detectors is extremely difficult due to the complicated electronics that would be required. Each detector would have to have its own transmitter and receiver channel which would make the technique extremely expensive and impractical.…”

Section: Direct Imagingmentioning

confidence: 99%

Magnetic resonance detection: spectroscopy and imaging of lab-on-a-chip

Harel

2009

Lab Chip

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This mini-review is focused on the use of nuclear magnetic resonance (NMR) spectroscopy and imaging to study processes on lab-on-a-chip devices. NMR as an analytical tool is unmatched in its impact across nearly every area of science, from biochemistry and medicine to fundamental chemistry and physics. The controls available to the NMR spectroscopist or imager are vast, allowing for everything from high level structural determination of proteins in solution to detailed contrast imaging of organs in-vivo. Unfortunately, the weak nuclear magnetic moment of the nucleus requires that a very large number of spins be present for an inductively detectable signal, making the use of magnetic resonance as a detection modality for microfluidic devices especially challenging. Here we present recent efforts to combat the inherent sensitivity limitation of magnetic resonance for lab-on-a-chip applications. Principles and examples of different approaches are presented that highlight the flexibility and advantages of this type of detection modality.

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“…The process is described in detail by Ehrmann et al. 4 In order to resemble a Petri dish or a well plate, a PMMA cylinder, which serves as sample container, is glued on top of the SU-8 surface of the microcoil. The microcoil chip is bonded to a PCB, where the resonant circuit is implemented such that the microprobe can be connected directly to the preamplifier of a conventional NMR spectrometer.…”

Section: Experimental Spectroscopy Set-upmentioning

confidence: 99%

“…4 Microcontact printing can also be used when patterning living cells. Then, however, an extracellular matrix would be printed onto a surface.…”

Section: Spectroscopy Of Mammalian Cellsmentioning

confidence: 99%

“…2,3 In a previous contribution, we characterized the performance of our surface microprobe and demonstrated a dramatic improvement of the spin excitation uniformity by patterning artificial vesicles in the centre of the coil, where the rf-field is uniform. 4 We have demonstrated the feasibility of taking spectra of cells confined in a micro-channel with microfabricated Helmholtz coils as well. 5 However, with such a 3D confined structure, cell perfusion was not feasible due to the excessive pressure required to exchange the liquids.…”

Section: Introductionmentioning

confidence: 99%

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