2020
DOI: 10.1371/journal.pone.0236849
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Sample pooling methods for efficient pathogen screening: Practical implications

Abstract: Due to the large number of negative tests, individually screening large populations for rare pathogens can be wasteful and expensive. Sample pooling methods improve the efficiency of large-scale pathogen screening campaigns by reducing the number of tests and reagents required to accurately categorize positive and negative individuals. Such methods rely on group testing theory which mainly focuses on minimizing the total number of tests; however, many other practical concerns and tradeoffs must be considered w… Show more

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Cited by 17 publications
(12 citation statements)
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References 37 publications
(53 reference statements)
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“…Since its introduction in 1943 by Robert Dorfman ( 155 ), “pooling of samples” has become a commonly used method for the detection and diagnosis of infectious diseases ( 156 159 ). Specifically, pooling of samples means that different samples are mixed to form a pool before tests are performed on that pool as a single sample ( 160 162 ).…”
Section: Synoptic Overview Of Sars-cov-2 Rt-dpcr Applicationsmentioning
confidence: 99%
“…Since its introduction in 1943 by Robert Dorfman ( 155 ), “pooling of samples” has become a commonly used method for the detection and diagnosis of infectious diseases ( 156 159 ). Specifically, pooling of samples means that different samples are mixed to form a pool before tests are performed on that pool as a single sample ( 160 162 ).…”
Section: Synoptic Overview Of Sars-cov-2 Rt-dpcr Applicationsmentioning
confidence: 99%
“…Lately, combinatorial pooling strategies, like DoBSeq, have received increased attention as a theoretical alternative to the extensive and costly population screening for the SARS-Cov-2 virus 25 . While the methods appear similar, a crucial difference is that, a disease may be caused by a myriad of distinct genetic variants, whereas SARS-Cov-2 tests, by and large, are positive in the same way.…”
Section: Discussionmentioning
confidence: 99%
“…16,17 In addition to simple pooling testing (e.g., Dorfman testing or hierarchical group testing), more complicated methodologies such as nonhierarchical group testing or array testing have also been developed. [18][19][20] Recently, these pooling strategies have been adopted to efficiently detect SARS-CoV-2 by speeding up the tests and increasing the testing capacity, and have thus been recommended by the US FDA as well. 21 Several simulation studies tried to determine the optimal pooling strategy by taking into account relevant parameters such as pool size and positive rate (PR).…”
Section: Introductionmentioning
confidence: 99%
“…Accordingly, strategies for pooling diagnostic specimens, which have been applied in screening donated blood samples for blood‐borne pathogens such as hepatitis B virus, 14,15 were introduced to reduce the cost of screening large populations 16,17 . In addition to simple pooling testing (e.g., Dorfman testing or hierarchical group testing), more complicated methodologies such as nonhierarchical group testing or array testing have also been developed 18–20 . Recently, these pooling strategies have been adopted to efficiently detect SARS‐CoV‐2 by speeding up the tests and increasing the testing capacity, and have thus been recommended by the US FDA as well 21…”
Section: Introductionmentioning
confidence: 99%